Gena SMP 2

Materia : PAN Profesor coordonator : Farasanu Ileana Studenta : Mitran Corina Mihaela Facultatea : Facultatea de Chimie, sectia BTH , an III , sem. II , 2013 , Bucuresti

Denumirea genei :
Numele standard : PAH1 Denumirea sistematica : YMR165C Denumirea uzuala : SMP2 Denumirea stiintifica : Acid fosfatidic fosfo hidrolaza

Descrierea genei :
contin ioni de Mg 2+ ce sunt dependente de fosfatid fosfatazele ( PA ) . Este implicata in defosforilarea fosfatid fosfatazelor ( PA ) pana la diacilglicerol . este implicata in sinteza novo a lipidelor si formarea de picaturi de lipide .

prin fosforilare de Pho80p-Pho85p scade activitatea catalitic i modifica Pah1p unde sunt localizare i se gasesc din abundena .

scade eficienta catalitica prin fosforilarea de protein kinaza A .

Activitatea genei :
Functia moleculara : este implicata in activitatea fosfatid fosfatazelor . - Catalizeaza reactia : 1,2-diacylglycerol 3-phosphate + H2O => 1,2-diacyl-sn-glycerol + phosphate.

Locatia cromozomala :
Harta caracteristicilor cromozomiale
Features around YMR165C on chromosome XIII Spanning a region 10kb left and 10kb right(coordinates 580040 to 602628)

Fenotipuri mutante :
1) Genetica clasica : a ) Conditionate : - acumularea anormala a fosfolipidelor ; - acumularea anormala a lipidelor ; - morfologie nucleara anormala ; - scaderi ale utilizarii surselor de carbon ; b ) Nule : - morfologie vacuolara anormala ; - morfologia nucleara anormala ; - morfologia reticulului endoplasmatic anormala; - acumularea fosfatidilinozitol 3- fosfat absenta ; c ) Nespecificate : - rezistenta in fata toxinelor scade;

