BIO I: Biochemistry Lab

Name Date Prediction: Predict which substances tested contain the following: A. Sugars: B. Starches: . Li!ids: D. Proteins Materials: corn syru!" gelatin solution" oli#e oil" regular soft drin$" diet soft drin$" !otato" a!!le %uice" distilled water" test tubes" test tube rac$s" hot !lates" brown !a!er bags" Biuret reagent" Benedict&s solution" iodine solution" bea$ers" test tube clam!s" dro!!ers" boiling chi!s" china mar$ers" safety glasses Testing: A. 'onosaccharides ( Disaccharides: ). Add * mL of each substance to be tested into indi#idual test tubes" label each tube with a china mar$er. *. Add * mL of Benedict&s solution+ swirl the test tubes to mi, the contents+ and !lace the tubes into a boiling water bath for - . / minutes. In the !resence of sugar+ Benedict&s solution changes from its initial blue to a green+ yellow+ orange or red color+ de!ending on the amount of sugar !resent. 0ecord your results as !ositi#e or negati#e 1may use 2slightly3 in addition for clarification if needed.4 B. Starches: A. Add * ml of each substance to be tested into indi#idual test tubes: label each tube with a china mar$er. B. Add )5 dro!s of iodine solution+ and swirl the test tubes to mi, the contents. In the !resence of starch+ iodine changes from brown6orange to a dar$ blue or #ery dar$ !ur!le color. . Proteins: ). Add * mL of each of the substances to be tested into indi#idual test tubes" label each tube with a china mar$er. *. Add * mL of Biuret reagent+ swirl the test tubes to mi, the contents+ and note any color change. If !rotein is !resent+ Biuret reagent changes from blue to #iolet or !ur!le. A !in$ish color indicates some amino acids may be !resent. 0ecord your results. D. Li!ids: ). Add a dro!!erful of each substance to se!arate !ieces of brown !a!er. Let the s!ots dry for a / . )5 minutes. 7old the !a!er to the light+ and chec$ for a translucent s!ot. Li!ids will lea#e a translucent s!ot on the !a!er+ while non6fat substances will dry without lea#ing a s!ot. 0ecord your results.

Data Table:

Substance Benedict&s color Sugars !resent9 Iodine color Starch !resent9 Biuret color Proteins !resent9 Brown !a!er Li!ids !resent9

orn syru! 8gg white Oli#e oil Soft Diet soft drin$ drin$

Potato

A!!le Distilled water