SANDEEP MALLAREDDY

505, Sunnyside Homes, Opp. Apollo Hospitals, Waltair Main Road, Visakhapatnam-530002.

Tel: (91) 9908166652. Email: m_sandeep85@yahoo.com

-------------------------------------------------------------------------------------------------------------------------------

OBJECTIVE

To apply my knowledge and experience in solving biological problems and developing

sustainable and novel technologies.

EDUCATION

Master of Science In Biology (GPA 4.0/4.0) [Aug 2006 to Dec 2008]

University of Kentucky, Lexington, Kentucky USA.

Bachelor of Technology in Biotechnology (80.81%) [Aug 2002 to May 2006]

Godavari Institute of Engineering and Technology, Rajahmundry, A.P.

Intermediate Education[ M.P.C.] (91.2%) [Aug 2000 to May 2002]

Narayana Junior College, Aseelmetta , Visakhaptanam, A.P.

High School (ICSE) (83.5%) [May 2000]

St.Josephs Secondary School, Malkapuram, Visakhaptanam A.P.

EMPLOYMENT

BIO 153 Teaching Assistant [Aug 2006 to Dec 2006]

Introductory laboratory for undergraduates dealing with the various aspects of biological
systems at the organismal, population and community levels

Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A.

Duties included general maintenance of the lab, lectures and conducting experiments and
analysis of data, conducting examinations and grading.

BIO151 Teaching Assistant [Jan 2007 to May 2007]

Introductory laboratory for undergraduates in which biological systems are investigated at the
cellular and molecular levels.

Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A.

Duties included general maintenance of the lab, lectures and conducting experiments and
analysis of data, conducting examinations and grading.
BIO 510 Recombinant DNA Technology Lab Teaching Assistant [Aug 2007 to Dec 2007]
[Aug 2008 to Dec 2008]
Advanced laboratory course for undergraduates and graduate students in construction,
isolation, and analysis of recombinant DNA clones, with emphasis on practical experience in
basic techniques.

Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A.

Duties included general maintenance of the lab, ensuring proper conduct of experiments with
minimal supervision. Ordering supplies and making creative choices for the smooth operation of
the course.

BIO 209 Introductory Microbiology Lab [Jan 2008 to May 2008]

Introductory laboratory for undergraduates introducing fundamental microbiological principles
and techniques.

Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A.

Duties included general maintenance of the lab, lectures and conducting experiments and
analysis of data, conducting examinations and grading.

RESEARCH ASSISTANT [May 2007 to Aug 2007]

[May 2008 to Aug 2008]
Employer: Dr. Peter .M. Mirabito, Department Of Biological Sciences, University Of Kentucky, KY

Duties included maintenance of the lab, Designing and implementing experiments,

administration and inventory management, and training undergraduate students[KBRIN
program] in the lab.

RESEARCH EXPERIENCE

The Role of TLH genes in telomere maintenance [Jan 2007 to Dec 2008]

The ends of linear chromosomes have evolved into specialized, multi-functional chromatin domains.
These domains include the telomere proper, which typically consists of simple repetitive DNA sequences
and the proteins that bind to them, and the subtelomeric region, a less well-defined domain which often
includes more complex repetitive DNA elements. Telomeric repeats and their binding proteins are well
conserved and their function is, in principle, understood. In contrast, subtelomeric elements vary
among species and even among varieties of a single species, and their functions are unknown. We are
using Aspergillus nidulans to study the function of telomere-linked helicase (TLH) genes, which are
conserved, fungal-specific subtelomeric sequences. As part of our investigation to study tlhA
function,multiple tlhA::Af pyrG mutants were created, each of which exhibited the same two
phenotypes: slow colony growth and defective ascosporogenesis. Based on our observations we
speculate that telomeric heterochromatin represses TLH and other subtelomeric genes during
vegetative growth and further speculate that changes in telomere structure during ascosporogenesis
underlie the enhanced TPE phenotype we observe during sexual reproduction. This work was done
under the guidance of Dr. Peter M. Mirabito, Department of Biological Sciences, University of
Kentucky.
Interaction of CG14011 product with HOAP protein [Aug 2006 to Dec 2006]
Drosophila Heterochromatin protein (HP1) complex contains subunits of the Origin Recognition
Complex, known to bind and recruit DNA replication factors to specific sequences in the initiation of
DNA replication. The association of this heterochromatin protein with DNA replication proteins suggests
a mechanism for linking the process of heterochromatin assembly to that of DNA replication. This
complex contains a second previously uncharacterized DNA-binding activity that we have designated as
HP1/ORC-Associated Protein (HOAP). We have found this protein to function in the assembly of a
specific heterochromatic structure at chromosome ends that prevents them from undergoing end-to-
end fusions. A Two Hybrid Screen of cDNA library with HOAP gave a list of possible candidates of which
CG14011 is a testes specific gene. To confirm the interaction of the HOAP protein with CG14011 gene
product, an co precipitation assay was done using radioactively in vitro translated CG14011 gene and
HOAP protein. The result of interaction between the two proteins have been conclusively
demonstrated. This work was done under the guidance of Dr. Rebecca Kellum, Department of
Biological Sciences, University of Kentucky.

