Area of PCM peak

32485.46
43789.7
69712.88
256257.14
320886.42
562925.52
992654.01

1200000
1000000

Area of PCM peak

Final concentration in
RLM (M)
1
2.5
5
10
25
50
100

800000

600000
400000
200000
0

Linearity of PCM

y = 9546.5x + 61639
R = 0.978

Linearity of
PCM
Linear
(Linearity of
PCM)

20

40

60

80

Concentration (M)

100

120

2mM Phenacetin with different concentrations of Arjuna Extract

Concentration of Arjuna
5ppm
10ppm
25ppm
50ppm
125ppm

A(Metabolite) 1
341905.04
325965.54
309268.26
313351.16
20676.82

A(Metabolite) 2
328358.24
318857.78
334644.09
310503.94
88677.15

Concentration of Arjuna
5ppm
10ppm
25ppm
50ppm
125ppm

Avg A(Metabolite)
335161.64
322411.66
321956.75
311927.55
54676.985

Avg A(PCM)
71534.325

71176.84
63968.21
49467.57
40986.01

6mM Phenacetin with different concentrations of Arjuna Extract

Concentration of Arjuna
5ppm
10ppm
25ppm
50ppm
125ppm

A(Metabolite) 1
380428.39
360586.83
333318.48
309074.55
269137.8

A(Metabolite) 2
374326.63
376739.23
392843.13
381406.3
186350.16

Concentration of Arjuna
5ppm
10ppm
25ppm
50ppm
125ppm

Avg A(Metabolite)
446085.8
399310.5
408474.8
185813.8
340564

Avg A(PCM)
73074.52
86881.27
77959.99
59386.65
48774.91

12mM Phenacetin with different concentrations of Arjuna Extract

Concentration of Arjuna
5ppm
10ppm
25ppm
50ppm
125ppm

A(Metabolite) 1
402151.7
364544.22
408399.67
326568.51
275332.22

A(Metabolite) 2
490019.83
434076.85
408549.86
45058.99
405795.75

Concentration of Arjuna
5ppm

Avg A(Metabolite)
446085.765

Avg A(PCM)
146090.935

10ppm
25ppm
50ppm
125ppm

3993310.535
408474.765
185813.75
340563.985

114129.315
111357.545
36638.415
38931.175

24mM Phenacetin with different concentrations of Arjuna Extract

Concentration of Arjuna
5ppm
10ppm
25ppm
50ppm
125ppm

A(Metabolite) 1
417753.24
427946.98
471242.02
382728.53
329137.94

A(Metabolite) 2
355258.91
384640.67
497016.94
349148.93
327428.58

Concentration of Arjuna
5ppm
10ppm
25ppm
50ppm
125ppm

Avg A(Metabolite)
386506.075
406293.825
484129.48
365938.73
328283.26

Avg A(PCM)
143622.945
169921.2
45223.99
36991.83
32980.925

48mM Phenacetin with different concentrations of Arjuna Extract

Concentration of Arjuna
5ppm
10ppm
25ppm
50ppm
125ppm

A(Metabolite) 1
508618.04
424393.67
436362.5
426073.46
133782.68

A(Metabolite) 2
460220.79
342130.2
381615.7
