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  • PH P Log PH P When (HA) (A), or HA Is Half-Ionized

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    Charlie Mak
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    The document provides information on several key topics in biochemistry: 1) It discusses the Henderson-Hasselbalch equation which relates pH and the concentrations of acid and conjugate base forms of an acid. 2) It lists typical pKa values for common amino acid side chains and backbone groups. 3) It identifies several specific enzymes and chemicals that can be used to cleave peptides at particular amino acids, such as trypsin cleaving at arginine and lysine residues. 4) It provides skeletal structures of pyrimidine and purine nucleobases as well as Fischer and Haworth projections of common monosaccharides and disaccharides like glucose, fructose, and sucrose

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    The document provides information on several key topics in biochemistry: 1) It discusses the Henderson-Hasselbalch equation which relates pH and the concentrations of acid and conjugate base forms of an acid. 2) It lists typical pKa values for common amino acid side chains and backbone groups. 3) It identifies several specific enzymes and chemicals that can be used to cleave peptides at particular amino acids, such as trypsin cleaving at arginine and lysine residues. 4) It provides skeletal structures of pyrimidine and purine nucleobases as well as Fischer and Haworth projections of common monosaccharides and disaccharides like glucose, fructose, and sucrose

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    14 views1 page

    PH P Log PH P When (HA) (A), or HA Is Half-Ionized

    Uploaded by

    Charlie Mak
    The document provides information on several key topics in biochemistry: 1) It discusses the Henderson-Hasselbalch equation which relates pH and the concentrations of acid and conjugate base forms of an acid. 2) It lists typical pKa values for common amino acid side chains and backbone groups. 3) It identifies several specific enzymes and chemicals that can be used to cleave peptides at particular amino acids, such as trypsin cleaving at arginine and lysine residues. 4) It provides skeletal structures of pyrimidine and purine nucleobases as well as Fischer and Haworth projections of common monosaccharides and disaccharides like glucose, fructose, and sucrose

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    Appendix: CHEM2180 / Biochemistry

    HendersonHasselbalchequation

    conjugate base
    acid form
    [ ]
    pH p log
    [ ]
    pH p when [HA] [A ], or HA is half-ionized
    a
    a
    K
    K



    pK
    a
    forcommonaminoacids

    backbone -COOH: ~2, -NH


    3
    +
    : ~9
    sidechain Arg: 12.5, Asp: 3.9, Cys: 8.3, Glu: 4.3, His: 6.0,
    Lys: 10.5, Ser: ~13, Thr: ~13, Tyr: 10.1

    PolypeptideCleavagebyspecificenzymesandchemicals

    Trypsin : Arg-C, Lys-C


    Chymotrypsin : Phe-C, Trp-C, Tyr-C; Leu-C
    Clostripain : Arg-C
    Staphylococcal protease : Asp-C, Glu-C
    CNBr : Met-C

    *trypsin and chymotrypsin will not cleave peptide bonds
    where Pro is the amino acid contributing the N-atom


    Nucleobaseskeletons

    1
    3
    2
    4
    6
    5
    N
    N

    6
    7
    4
    5
    8
    2
    1
    9
    3
    N
    N
    H
    N
    N

    pyrimidine purine


    SimpleMichaelisMentenkinetics


    1 2 max
    m
    1 m
    [S]
    ;
    [S]
    k k V
    K v
    k K


    Fischerprojectionsofsomecommonmonosaccharides

    CHO
    CH
    2
    OH
    H OH
    H OH
    H OH

    CHO
    CH
    2
    OH
    H OH
    O H H
    H OH
    H OH
    CHO
    CH
    2
    OH
    O H H
    O H H
    H OH
    H OH

    CHO
    CH
    2
    OH
    H OH
    O H H
    O H H
    H OH

    CH
    2
    OH
    CH
    2
    OH
    C O
    O H H
    H OH
    H OH

    D-Ribose (Rib) D-Glucose (Glc) D-Mannose (Man) D-Galactose (Gal) D-Fructose

    Haworthprojectionsofsomecommondisaccharides




    Sucrose
    (glucose-1-2-fructose)
    Maltose
    (glucose--1,4-glucose)
    Cellobiose
    (glucose--1,4-glucose)

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