Reconstitution of Dihaploids from Leaf Tissue of Anther Derived Haploids and Molecular
Characterization [January 2006-June 2006]

Haploid plants have many uses in genetics and plant breeding research. There are numerous ways of
producing individuals which include twin selection, wide crossing, and in vitro techniques such as anther
culture, pollen culture and ovule culture, which rely on our ability to divert tissues with gametic
(haploid, n) chromosome number to develop as sporophytes while retaining the haploid chromosome
complement. However, haploid plants are shorter than diploids, weaker, and sterile because of
univalent chromosomes. However, it happens with some haploid plants that the meiotic division takes
place in macrosporogenesis and microsporogenesis and these results in spontaneous development of a
fertile diploid plant. It was indicated that ‘aged’ plant portions might be useful in developing diploids by
way of tissue culture techniques. Thus the problem was to locate cells, in selected haploid plants, that
were capable of dividing and organizing into normal diploid plants. Several plant components were
tested to determine their usefulness in production of diploids from haploids that had grown to the
flowering stage as part of their selection procedure. It was found that leaf mid veins from aged leaves
(those that remained on the plants 3 to 4 weeks after attaining full expansion) when cultured in
chemically defined media gave rise to diploids which were normal in appearance; self fertile and
produced diploid progeny. In a period of 3 months, anther derived haploids were raised and were
subjected to leaf mid vein culture by optimizing media favoring development of dihaploids. A large
number of haploids have been cultured to produce dihaploids. This work was done under the guidance
of Dr.Sarala, Biotechnology Division, Central Tobacco Research Institute, Rajahmundry.

RELATED COURSEWORK

Advanced Cell Biology, Bioinformatics, Molecular genetics , Advanced genetic analysis,

Biostatistics, Experimental Genetics, Quantitative Analysis, Advanced Biochemistry, Probability
and Statistics, Molecular modeling and Drug Design, Microbiology, Thermodynamics, Mass
Transfer Operations, Chemical reaction engineering, Bioorganic Chemistry, Biopharmaceutical
Technology, Food Technology, Industrial Microbial technology, Protein Engineering, Enzyme
Technology.
RELEVANT SKILLS

Chemistry

Basic operation and maintenance of spectrophotometers (i.e. Spec 21 and UV-vis) to monitor
growth of microbial cultures and to assay enzymatic activities.
Skillful in preparation of various stock solutions and reagents including anaerobic stocks.

Microbiology

Preparation of various media including anaerobic to support growth of microorganisms and

operation of autoclaves.
Sterile technique in isolation and transfer of microorganisms.
Maintenance and storage of aerobic and anaerobic microbial cultures.
Basic operation and maintenance of bright field, fluorescent and phase contrast microscopes.
Basic staining techniques and performing various microbiological tests like IMVAC.
Preparation of various fungal media and handling of pathogenic strains of fungi.

