Biotechnology Frontier

March 2012, Volume 2, Issue 1, PP.1-6

Research of the Effects of Dielectric Barrier

Discharge on Human Lymphocytes
Xingmin Shi 1#, Guanjun Zhang 2, Xili Wu 3, Zhengshi Chang 2, Zhuoyuan Dong 2, Wenlong Liao 2
1. School of Public Health of Xian Jiaotong University, Xian 710004, China
2. State Key Lab of Electrical Insulation and Power Equipment, Xian Jiaotong University,
3. Second Affiliated Hospital of Medical School of Xian Jiaotong UniversityXian 710004,China;
#Email:shixingmin142@163.com

Abstract
Peripheral blood lymphocytes (PBLC) isolated from 20 healthy adult volunteers blood samples were treated with low-temperature
plasma induced by dielectric barrier discharge, in order to investigate the effect of plasma on PBLC. Three experimental groups of
PBLC treated with plasma were classified according to their locations laid on the bottom electrode, i.e., the electrode center, the
electrode edge (2.5cm away from electrode center) and the electrode outside (on a platform with the same height to bottom
electrode and 5.0cm away from electrode center) group, and one control group was without plasma treatment. Three different
treatment time intervals were set as 5, 15 or 30s, respectively. Based on the statistical analysis of experimental results, it is revealed
that, while the lymphocytes were more than 5cm away from electrode center or the treatment time was less than 5s, their function
did not change significantly. Otherwise, the larger the dosage of employed plasma along with longer treatment time was; the more
significantly its function reduced. Furthermore, the lymphocytes apoptosis and necrosis caused by Low-temperature plasma
maybe result in the degradation of lymphocytes function.
Keywords: Dielectric Barrier Discharge (DBD); Low-temperature Plasma; Apoptosis

*
1 #, 2, 3, 2 2 2
1., 710061
2., 710049
3., 710004

(n=20)
2.5cm 5cm3 1 5s15s 30s 3
>5cm <5s
<5cm >5s

[1]
[2-4]
*

511250292010K16-04
xjj2012132 and xjj20100160
-1www.ivypub.org/bf


[5,6]
[7]

(DBD)
PBLC

1
1.1
1 10kV
10cm 1mm
3mm R3.9 C0.47F
DPO3034 2 VP 10kV

1 2 3

2 VP=10kV

1.2 PBLC
PBLC 20 10 10 30 6
5mL 1.077 [8] D-Hanks PBLC 1108
/mL.

1.3 PBLC
20 60%
2cm2cm 12 30ul
5s15s30s 4
PBLC
2.5cm 1

1.4
100ul 20% 1640 96
MTT [9]
-2www.ivypub.org/bf

OD 560nmSI
SI=ConA OD / OD

1.5
30s 106
/mL, 9 Eppendorf 1 0.4%3min
400
[10]

1.6
3mL PBS 30s 1000r/min 10min
3 250uL
106 /mL 100uL 5mL 5uLAnnexin-V 10uL PI
15min 400uLPBS

1.7
5 x SD
SPSS 13.0

2
2.1

1 5s SI

P>0.05 SI
P>0.05 15s 30s SI
P>0.05, SI P<0.05
SI P<0.05
>5cm
<5cm
1 PBLC SI
SI P>0.055s15s30s
SI SI P<0.05
5s15s30s<5s >5s
,
1 PBLC SI n=20

(s)
5

1.120.26

1.120.24

1.100.28

1.100.38
*
P<0.05 vs

2.2

15
1.120.25
0.710.10*
0.820.23*
1.100.32

30
1.120.26
0.580.15*
0.630.11*
1.100.35

3a 3b
-3www.ivypub.org/bf

 30s
3c 30s
3d 30s
3a

(b)

(a)

(c)

(d)

3 400

2.3
4 Annexin-V PI
4
30s P>0.05,
P<0.05
P<0.05

40

35
(%)

30
25
20
15
10

5
0

4 30s

*P<0.05 vs

3
5cm5s15s30s
2.5cm 15s 30s
5s 15s 30s
30 s
15s
Sosnin [11]
0.2W>10s
0.3W Sosnin
-4www.ivypub.org/bf


[7,12,13]
1 V

[14,15]

4 30s , ,
OH NO
[16,17]