AGTCAGTACGTAGGCAGAGCTCTTGGGTCTGTGTCTAAAACATGGTCTTCTATCAATCCGGCTACGCTATCAGGTGCTATAGATGTC ATTGTAGTGGAGCATCCAGACGGAAGGCTATCATGTTCTCCCTTTCATGTGAGGTTCGGCAAATTTCAAATTCTAAAGCCATCTCAA AAGAAAGTCCAAGTGTTTATAAATGAGAAACTGAGTAATATGCCAATGAAACTGAGTGATTCTGGAGAAGCCTATTTCGTTTTCGA GATGGGTGACCAGGTCACTGATGTCCCTGACGAATTGCTTGTGTCGCCCGTGATGAGCGCCACATCAAGCCCCCCTCAATCACCTG AAACATCCATCTTAGAAGGAGGAACCGAGGGTGAAGGTGAAGGTGAAAATGAAAATAAGAAGAAGGAAAAGAAAGTGCTAGA GGAACCAGATTTTTTAGATATCAATGACACTGGAGATTCAGGCAGTAAAAATAGTGAAACTACAGGGTCGCTTTCTCCTACTGAAT CCTCTACAACGACACCACCAGATTCAGTTGAAGAGAGGAAGCTTGTTGAGCAGCGTACAAAGAACTTTCAGCAAAAACTAAACA AAAAACTCACTGAAATCCATATACCCAGTAAACTTGATAACAATGGCGACTTACTACTAGACACTGAAGGTTACAAGCCAAACAAG AATATGATGCATGACACAGACATACAACTGAAGCAGTTGTTAAAGGACGAATTCGGTAATGATTCAGATATTTCCAGTTTTATCAAG GAGGACAAAAATGGCAACATCAAGATCGTAAATCCTTACGAGCACCTTACTGATTTATCTCCTCCAGGTACGCCTCCAACAATGGCC ACAAGCGGATCAGTTTTAGGCTTAGATGCAATGGAATCAGGAAGTACTTTGAATTCGTTATCTTCTTCACCTTCTGGTTCCGATACT GAGGACGAAACATCATTTAGCAAAGAACAAAGCAGTAAAAGTGAAAAAACTAGCAAGAAAGGAACAGCAGGGAGCGGTGAGA CCGAGAAAAGATACATACGAACGATAAGATTGACTAATGACCAGTTAAAGTGCCTAAATTTAACTTATGGTGAAAATGATCTGAAAT TTTCCGTAGATCACGGAAAAGCTATT GTTACGTCAAAATTATTCGTTTGGAGGTGGGATGTTCCAATTGTTATCAGTGATATTGAT GGCACCATCACAAAATCGGACGCTTTAGGCCATGTTCTGGCAATGATAGGAAAAGACTGGACGCACTTGGGTGTAGCCAAGTTAT TTAGCGAGATCTCCAGGAATGGCTATAATATACTCTATCTAACTGCAAGAAGTGCTGGACAAGCTGATTCCACGAGGAGTTATTTGC GATCAATTGAACAGAATGGCAGCAAACTACCAAATGGGCCTGTGATTTTATCACCCGATAGAACGATGGCTGCGTTAAGGCGGGA AGTAATACTAAAAAAACCTGAAGTCTTTAAAATCGCGTGTCTAAACGACATAAGATCCTTGTATTTTGAAGACAGTGATAACGAAGT GGATACAGAGGAAAAATCAACACCATTTTTTGCCGGCTTTGGTAATAGGATTACTGATGCTTTATCTTACAGAACTGTGGGGATACC TAGTTCAAGAATTTTCACAATAAATACAGAGGGTGAGGTTCATATGGAATTATTGGAGTTAGCAGGTTACAGAAGCTCCTATATTCA TATCAATGAGCTTGTCGATCAT TTCTTTCCACCAGTCAGCCTTGATAGTGTCGATCTAAGAACTAATACTTCCATGGTTCCT GGCTCCCCCCCTAATAGAACGTTGGATAACTTTGACTCAGAAATTACTTCAGGTCGCAAAACGCTATTTAGAGGCAATCAGGAAGA GAAATTCACAGACGTAAATTTTTGGAGAGACCCGTTAGTCGACATCGACAACTTATCGGATATTAGCAATGATGATTCTGATAACAT CGATGAAGATACTGACGTATCACAACAAAGCAACATTAGTAGAAATAGGGCAAATTCAGTCAAAACCGCCAAGGTCACTAAAGCC CCGCAAAGAAATGTGAGCGGCAGCACAAATAACAACGAAGTTTTAGCCGCTTCGTCTGATGTAGAAAATGCGTCTGACCTGGTGA GTTCCCATAGTAGCTCAGGATCCACGCCCAATAAATCTACAATGTCCAAAGGGGACATTGGAAAACAAATATATTTGGAGCTAGGTT CTCCACTTGCATCGCCAAAACTAAGATATTTAGACGATATGGATGATGAAGACTCCAATTACAATAGAACTAAATCAAGGAGAGCAT CTTCTGCAGCCGCGACTAGTATC GATAAAGAGTTCAAAAAGCTCTCTGTGTCAAAGGCCGGCGCTCCAACAAGAATTGTTTCA AAGATCAACGTTTCAAATGACGTACATTCACTTGGGAATTCAGATACCGAATCACGAAGGGAGCAAAGTGTTAATGAAACAGGGC GCAATCAGCTACCCCACAACTCAATGGACGATAAAGATTTGGATTCAAGAGTAAGCGATGAATTCGATGACGATGAATTCGACGAA GATGAATTC GAAGATTGA .

Am taiat cu AccI
Cut Site Fragment size ( bp ) 1926 1926 663

2589

Bibliografie
1) 1https://www.google.ro/search?hl=ro&site=imghp&tbm=isch&source=hp&biw=1178&bih=620&q=logo+s gd&oq=logo+sgd&gs_l=img.3..0i19.901.15425.0.16673.8.8.0.0.0.0.140.874.2j6.8.0...0.0...1ac.1.9.img.o7c2 1irzrzg#hl=ro&site=imghp&tbm=isch&q=saccharomyces+genome+database&spell=1&sa=X&ei=GkZ1UbnQ EYiMOPO7gcAJ&ved=0CEoQvwUoAA&bav=on.2,or.r_cp.r_qf.&bvm=bv.45512109,d.ZWU&fp=8c6da28ac96 f2ec0&biw=1178&bih=620&imgrc=LRKPdu2M0YOfaM%3A%3BybNUAPS1_7iXvM%3Bhttps%253A%252F% 252Fsi0.twimg.com%252Fprofile_images%252F1752488737%252Ftwitter_logo_3.gif%3Bhttps%253A%252 F%252Ftwitter.com%252Fyeastgenome%3B160%3B160 2 ) http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=SMP2

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