Partial Pressures of Gas Mixtures You will need the following conversion for parts B and C: 1 mm of Hg = 13.

6 mm of H2O Pre-Lab Questions 1. What is meant by the term partial pressure? The individual pressures exerted by each of the gases that make up the total atmospheric pressure. A. Partial Pressures of Oxygen and Nitrogen in Air (use Partial Pressures exercise module for a simulation of this experiment) A.1 Volume of test tube = 78.0 mL A.2 Volume of nitrogen = 62.0 mL A.3 Atmospheric pressure = 754 mm Hg Calculations: A.4 Volume of oxygen 78.0mL - 62.0mL = 16.0mL O2 A.5 Percent oxygen (show calculation) 16.0 78.0 * 100 = 20.5% O2 Percent nitrogen (show calculation) 62.0 78.0 * 100= 79.5% N2 A.6 Partial pressure of oxygen 754 mm Hg * 20.5 = 154.57 O2 100 Partial pressure of nitrogen 754 mm Hg * 79.5 = 599.43 N2 100 Questions and Problems Q.1 If you lived in the mountains where the atmospheric pressure is 685 mm Hg, what would the partial pressure of oxygen and of nitrogen be? Use your % values from A.5. (show calculation) 685 mm Hg * 20.5 = 140.43 O2 100 685 mm Hg * 79.5 = 545.37 N2 100 B. Carbon Dioxide in the Atmosphere B.1 Atmospheric pressure = 754 mm Hg

B.2 PCO2 (height of water column in tube B) = 6 mm H2O Calculations B.3 PCO2 6mm Hg * 1mm Hg = 0.44mm Hg 13.6 mm H2O B.4 Percent CO2 in the atmosphere __0.058_____ % CO2 (atmosphere) Show calculations: 0.44mm Hg * 100 = 0.058% CO2 754mm Hg C. Carbon Dioxide in Expired Air C.1 Atmospheric pressure = 754 mm Hg C.2 Height of water column = 27.0 mm H2O Calculations C.3 PCO2 Show calculations: 27.0 mm H2O * 1 mm Hg = 1.99 mm Hg 13.6 mm H20 C.4 Percent CO2 (expired air) __0.26_____% CO2 (expired air) (Show calculations) 1.99 mm Hg * 100 = 0.26% CO2 754 mm Hg Questions and Problems Q.2 Which would you expect to be higher, the percentage of CO2 in the atmosphere or in expired air? Why? Expired air because expiration occurs when the diaphragm relaxes and moves back up into the thoracic cavity to its resting position. This reduces the volume of the thoracic cavity which squeezes the lungs and decrease their volume. The pressure in the lungs is higher than the pressure of the atmosphere.

Q.3 What is the total pressure in mm Hg of a sample of gas that contains the following gases: O2 (45 mm Hg), N2 (1.20 atm), and He (825 mm Hg)? Show work or explain your thought process. 1 atm = 760 mm Hg N2= 1.2 atm * 760 mm Hg = 912 mm Hg Total Pressure = 45 mm Hg + 912 mm Hg + 825 mm Hg= 1,782 mm Hg

Q.4 A mixture of gases has a total pressure of 1650 mm Hg. The gases in the mixture are helium (215 mm Hg), nitrogen (0.28 atm), and oxygen. What is the partial pressure of the oxygen in atm? Show work or explain your thought process. 1 atm = 760 mm Hg Nitrogen= 0.28 atm * 760 mm Hg = 212.8 mm Hg Total Pressure = 215 mm Hg + 212.8 mm Hg + X = 1650 mm Hg 1650 mm Hg - 427.8 = 1222.2 mm Hg O2 Percent of O2 Partial Pressure of O2 1222.2 * 100 = 74.1% O2 1650 * 74.1 = 1222.65 1650 10