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  • Coloração de Gram

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    Catarina Leão
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    3. Coloração de Gram

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    13 views5 pages

    Coloração de Gram

    Uploaded by

    Catarina Leão

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 5

    COLORAO DE GRAM

    Hans Christian Gram 1884 Colorao de Gram utilizada para diferenciar bactrias em dois grandes grupos (Grampositivo e Gram-negativo) baseado nas propriedades fsicas e caractersticas qumicas de suas paredes celulares. Colorao composta, a frio, diferencial.

    Parede celular bacteriana

    GRAM-POSITIVA

    GRAM-NEGATIVA

    Colorao de Gram
    1. CRISTAL VIOLETA

    2. LUGOL
    3. LCOOL 4. FUCSINA

    Preparar esfregao Secar Fixar na chama 1. CRISTAL VIOLETA 1 min. 2. LUGOL 1 min.

    3. LCOOL
    4. GUA
    5. FUCSINA 45 seg.

    6. GUA
    Secar lmina Colocar 1 gota de leo de imerso. Observar com objetiva de imerso (100X)

    E. coli Gram-

    Pseudomonas Gram-

    Staphylococcus Gram+

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