DANGOOK UNIVERSITY

PROTOCOL

ANIMAL : ROSS 308 640 HEAD

PERIOD : 4WEEKS
GROUP

1) CON (BASAL DIET),

2) GM1 (CON + 0.025% GROMONE),
3) GM2 (CON + 0.05% GROMONE)
4) GM3 (CON + 0.1% GROMONE)
4GROUPS X 16 REPLICA, 16HEADS RANDOM

TEST FEED

3 FLOORS CAGE
AD LIBITUM : WATER & FEED

TEST FACTORS

WEIGHT GAIN : START, 1WEEK OLD, 4WEEK

OLD
FEED INTAKE
FEED EFFICIENCY
MEAT QUALITY : 6HEADS / GROUP,
WEIGHT OF LIVER, SPLEEN, GIZZARD,
FABRICIOUS, BREAST, ABDOMINAL FAT
WEIGHT AND LENGTH OF FEMUR


MEAT QUALITY : COLORIMETRY, MODEL CR-210,
JAPAN FOR BREAST MEAT OF 2 HEADS PER
GROUP
WATER HOLDING CAPACITY : HOFFMAN
METHOD, BODY SURFACE VS MUSCLE SURFACE
DRIP LOSS : 2.54CM THICKNESS IN
POLYETHYLENE BAG FOR 7DAYS ON 4C
pH : BREAST BY pH meter


BLOOD CHEMISTRY
6HEADS / GROUP AT 4WEEKS OLD, WING VEIN
RBC, WBC, LYMPHOCYTE
IgG, IGF-1

DIGESTION RATE
4WEEKS OLD FECES BY Cr2O3 0.2% before 5days

RESULT

Table 1. Effect of Gromone on meat quality in broilers1

Items
0 d BW, g
7 d BW, g
28 d BW, g
0-7 d
BWG, g
FI, g
FCR
8-28 d
BWG, g
FI, g
FCR
0-28 d
BWG, g
FI, g
FCR
1Abbreviation:

CON
40.68
417.05
1460.47

GM1
40.12
416.50
1508.22

GM2
40.10
423.92
1541.15

GM3
40.12
420.14
1468.69

0.19
10.38
25.61

376
557
1.481

377
550
1.459

387
560
1.447

380
561
1.476

11
15
0.051

1043b
1676
1.607

1092ab
1675
1.534

1117a
1689
1.512

1049ab
1679
1.601

22
22
0.032

1419b
2233
1.574

1469ab
2225
1.515

1504a
2249
1.495

1429ab
2240
1.568

24
33
0.028

SE2

CON, basal diet; GM1, basal diet + 0.25% Gromone; GM2, basal
diet + 0.05% Gromone; GM3, basal diet + 0.1% Gromone.
2 Standard error.
a,b Means in the same row with different superscripts differ (P < 0.05).

Table 2. Effect of Gromone on meat quality in broilers1

Items
CON
GM1
GM2
GM3
SE2
pH value
5.68
5.73
5.71
5.65
0.10
Breast muscle color
Lightness (L*)
55.52
55.44
56.08
56.43
1.47
Redness (a*)
15.36
15.88
14.76
15.11
0.58
Yellowness (b*)
14.52
15.49
14.83
15.04
0.62
WHC, %
61.86
62.18
61.37
62.44
1.54
Drip loss, %
1d
2.72
2.78
2.70
2.86
0.29
3d
4.86
5.08
4.79
4.60
0.25
5d
8.49
8.44
8.30
8.40
0.42
7d
11.38
11.31
11.26
11.43
0.43
Relative organ weight, %
Liver
2.97
3.28
3.50
3.17
0.20
Spleen
0.17
0.14
0.14
0.14
0.02
Bursa of fabricius
0.15
0.15
0.14
0.14
0.02
Breast muscle
17.39
17.36
17.05
16.23
0.63
Abdominal fat
1.99a
1.57b
1.44b
1.62ab
0.12
b
ab
a
ab
Gizzard
0.94
0.99
1.15
1.04
0.05
Femur length, cm
8.98
9.28
9.04
9.14
0.15
Femur weight, g
12.32b
13.58a
14.48a
14.18a
0.41
1Abbreviation: CON, basal diet; GM1, basal diet + 0.25% Gromone; GM2, basal diet +
0.05% Gromone; GM3, basal diet + 0.1% Gromone.
2 Standard error.
a,b Means in the same row with different superscripts differ (P < 0.05).

Table 3. Effect of gromax on blood profiles in broilers1

Items
WBC, 103/l

RBC, 106/l
Lymphocyte, %
IgG, mg/dL
IGF-1
1Abbreviation:

CON

301.43
1.945
79.75
1.45b
31.00b

GM1

304.90
1.978
83.50
1.55b
34.75a

GM2

307.15
2.01
80.75
1.71a
37.00a

GM3

309.00
2.03
81.75
1.58ab
35.25a

SE2

8.80
0.09
2.35
0.04
0.80

CON, basal diet; GM1, basal diet + 0.25% Gromone; GM2, basal
diet + 0.05% Gromone; GM3, basal diet + 0.1% Gromone.
2 Standard error.
a,b Means in the same row with different superscripts differ (P < 0.05).

Table 4. Effect of plant extracts on nutrient digestibility in broilers1

Items, %

CON

GM1

GM2

GM3

SE2

Dry matter

64.97

65.67

65.33

64.34

0.58

Nitrogen

63.89

65.43

68.43

67.05

1.78

Energy

66.54b

68.01ab

69.28a

68.12ab

0.65

1Abbreviation:

CON, basal diet; GM1, basal diet + 0.25% Gromone; GM2, basal diet +
0.05% Gromone; GM3, basal diet + 0.1% Gromone.
2 Standard error.
a,b Means in the same row with different superscripts differ (P < 0.05).