(12) PATENT APPLICATION PUBLICATION (21) Application No.247 UDEL/2006 A. (a9) INDIA (22) Date of fiting of Applicatio 16/11/2006 (43) Publication Date : 2905/2008 (54) Titk of the invention : A NOVEL PROTEASE FOR INDUSTRIAL APPLICATIONS. (71)Name of Applicant = 1)COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH ‘Address of Applicant -ANUSANDHAN BHAWAN RAFT (61) International classification -A61K31/403] MARG, NEW DELHI-110001, INDIA Dethi India GI) Priority Document No 2)MACS-AGHARKAR RESEARCH INSTITUTE (G2) Priority Date 3)INDIAN INSTITUTE OF SCIENCE (33) Name of priority country (72)Name of Inventor = (86) International Application No SM Filing Date 2)VASUDEO PANDHARINATHL (87) International Publication No 3)PRADNYA PRALHAD KANEKAR (61) Patent of Addition to Application Number 4)PRASHANT KAMALAKAR DHAI ng Date 5)SEEMA SHREEPAD SARNAIK (62) Divisional to Application Number NARASIMHAN KANNAN CHANDRA BARU Filing Date 7)BADDIPUDI RA MANIAT. SRAMA RAJARAM 9yTHIRUMALACHARI RAMASAMI I) VEGNISETTIPALLT KRISHNATAIH SAIKUMARE 11)PADMANABITAN BALARAM. G7) Abstract The prevent Tavenion hates to an exraceHTar enzyme prowase obtained by growing Ue culture of Pcudomonas seruginosa MCM B27 isolated from vermicullure pit soil and deposited in MTCC, IMTECH, Chandigarh with designation MTCC 5210) in production medium of pH 7.0; containing soybean meal and tryptone as raw material, at 30°C for 72 h. The organism was also able to produce protease using different agricultural products’ byproducts as protein sources. The partially purified non- collagenolytic, calcium independent protease with molecular weight 60 kDa has eotivty in pH range of 6,0 11,0 and temperature range of25-65°C; stability in pH range of 6.0-10.0 and temperature 25-45°C. The protease activity was retained for 8 months when Sored of ambient temperature Arnmonium sulphate precipitated enzyme was able to completely dchair animal skins and hides without chemicals ike Hime, sium sufphide and calcium,