355593.03
272958.99

Concentration of Arjuna
5ppm
10ppm
25ppm
50ppm
125ppm

Avg A(Metabolite)
484419.41
383261.93
408989.1
390833.24
203370.83

Avg A(PCM)
240237.355
229284
151385.16
131797.11
12033.25

Negative Control
Concentration of Phenacetin
2mM
6mM
12mM
24mM

A(Metabolite)
294356.39
290929.19
327126.14
330638.73

A(PCM)
10231.32
37788.45
43542.75
55672.88

48mM

314451.93

109598.14

A(PCM) 1
72033.14
71836.79
63322.04
51110.45
40133.43

A(PCM) 2
71035.51
70516.89
64614.38
47824.69
41838.58

A(Phenacetin) 1
214425.89
312829.64
289311.49
343718.66
288674.09

A(Phenacetin) 2
274752.45
365534.87
319002.05
295440.21
360460.09

A(Caffeine) 1
637323.96
656109.91
716597.34
690920.2
680911.53

A(PCM) 2
77873.76
85370.68
79654.5
53481.11
49431.2

A(Phenacetin) 1
392169.55
577435.04
572277.95
455710.27
419313.78

A(Phenacetin) 2
368488.53
500766.62
526609.88
587627.87
475115.64

A(Caffeine) 1
586564.4
710185.89
702258.19
665934.83
704060.46

A(PCM) 2
156202.79
122090.43
132236.65
20197.66
29145.09

A(Phenacetin) 1
2022321.8
1208611.35
1833465.42
2771910.81
1967951.3

A(Phenacetin) 2
1532851.12
1708721.71
1632239.66
1837311.57
2407422.59

A(Caffeine) 1
574300.03
687320.27
650969.92
669389.74
649248.09

Avg A(Phenacetin)
244589.2
339182.3
304156.8
319579.4
324567.1

A(PCM) 1
73074.52
88391.86
76265.48
51292.18
48118.61
Avg A(Phenacetin)
380329
539100.8
549443.9
521669.1
447214.7

A(PCM) 1
135979.08
106168.2
90478.44
53079.17
28717.26
Avg A(Phenacetin)
1777586.46

1458667.53
1732853.54
2304611.19
2187687.95

A(PCM) 1
152071.57
190490.39
46566.1
36122.14
32486.56

A(PCM) 2
135174.32
149352.01
43881.88
37861.52
33475.29

A(Phenacetin) 1
2223971.07
2693992.23
3046779.33
2830240.81
2329533.68

A(Phenacetin) 2
1805143.61
1666138.66
3116203.47
2802846.56
2942204.66

A(Caffeine) 1
614749.36
705816.61
744320.61
639258.54
727887.17

A(PCM) 2
231409.43
201754.91
162308.53
139676.22
13386.1

A(Phenacetin) 1
4418048.93
7223662.6
7216377.59
5831158.9
7479017.96

A(Phenacetin) 2
3948483.01
6576618.62
5938606.63
6650279.73
3927444.92

A(Caffeine) 1
657163.4
657940.91
688582.05
665308.05
712072.32

Avg A(Phenacetin)
2014557.34
2180065.44
3081491.4
2816543.68
2635869.17

A(PCM) 1
249065.28
256813.09
140461.8
123918
10680.35
Avg A(Phenacetin)
4183265.97
6900140.61
6577492.11
6240719.31
5703231.44

