REVISED 2013/HAL@KTT

BIO300 BIOLOGICAL TECHNIQUES AND SKILLS

Course Content: 1.0 Techniques in field investigation The sampling techniques Equipment used in field work The preservation and mounting techniques in plants The preservation and mounting techniques in animals 2.0 Techniques in Histology Cleaning and maintaining of slides and microscopes Fixative chemicals Staining procedures Slide preparations 3.0 Techniques in Microbiology Safety rules Sterilization of equipment Agar preparation Bacterial cultures 4.0 Techniques in Biochemical Analysis Extraction methods Chromatoghraphy techniques Spectrometry 5.0 Techniques in DNA Technology DNA Sequencing Polymerase Chain Reaction (PCR) Practicals 1. Techniques in Fields Investigation 2. Histology 3. Techniques in Microbiology I 4. Techniques in Microbiology II 5. Chromatography techniques 6. Spectrometry 7. Plasmid profiling and agarose gel electrophoresis Assessment : Course work Cognitive - Three tests Practical skills (Lab Reports) Mini Project (1) TOTAL

: 40% :30% :10% : 60% : 100%

BIO300

REVISED 2013/HAL@KTT

RECOMMENDED TEXT Jones, A., Reed, R. & Weyers, J. (2003). Practical Skill in Biology. Third Edition. Pearson Prentice Hall. REFERENCES 1. Roberts, M., King, T. and Reiss, M. (1994). Practical Biology for Advanced Level. Nelson International Edition. 2. Wan Ramlee, Sulaiman Shaari, Fauziah Ismail, Rusdin Laiman and Ismaniza Ismail. (2007). Guidelines on Supervision, Assessment, Evaluation and Format for Students' Project (Second Edition). University Publication Centre (UPENA), Universiti Teknologi MARA. 3. Skoog D.A. and Leary J.J. (1992). Principles of Instrumental Analysis. Fourth Edition. Saunders College Publication 4. Willard, Merit L.L. Jr and Dean J.A. (1995). Instrumental Methods of Analysis. Fourth Edition. N.Y. 5. Langford, A., Dean, J., Reed, R., Holmes, D., Weyers, J and Jones, A. (2005). Practical Skill in Forensic Science. Pearson Prentice Hall. 6. Henderson, P. A., Practical Methods in Ecology. (2003). Blackwell Publishing. PLEASE PREPARE: Log Book (Lab Report & Lab Progress) Your Mind & Soul

By: Miss Halimahtun Saediah bt Abu Bakar Biology lecturer Kolej Teknologi Timur Sepang

BIO300