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Labeled Immunoassays
Designed for Ags and Abs that DO NOT react in precipitation or agglutination tests due to their small size or low concentrations.
Indirect method of detection:
Radioimmunoassay (RIA)
Competitive binding assay
Radioimmunoassay
Two Types
Number 1
Radioimmunoassay
Number 2
Enzyme Immunoassay
Immunoassay labels
Enzymes
Cheap Readily available Long shelf life Easily adapted to automation
Enzyme Immunoassay
Enzymes are naturally occurring molecules that catalyze specific biochemical reactions.
They react with suitable substances to produce products that are chromogenic (color), fluorogenic, or luminescent.
Fluorescent Immunoassay
Similar to ELISA but a fluorochrome is used rather than an enzyme.
Fluorochromes have the ability to absorb energy from light an emit it at a longer wavelength.
Chemiluminescent Immunoassays
Chemiluminescence is the production of light energy due to a chemical reaction.
Certain substances when oxidized can give off short or long bursts of light energy.