4
DBD

1
2 3

REFERENCES
[1] M. Laroussi. Nonthermal Decontamination of Biological Media by Atmospheric-Pressure Plasmas: Review, Analysis, and
Prospects [J]. IEEE Trans.Plasma Sci., 2002, 30(4): 1409-1415.
[2] X.M. Shi, G.J. Zhang X.L. Wu, et al. Effect of Low-Temperature Plasma on Microorganism Inactivation and Quality of FreshlySqueezed Orange Juice[J].IEEE Transactions on Plasma Science,2011, 39(7):1591-1597.
[3] S.A. Ermolaeva, A.F. Varfolomeev, M.Y. Chernukha, et al.. Bactericidal Effects of Non-Thermal Argon Plasma in vitro, in
Biofilms and in the Animal Model of Infected Wounds [J].J Med Microbiol., 2011, 60(1):75-83.
[4] X.M. Shi, Y.Z. Sun, L.J. Yang, et al. Experimental Research of Injurious Effect of the Plasma Air Sterilization Machine on
Microbe in the Air [J]. High Voltage Apparatus, 2005, 41(5):333-335.
[5] J. Heinlin, G. Isbary, W. Stolz, et al. Plasma Applications in Medicine with a Special Focus on Dermatology [J]. Journal of the
European Academy of Dermatology and Venereology, 2011, 25(1):1-11.
[6] G. Fridman, M. Peddinghaus, H. Ayan, et al. Blood Coagulation and Living Tissue Sterilization by Floating-Electrode Dielectric
Barrier Discharge in Air [J]. Plasma Chemistry and Plasma Processing, 2006, 26(4):425-442.
[7] X. T. Deng, J. J. Shi, M. G. Kong. Physical Mechanisms of Inactivation of Bacillus subtilis Spores Using Cold Atmospheric
Plasmas [J]. IEEE Trans.Plasma Sci., 2006, 34(4): 1310-1316.
[8] S. Nagarajan, K. Venkiteswaran, M. Anderson, et al. Cell Specific, Activation Dependent Regulation of Neutrophil CD32A Ligand
Binding Function [J]. Blood, 2000, 95(10):69-77.
[9] L.P. Zhu,X.Q.Chen. Common Experimental Methods of Immunology [M].Beijing: People's Medical Publishing House, 2000:193-194.
[10] X.Yu, G.Y.Dong, Y.P.Qiao, et al. Comparative of Apoptosis of Morphological Examination [J]. Chinese Journal of Microecology,
-5www.ivypub.org/bf

2003, 15 (3): 140.

[11] E. A. Sosnin, E. Stoffels, M. V. Erofeev, et al. The Effects of UV Irradiation and Gas Plasma Treatment on Living Mammalian
Cells and Bacteria: a Comparative Approach [J]. IEEE Trans. Plasma Sci., 2004, 32(4):1544-1550.
[12] Laroussi M, Leipold F. Evaluation of the Roles of Reactive Species, Heat, and UV Radiation in the Inactivation of Bacterial Cells
by Air Plasmas at Atmospheric Pressure [J]. International Journal of Mass Spectrometry, 2004, 233(1-3): 81-86.
[13] Stoffels E, Aranda Gonzalvo Y, Whitmore T D, et al. Mass Spectrometric Detection of Short-Living Radicals Produced by a
Plasma Needle [J] .Plasma Sources Sci. Technol., 2007, 16(3), 549-556.
[14] Y.H.Luo,L.J.Yang, Y.P. Feng, et al .Study on the Technology of Air Sterilization by Discharge Plasmas[J]. High Voltage
Engineering, 2001, 27 (5): 39-40.
[15] Y.Zhang, X.A. Zeng, Q.B. Wen, et al. Effect of High Intensity Pulsed Electric Field on the Membrane Permeability and DNA of E
coli [J]. High Voltage Engineering, 2007, 33 (2): 90-93.
[16] H-U. Simon, A. Hay-Yehia, F. Levi-Schaffer. Role of Reactive Oxygen Species (ROS) in Apoptosis Induction [J]. Apoptosis, 2000,
5(5):415-418.
[17] I. E. Kieft, M. Kurdi, E. Stoffels. Reattachment and Apoptosis after Plasma-Needle Treatment of Cultured Cells [J]. IEEE Trans.
Plasma Sci., 2006, 34(4): 13311336.

1974-

1975-

2009

1999

2004 2008

2002 2010

E-mail:wuxili1984@163.com

E-mail: shixingmin142@163.com
1970-

1991 1994 2001

E-mail: gjzhang@mail.xjtu.edu.cn

-6www.ivypub.org/bf