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    The document describes two chemical tests, the Salkowski reaction and Tschugajeu test, to identify phytosterols and triterpenoids respectively in a plant sample. The Salkowski reaction involves adding concentrated sulfuric acid to the sample and observing a reddish brown color in the chloroform layer, indicating phytosterols. The Tschugajeu test adds excess acetyl chloride and zinc chloride, then heating, and a positive result is an eosin red color, showing the presence of triterpenoids.

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    For Chemical Test of terpenoids

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    The document describes two chemical tests, the Salkowski reaction and Tschugajeu test, to identify phytosterols and triterpenoids respectively in a plant sample. The Salkowski reaction involves adding concentrated sulfuric acid to the sample and observing a reddish brown color in the chloroform layer, indicating phytosterols. The Tschugajeu test adds excess acetyl chloride and zinc chloride, then heating, and a positive result is an eosin red color, showing the presence of triterpenoids.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
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    49 views1 page

    For Chemical Test

    Uploaded by

    amritaryaaligargh
    The document describes two chemical tests, the Salkowski reaction and Tschugajeu test, to identify phytosterols and triterpenoids respectively in a plant sample. The Salkowski reaction involves adding concentrated sulfuric acid to the sample and observing a reddish brown color in the chloroform layer, indicating phytosterols. The Tschugajeu test adds excess acetyl chloride and zinc chloride, then heating, and a positive result is an eosin red color, showing the presence of triterpenoids.

    Copyright:

    Attribution Non-Commercial (BY-NC)
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    3.1.1 Salkowski reaction To 0.5 ml sample in a test tube add 1ml of Conc.

    H2SO4 from the sides of the test tubes. Appearance of reddish brown colour in chloroform layer indicates presence of phytosterols. 3.2 TEST FOR TRITERPENOIDS 3.2.1 Tschugajeu test To the sample, add excess of acetyl chloride and a pinch of zinc chloride. Keep aside for reaction to subside and then warmed on water bath. Appearance of eosin red colour indicates presence of triterpinoids.

    1. Harbone Jb. Phytochemical Methods. Edn 3. Chapman And Hall, London.1998; p 117-119. 2. Fransworth, N.R. Biological and Phytochemical Screening of Plants, Journal of Pharmaceutical Science. 1996; 55:225-227. 3. Vinod.D, Rangari.Pharmacognosy And Phytochemistry, Part-1, Edn 1, Carrier Publication, Nasik. 2002; Pp.132.

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