A(Phenacetin)
8626.76
297284.22
404142.89
701385.42

A(Caffeine)
760314.61
727708.19
714409.9
706771.71

2856913.05

760352.36

A(Caffeine) 2
647857.04
659714.47
700653.32
665910.07
652154.97

A(Met 1) : A(Caff 1)
0.536469773
0.49681545
0.431578856
0.453527281
0.030366383

A(Met 2) : A(Caff 2)
0.506837496
0.483326946
0.477617219
0.466285095
0.13597558

A(PCM 1) : A(Caff 1)
0.113024371
0.109488957
0.088364883
0.073974462
0.058940741

A(Caffeine) 2
618132.89
728730.43
704194.41
686081.58
685412.23

A(Met 1) : A(Caff 1)
0.648570541
0.507735841
0.474638081
0.464121316
0.382265182

A(Met 2) : A(Caff 2)
0.605576303
0.516980236
0.557861756
0.555919749
0.271880413

A(PCM 1) : A(Caff 1)
0.124580558
0.124462991
0.108600343
0.077022822
0.068344429

A(Caffeine) 2
661258.98
646078.51
679496.15
670475.77
679072.77

A(Met 1) : A(Caff 1)
0.700246699
0.530384794
0.627371031
0.487860047
0.424078598

A(Met 2) : A(Caff 2)
0.741040719
0.671863935
0.60125412
0.067204502
0.597573291

A(PCM 1) : A(Caff 1)
0.23677359
0.154466854
0.138990201
0.079294866
0.044231566

A(Caffeine) 2
581680.43
711926.38
784036.85
679629.13
726362.86

A(Met 1) : A(Caff 1)
0.679550508
0.606314691
0.633116984
0.598706949
0.452182637

A(Met 2) : A(Caff 2)
0.610745852
0.540281525
0.633920383
0.513734498
0.450778252

A(PCM 1) : A(Caff 1)
0.247371661
0.269886522
0.062561884
0.056506308
0.044631313

A(Caffeine) 2
675720.58
635255.87
644589.32
690367.97
726359.69

A(Met 1) : A(Caff 1)
0.773959779
0.645033108
0.633711698
0.640415308
0.187877939

A(Met 2) : A(Caff 2)
0.681081506
0.538570702
0.592029201
0.515077532
0.375790388

A(PCM 1) : A(Caff 1)
0.379000535
0.390328502
0.203987019
0.186256577
0.014998968

A(PCM 2) : A(Caff 2)
0.109646891
0.106890016
0.092220187
0.071818542
0.064154353

A(PCM 2) : A(Caff 2)
0.12598223
0.117149877
0.11311436
0.077951532
0.072118935

A(PCM 2) : A(Caff 2)
0.236220293
0.188971508
0.194609859
0.03012437
0.04291895

A(PCM 2) : A(Caff 2)
0.232385882
0.209785751
0.055969155
0.055709089
0.046086181

A(PCM 2) : A(Caff 2)
0.342463197
0.317596294
0.251801457
0.202321408
0.018429024

A(Metabolite) 1
341905.04
325965.54
309268.26
313351.16
20676.82

A(Metabolite) 2 Avg
328358.24
318857.78
334644.09
310503.94
88677.15

335131.64
322411.66
321956.175
311927.55
54676.985

A(Metabolite) 1
402151.7
364544.22
408399.67
326568.51
275332.22

A(Metabolite) 2
490019.83
434076.85
408549.86
45058.99
405795.75

446085.765
399310.535
408474.765
185813.75
340563.985

A(Metabolite) 1
402151.7
364544.22
408399.67
326568.51
275332.22

A(Metabolite) 2
490019.83
434076.85
408549.86
45058.99
405795.75

446085.765
399310.535
408474.765
185813.75
340563.985

A(Metabolite) 1
417753.24
427946.98
471242.02
382728.53
329137.94

A(Metabolite) 2
355258.91
384640.67
497016.94
349148.93
327428.58

386506.075
406293.825
484129.48
365938.73
328283.26

A(Metabolite) 1
508618.04
424393.67
436362.5
426073.46
133782.68

A(Metabolite) 2
460220.79
342130.2
381615.7
355593.03
272958.99

484419.415
383261.935
408989.1
390833.245
203370.835

A(PCM) 1
61033.14
71836.79
63322.04
51110.45
40133.43

A(PCM) 2
63035.51
70516.89
64614.38
47824.69
41838.58

A(PCM) 1
73074.52
108391.86
76265.48
51292.18
48118.61

A(PCM) 2
73074.52
85370.68
79654.5
67481.11
49431.2

A(PCM) 1
135979.08
106168.2
90478.44
53079.17
28717.26

A(PCM) 2
156202.79
122090.43
132236.65
20197.66
49145.09

A(PCM) 1
152071.57
190490.39
46566.1
36122.14
32486.56

A(PCM) 2
135174.32
149352.01
43881.88
37861.52
33475.29

A(PCM) 1
249065.28
256813.09
140461.8
123918
10680.35

A(PCM) 2
201409.43
201754.91
162308.53
139676.22
13386.1

62034.33
71176.84
63968.21
49467.57
40986.01

A(Phenacetin) 1 A(Phenacetin) 2
214425.89
274752.45
312829.64
365534.87
289311.49
319002.05
343718.66
295440.21
288674.09
360460.09

244589.17
339182.255
304156.77
319579.435
324567.09

73074.52
96881.27
77959.99
59386.65
48774.91

A(Phenacetin) 1 A(Phenacetin) 2
392169.55
368488.53
577435.04
500766.62
572277.95
526609.88
455710.27
587627.87
419313.78
475115.64

380329.04
539100.83
549443.915
521669.07
447214.71

146090.9
114129.3
111357.5
36638.42
38931.18

A(Phenacetin) 1 A(Phenacetin) 2
2022321.8
1532851.12
1777586.46
1208611.35
1708721.71
1458666.53
1833465.42
1632239.66
1732852.54
2771910.81
1837311.57
2304611.19
1967951.3
2407422.59
2187686.945

143622.9
169921.2
45223.99
36991.83
32980.93

A(Phenacetin) 1 A(Phenacetin) 2
2223971.07
1805143.61
2014557.34
2693992.23
1666138.66
2180065.445
3046779.33
3116203.47
3081491.4
2830240.81
2802846.56
2816543.685
2329533.68
2942204.66
2635869.17

