You are on page 1of 1

LOMIBAO, Wilfredo B., 2008.

SUPERCRITICAL CARBON DIOXIDE


EXTRACTION AND MICROBIAL ASSAY OF ESSENTIAL OIL FROM
KALUMPANG (Sterculia foetida L ) SEEDS. (GT:MSCIED), 143 pp.

The essential oil of kalumpang ( Sterculia foetida L ) seeds was obtained by


supercritical carbon dioxide ( SC-CO2 ) extraction. The oil of air – dried
kalumpang seeds (300 grams) was extracted at a temperature of 40oC, a pressure
of 120 atm and at a constant flow rate of CO2 of 0. 280 m3/hr.
A statistical experimental design, the one – way ANOVA, was used to
investigate the antimicrobial properties of the seed extracts with microbiological
test against pathogenic strains of gram – positive (Staphylococcus aureus,
Bacillus subtilis), gram – negative (Escherichia coli and Pseudomonas
aeruginosa) and fungi (Aspergillus niger, Saccharomyces cerevisiae and Candida
albicans). The significant difference among the zone of inhibition was further
analyzed using Scheffe’s pairwise comparison of mean. The anti-microbial assay
of seedcoat and endosperm oil at certain concentrations revealed significant
susceptibility towards Bacillus subtilis and Aspergillus niger.
Likewise, the results confirmed that the respondents in Barangay Iponan,
Cagayan de Oro City lack the necessary information of the uses of kalumpang
plant especially the seeds as herbal medicine. However, this problem can be
remedied by educating the people through the conduct of seminar and distribution
of flyers. A lecture notes and powerpoint presentation was also integrated to
classroom lessons as a tool to facilitate the learning process.