International Senior Capstone Design Project | ATLANTIS - University of Arizona Project title: Automated Zebrafish Tracking using MATLAB

Project by: Laura-Ann Chin, Richard Lucio III, Kevin Newman & Steve Peugh Fall 2009 / Spring 2010 | Website: zebrafish.co.nr

Group meeting: April 26, 2010 Monday 1.15pm-3pm Subject: Mock Preso & Poster Revision Session Project Purpose Control experiment vs. experiment under the influence of neurotoxins Zebrafish commonly used in medical experiments Translucent, allows for the study of organ development and bone cartilage staining Project/Technical Requirements Spoke with customer (Dr. Horacio Rilo UA College of Medicine), who heads Zebrafish experiments to understand functions, uses & users of program Develop cost-effective, user intuitive & open source program Graphical User Interface GUI - MATLAB : wide spread Generate a 3D graph of Zebrafish position Calculate velocity and acceleration data process data & plot Program should be adaptive to new and changing environments Design/ Model GUI: Input by user, acquire frames, program performs background subtraction, plots data Talk about tank and camera setup (camera mount (stable), axes adjustable, tank in cubic environment (easier to control), etc) Talk about general programming outline Video output with centroid markers, display Team Member Topic of Discussion Laura-Ann Chin Project definition/purpose Richard Lucio III Details, program function (how GUI graphs & how it is manipulated) Part of conclusion Kevin Newman Steve Peugh Stability, GUI, General Project requirements Programming Outline

Part of conclusion

Part of conclusion

Part of conclusion

Contact Information: UA Advisor Prof. Eniko Enikov enikov@email.arizona.edu

STUBA Advisor Prof. Lubos Magdolen Lubos.Magdolen@stuba.sk

Project Researchers Laura-Ann C. lchin@email.arizona.edu Richard L. rlucio@email.arizona.edu Kevin N. knewman@email.arizona.edu Steve P. sbpeugh@email.arizona.edu