225237.4
229284
151385.2
131797.1
12033.23

A(Phenacetin) 1 A(Phenacetin) 2
4418048.93
3948483.01
4183265.97
7223662.6
6576618.62
6900140.61
7216377.59
5938606.63
6577492.11
5831158.9
6650279.73
6240719.315
7479017.96
3927444.92
5703231.44

Conc of Substrate Conc of Inhibitor (ppm)

0
5
5
10
10
2mM
25
25
50
50
125
125

A(PCM)
10231.32
72033.14
71035.51
71836.79
70516.89
63322.04
64614.38
51110.45
47824.69
40133.43
41838.58

A(Caffeine)
760314.61
637323.96
647857.04
656109.91
659714.47
716597.34
700653.32
690920.2
665910.07
680911.53
652154.97

Conc of Substrate Conc of Inhibitor (ppm)

0
5
5
10
10
6mM
25
25
50
50
125
125

A(PCM)
37788.45
73074.52
77873.76
88391.86
85370.68
76265.48
79654.5
51292.18
53481.11
48118.61
49431.2

A(Caffeine)
727708.19
586564.4
618132.89
710185.89
728730.43
702258.19
704194.41
665934.83
686081.58
704060.46
685412.23

Conc of Substrate Conc of Inhibitor (ppm)

0
5
5
10
10
12mM
25
25
50
50
125
125

A(PCM)
43542.75
135979.08
156202.79
106168.2
122090.43
90478.44
132236.65
53079.17
20197.66
28717.26
29145.09

A(Caffeine)
714409.9
574300.03
661258.98
687320.27
646078.51
650969.92
679496.15
669389.74
670475.77
649248.09
679072.77

Conc of Substrate Conc of Inhibitor (ppm)

0
5
5
10

A(PCM)
55672.88
152071.57
135174.32
190490.39

A(Caffeine)
706771.71
614749.36
581680.43
705816.61

24mM

10
25
25
50
50
125
125

Conc of Substrate Conc of Inhibitor (ppm)

0
5
5
10
10
48mM
25
25
50
50
125
125

149352.01
46566.1
43881.88
36122.14
37861.52
32486.56
33475.29

711926.38
744320.61
784036.85
639258.54
679629.13
727887.17
726362.86

A(PCM)
109598.14
249065.28
231409.43
256813.09
201754.91
140461.8
162308.53
123918
139676.22
10680.35
13386.1

A(Caffeine)
760352.36
657163.4
675720.58
657940.91
635255.87
688582.05
644589.32
665308.05
690367.97
712072.32
726359.69

Area Ratio Avg Area Ratio

0.013456693
0.01345669
0.113024371
0.109646891
0.11133563
0.109488957
0.106890016
0.1081895
0.088364883
0.092220187
0.09029255
0.073974462
0.071818542
0.0728965
0.058940741
0.064154353
0.06154755

Area Ratio Avg Area Ratio

0.051928026
0.05192803
0.124580558
0.12598223
0.125281395
0.124462991
0.117149877
0.120806435
0.108600343
0.11311436
0.11085735
0.077022822
0.077951532
0.077487175
0.068344429
0.072118935
0.07023168

Area Ratio Avg Area Ratio

0.060949253
0.06094925
0.23677359
0.236220293
0.2364969
0.154466854
0.188971508
0.17171918
0.138990201
0.194609859
0.16680003
0.079294866
0.03012437
0.05470962
0.044231566
0.04291895
0.04357526

Area Ratio Avg Area Ratio

0.078770668
0.07877067
0.247371661
0.232385882
0.23986152
0.269886522

0.209785751
0.062561884
0.055969155
0.056506308
0.055709089
0.044631313
0.046086181

0.23983613
0.05926552
0.0560386
0.04535874

Area Ratio Avg Area Ratio

0.144141251
0.14414125
0.379000535
0.342463197
0.360731865
0.390328502
0.317596294
0.35396239
0.203987019
0.251801457
0.22789424
0.186256577
0.202321408
0.19428899
0.014998968
0.018429024
0.01671399

Conc of Substrate

2mM

Conc of Substrate

6mM

Conc of Substrate

12mM

Conc of Substrate

24mM

Conc of Substrate

48mM

Conc of Inhibitor (ppm)

0
5
10
25
50
125

Avg Area Ratio/mL

0.01345669
0.11133563
0.1081895
0.09029255
0.0728965
0.06154755

Conc of Inhibitor (ppm)

0
5
10
25
50
125

Avg Area Ratio/mL

0.05192803
0.125281395
0.120806435
0.11085735
0.077487175
0.07023168

Conc of Inhibitor (ppm)

