miRNA

Present by Mr. V. Kuzhandai Velu

MOLECULAR CANCER 2010, 9:306

SERUM microRNA AS NON-INVASIVE BIOMARKERS FOR CANCER

Jan C Brase, Daniela Wutting, Ruprecht kuner, Holger sultman

The first miRNA was discovered in C. elegans.

lin-14(lf) mutations caused precocious development. lin-14(gf) and lin-4 mutations caused retarded development.
wild type: embryo preccious: embryo retarded: embryo L1 L2 L1 L2 L3 L1 L3 L4 L2 L4 adult adult L3..

Tree various types of RNA

What is mircoRNA?

Non Coding RNA Circulating RNA 18 24 nucleotides

DNA

Function ?

mRNA
microRNA

Gene Regulation Inhibition Regulation Switching off Genes

Protein

What are MicroRNAs?

MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate eukaryotic gene expression at the translation level

RISC = RNA-induced Silencing Complex

MicroRNA formation
miRNAs are processed from several precursor stages Mammalian genomes seem to have 100s of miRNAs

miRNA Biological Process

How is released in serum ?

Passive release injure to tissue

Eg. miRNA-208a Myocardial infection

Recent study showed released form microvesicles

Putative functional roles of circulating miR

Supporting cell cell communication along exosome Cermaide dependent secretory meachinery involves microvesicle based miR. Recent study showed released form microvesicles Cell can actively secrete endogenous miR

Reflect Physiological & Pathological change

S.No. 1. Physiological & Pathological condition Pregnant women microRNA miRNA-526a miRNA-527 miRNA-520d-5p

2.

Maternal (Placenta)

miRNA-141 miRNA-149 miRNA-299 5p

miRNA-208 miRNA-499

3.

Myocardial infraction

4.

Liver Injury

miRNA-122 miRNA-192
miRNA-146a miRNA-122

5.

Systemic Inflammation

Diagonstic markers
S.No. 1. Tumor Breast Cancer microRNA miRNA-195 let 7a miRNA-155 miRNA-29 miRNA-92a, 92 miRNA-17-3p miRNA-21 miRNA-126 miRNA-99 miRNA-141 miRNA-375 miRNA-210 miRNA-21

2.

Colon Cancer

3.

Ovarian Cancer

4. 5.

Prostate Cancer Pancreatic Cancer

Analyze

RT PCR PCR MICROARRAY