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  • Discovery of The Tudor Plasmid From Drosophila Melanogaster

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    Tudor domains are found in many eukaryotic organisms and have been implicated in protein-protein interactions. Methylated protein substrates bind to these domains. Mutations on the SMN protein have been linked to spinal muscular atrophy.

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    PCR Analysis

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    Tudor domains are found in many eukaryotic organisms and have been implicated in protein-protein interactions. Methylated protein substrates bind to these domains. Mutations on the SMN protein have been linked to spinal muscular atrophy.

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    15 views6 pages

    Discovery of The Tudor Plasmid From Drosophila Melanogaster

    Uploaded by

    big5040
    Tudor domains are found in many eukaryotic organisms and have been implicated in protein-protein interactions. Methylated protein substrates bind to these domains. Mutations on the SMN protein have been linked to spinal muscular atrophy.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
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    of 6

    Discovery of the Tudor Plasmid from Drosophila Melanogaster

    By: Kaari Casey, Brooke Gushen, Ryan Snavely, and Idong Udoyen

    Methods to Find Plasmid Sequence


    1. 2. 3. 4. 5. 6. 7. 8. E. coli Colony Selection DNA Replication Plasmid DNA Removal PCR Amplification Gel Electrophoresis Plasmid DNA Selection DNA Sequencing Determining Unknown Gene

    Results Nucleotide BLAST

    Gel Sample one intense band Plasmid DNA Sequence from Nuclei Acid Facility NCBI BLAST Matches
    Drosophila: melanogaster, erecta, yakuba, simulans, sechellia

    Plasmid Results Tudor Gene

    3rd line- Drosophila melanogaster tudor (tud) mRNA, 8205 bp, 100%

    Protein Coding Tudor domains are found in many eukaryotic organisms and have been implicated in protein-protein interactions in which methylated protein substrates bind to these domains. -NCBI

    Human Homologue Results SMN Protein


    Survival Motor Neuron (SMN) Protein in Humans Very Important Tudor Domain for Binding Proteins Spinal Muscular Atrophy- Linked to SMN Protein; Mutation on SMN Gene

    Reference
    http://www.ncbi.nlm.nih.gov http://en.wikipedia.org http://www.bing.com (the lab manual)

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