0
5
10
25
50
125

Avg Area Ratio/mL

0.06094925
0.2364969
0.17171918
0.16680003
0.05470962
0.04357526

Conc of Inhibitor (ppm)

0
5
10
25
50
125

Avg Area Ratio/mL

0.07877067
0.23986152
0.23983613
0.05926552
0.0560386
0.04535874

Conc of Inhibitor (ppm)

0
5
10
25
50
125

Avg Area Ratio/mL

0.14414125
0.33853334
0.35396239
0.22789424
0.19428899
0.01671399

Avg Area Ratio/100L

0.001345669
0.011133563
0.01081895
0.009029255
0.00728965
0.006154755

Avg Area Ratio/100L/min

1.49519E-05
0.000123706
0.000120211
0.000100325
8.09961E-05
6.83862E-05

Avg Area Ratio/100L/min/mg (v)

9.96792E-05
0.000824708
0.000801404
0.000668834
0.000539974
0.000455908

Avg Area Ratio/100L

0.005192803
0.01252814
0.012080644
0.011085735
0.007748718
0.007023168

Avg Area Ratio/100L/min

5.76978E-05
0.000139202
0.000134229
0.000123175
8.60969E-05
7.80352E-05

Avg Area Ratio/100L/min/mg (v)

0.000384652
0.00092801
0.000894862
0.000821166
0.000573979
0.000520235

Avg Area Ratio/100L

0.006094925
0.02364969
0.017171918
0.016680003
0.005470962
0.004357526

Avg Area Ratio/100L/min

6.77214E-05
0.000262774
0.000190799
0.000185333
6.07885E-05
4.8417E-05

Avg Area Ratio/100L/min/mg (v)

0.000451476
0.001751829
0.001271994
0.001235556
0.000405256
0.00032278

Avg Area Ratio/100L

0.007877067
0.023986152
0.023983613
0.005926552
0.00560386
0.004535874

Avg Area Ratio/100L/min

8.7523E-05
0.000266513
0.000266485
6.58506E-05
6.22651E-05
5.03986E-05

Avg Area Ratio/100L/min/mg (v)

0.000583486
0.001776752
0.001776564
0.000439004
0.000415101
0.000335991

Avg Area Ratio/100L

0.014414125
0.033853334
0.035396239
0.022789424
0.019428899
0.001671399

Avg Area Ratio/100L/min

0.000160157
0.000376148
0.000393292
0.000253216
0.000215877
1.85711E-05

Avg Area Ratio/100L/min/mg (v)

0.001067713
0.002507654
0.002621944
0.001688105
0.001439178
0.000123807

1/v
10032.18473
1212.549837
1247.810555
1495.139964
1851.940765
2193.426058

1/s
0.5
0.5
0.5
0.5
0.5
0.5

1/v
2599.752003
1077.574208
1117.49014
1217.78123
1742.223794
1922.209464

1/s
0.1666
0.1666
0.1666
0.1666
0.1666
0.1666

1/v
2214.957526
570.8320067
786.1672761
809.3523724
2467.573345
3098.088227

1/s
0.08333
0.08333
0.08333
0.08333
0.08333
0.08333

1/v
1713.835873
562.8247499
562.8843327
2277.884342
2409.053759
2976.27315

1/s
0.04166
0.04166
0.04166
0.04166
0.04166
0.04166

1/v
936.5813048
398.779039
381.3964529
592.3800444
694.8412259
8077.065979

1/s
0.020833
0.020833
0.020833
0.020833
0.020833
0.020833

Lineweaver Burk plot

Conc of Inhibitor

5 ppm

Conc of Inhibitor

10 ppm

Conc of Inhibitor

25 ppm

Conc of Inhibitor

50 ppm

Conc of Inhibitor

125 ppm

Conc of Substrate (mM)

2
6
12
24
48

1/v
1212.549837
1077.574208
570.8320067
562.8247499
398.779039

1/s
0.5
0.1666
0.08333
0.04166
0.020833

Conc of Substrate (mM)

2
6
12
24
48

1/v
1247.810555
1117.49014
786.1672761
562.8843327
381.3964529

1/s
0.5
0.1666
0.08333
0.04166
0.020833

Conc of Substrate (mM)