Molecular Biology

Gel Electrophoresis of DNA and RNA

SDS PAGE electrophoresis techniques
PCR including Inverse PCR, Real Time PCR, Reverse Transcriptase PCR, Nested PCR.
RAPD and RFLP.
Site Directed and Random Mutagenesis using Chemical and PCR methods.
Pulse Field (CHEF) gel Electrophoresis
Northern , Western and Southern Blots using radioactive and non radioactive Probes.
DNA and RNA extraction from eukaryotic and prokaryotic cells including whole chromosome
extraction.
Achilles Heel RecA mediated cleavage of whole chromosomes.
Restriction Digestion, Ligation, Cloning and Transformation techniques.
Two Hybrid Colony screening.
Proficient in Use of recombineering technologies like Cre-Lox, GATEWAY, TOPO cloning.
Co precipitation of proteins.
Expression and isolation of Tagged Proteins from Bacterial and Eukaryotic cells.
Sequencing using automated sequencers and interpretation and assembly of contigs.
Handling of phage and eukaryotic vectors.
Proficient in FISH techniques and various other assays.
Plant tissue culture.
Extensive knowledge in various Bioinformatic tools and services like NCBI, Swiss PDB, EMBL,
RasMol, BLAST, CLUSTALW, VectorNti.
 Computer

Proficiency in operation of personal computers and software packages like Microsoft Office.
Use of MS Excel to create spreadsheets, graphs and statistically analyze data (i.e. averages,
standard deviation, student t-test, linear regression analysis).
Use of MS Access to create databases and maintain them.
Literature searches through programs such as PubMed.
Use of bibliography management tools like EndNote.

Supervisory Skills

Managed group lab sessions and evaluated a total of 180 undergraduate students over 5
semesters.
Monitored activity of students and ensured safety provisions were used.
Ordered lab supplies and maintained general business of the lab.

ACADEMIC AWARDS

“Flora G. Ribble Scholarship” for the year 2007 and 2008, Department of Biology, University of
Kentucky, Lexington, Kentucky.

Branch Topper in Biotechnology in Third Year Second Semester at GIET, Rajahmundry.

State Rank (19) in Biotechnology from JNTU, Hyderabad.

First Prize in Working Model presentation for Project Titled ‘ETHANOL PRODUCTION USING
AGRICULTURAL WASTE’ at SRUJANA 2003, Hyderabad

GRE Exam (1410/1600), TOEFL (290/300)

CONFERENCES AND WORKSHOPS

Work shop on “Bio Informatics and tools and applications” at the Indian Institute of Technology
(IIT), New Delhi.
[February23-27, 2005]

47th Annual American Society of Cell Biology Meeting, Washington D.C.

[December 1-5,2007]

Cellular & Molecular Fungal Biology, Gordon Research Conferences, Holderness, NH.
[June 29-July 3,2008]

Kentucky Academy of Sciences Annual Meeting 2008, Lexington, KY.

[Nov 1-Nov 3, 2008]
POSTERS

Telomeres, Telomere Crisis, and Telomere-linked RecQ Helicases in Aspergillus nidulans

S. Mallareddy, D.W. Perry, G. Li, M. Farman, P. M. Mirabito; 47th Annual American Society of Cell Biology
Meeting, Washington D.C., December 1-5,2007.

A Telomere Position Effect During Vegetative Growth and Ascosporogenesis in Aspergillus nidulans
Sandeep Mallareddy, Mark Farman and Pete Mirabito; Cellular & Molecular Fungal Biology, Gordon Research
Conferences, Holderness, NH. June 29-July 3,2008.

Genetic Analysis of RecQ Helicase Function in Aspergillus nidulans

Christopher Allen, Jordan Robinson, Kayla Kinker, Sandeep Mallareddy, and Pete Mirabito.
KBRIN/INBRE Summer Undergraduate Research Program, Department of Biology, University of
Kentucky, Lexington, Kentucky 40506-0225

PERSONAL SKILLS

I am a highly motivated individual with good technical and management skills complimented by
excellent verbal and written skills. I am a fast learner with positive attitude and self confidence and can
easily adapt to new situations.

REFERENCES

Dr. Peter M Mirabito, Associate Professor, Dept of Biology , UKY(pmmira00@email.uky.edu)

Dr. Brian Rymond, , Professor ,Director of Graduate Studies , Dept of Biology , UKY (rymond@uky.edu)

Dr. Rebecca Kellum, Assistant Professor, Dept of Biology , UKY (rkellum@uky.edu)

Mr. John Seabolt , Course Administrator , Dept of Biology , UKY (jpseab01@email.uky.edu)

Dr.P.Suryanarayana Raju, H.O.D, Biotechnology, Godavari Institute of Engineering and technology,

Rajahmundry