Name:

Date:

Period:

Experimental Design: Temperature and Enzymes Problem: How does temperature affect the rate of an enzyme-catalyzed reaction? Research and Observations: Some cells in your body can produce hydrogen peroxide (H202) to help fight infection. H202 is one of many chemicals that can help cells at low levels and harm them at high levels. So the level of H202 in a cell must be controlled H202 can break down into water (H20 )and oxygen gas (02). An enzyme called catalase helps speed up this reaction. In this lab, you will observe this enzyme-catalyzed reaction. Then you will design an experiment to determine the effect of temperature on the reaction. Safety Precautions: Hydrogen peroxide solution can be irritating. If the solution gets in your eye, tell your teacher and she will send you to the nurse. Vocabulary: Chemical reactions Reactants Products Activation Energy Catalyst Enzymes Substrates

Watch the Demonstration: Write your observations here on how liver reacts with Hydrogen Peroxide

Hypothesis (At what temperature will the enzyme catalase function the best):
If , then

Name:

Date:

Period:

Independent Variable

Dependent Variable

Constants

Control

Materials:
1. 2. 3. 4. 5. 6. 7. 8.

Procedure:

Pureed liver Forceps 1% hydrogen peroxide solution 50-mL beakers Filter paper disks Timer Water baths Thermometers

Write a step-by-step procedure for your experiment. Have Ms. Patton or Ms. Maria approve it before you start.

Analysis and Conclusion: (Attach the graph to the back of this page)