2
6
12
24
48

1/v
1495.139964
1217.78123
809.3523724
2277.884342
592.3800444

1/s
0.5
0.1666
0.08333
0.04166
0.020833

Conc of Substrate (mM)

2
6
12
24
48

1/v
1851.940765
1742.223794
2467.573345
2409.053759
694.8412259

1/s
0.5
0.1666
0.08333
0.04166
0.020833

Conc of Substrate (mM)

2
6
12
24

1/v
2193.426058
1922.209464
3098.088227
2976.27315

1/s
0.5
0.1666
0.08333
0.04166

48

8077.065979

0.020833

1/s
0.5
0.1666
0.08333
0.04166
0.020833

1/v
1212.549837
1077.574208
570.8320067
562.8247499
398.779039

5 ppm
1400
1200
1000
800
600
400
200
0
0

1/s
0.5
0.1666
0.08333
0.04166
0.020833

1/v
1247.810555
1117.49014
786.1672761
562.8843327
381.3964529

0.1

0.2

0.3

0.4

10 ppm
1600
1400
1200
1000
800
600
400
200
0
0

1/s
0.5
0.1666
0.08333
0.020833

1/v
1495.139964
1217.78123
809.3523724
592.3800444

0.1

0.2

0.3

0.4

25 ppm
1800
1600
1400
1200
1000
800
600
400
200

400
200
0
0

1/s
0.5
0.1666
0.08333
0.04166
0.020833

1/v
1851.940765
1742.223794
2467.573345
2409.053759
694.8412259

0.1

0.2

0.3

0.4

50 ppm
3000
2500
2000
1500
1000
500
0
0

1/s
0.5
0.1666
0.08333
0.04166
0.020833

1/v
2193.426058
1922.209464
3098.088227
2976.27315
8077.065979

0.1

0.2

0.3

0.4

125 ppm
9000
8000
7000
6000
5000
4000
3000
2000
1000
0
0

0.1

0.2

0.3

0.4

5 ppm

y = 1575.7x + 508.49

slope = 1575.7

5 ppm
Linear (5 ppm)

0.4

0.5

0.6

10 ppm

y = 1559.4x + 565.77

slope = 1559.4

10 ppm
Linear (10 ppm)

0.4

25 ppm

0.5

0.6

y = 1737.6x + 693.84

slope = 1737.6

25 ppm
Linear (25 ppm)

0.4

0.5

0.6

50 ppm

y = 306.14x + 1783.4

slope = 306.14
50 ppm
Linear (50 ppm)

0.4

0.5

0.6

125 ppm

y = -6462.1x + 4703.4

slope = -6462.1
125 ppm
Linear (125 ppm)

0.4

0.5

0.6

1/s
0.5
0.1666
0.08333
0.04166
0.020833

5ppm

10ppm

25ppm

1212.549837
1077.574208
570.8320067
562.8247499
398.779039

1247.810555
1117.49014
786.1672761
562.8843327
381.3964529

1495.139964
1217.78123
809.3523724
2277.884342
592.3800444

1400
y = 1575.7x + 508.49
1200

1000
800
600

400
200
0
0

0.1

0.2

0.3

0.4

0.5

508.4

3000

2000

[I] concentration

slope
5
10
25
50
125

1000

1575.7
1559.4
1737.6
306.14
-6462.1

0
-1000 0

-2000
-3000
-4000
-5000
-6000
-7000

[I] concentration

slope
5
10

1575.7
1559.4

2000
1800
1600
1400
1200

20

40

25
50

1737.6
306.14

1400
1200
1000
800
600
400
200
0
0

10

20

50ppm

125ppm

1851.940765
1742.223794
2467.573345
2409.053759
694.8412259

2193.426058
1922.209464
3098.088227
2976.27315

3500
3000
2500
Series1

2000

Linear (Series1)

1500
1000

500
0

0.6

0.1

0.2

0.3

0.4

0.001966955 vmax
3.097954367 Km

slope

60

80

100

y = -69.612x + 2736.7

120

140

slope

Linear (slope )

slope

y = -28.004x + 1924.8

Put x = 0, we get, y = 1924

now, slope = km/vmax

0.5

km = -28*0.0005197 = -0.0145516
slope
Linear (slope )

20

30

40

50

60

5ppm
10ppm
25ppm
50 ppm

125
Linear (5ppm)
Linear (10ppm)
Linear (25ppm)

Linear (50 ppm)

0.5

0.6

thus, vmax = 1/1924 = 0.0005197