csveanancrat xnunrouocy 45, 259-273 (1978) Ascaris suum: Free Amino Acids and Proteins in the Pseudocoelom, Seminal Vesicle, and Glandular Vas Deferens M, Annas ! AND W. E, Foon Department of Bolngy, Wane Slate Unversty, Det, Michigan 48202, USA (Accept for puleton 3 May 1978) ‘Anas, M, aso Foon, W.E. 1978 Ascaris sum: Fee amino aie and protein in the pecdoteo, sennal vs, ad glider va deren, Experimental Prstabay 45, 60-273. The fee amino sce ad paeine of the seminal vse ad preadcoroe bis ithe male Aarts sum were examined sod compared. The svi Bd ctl ‘Chih concentration of sine he: alti ai ati of 6:1) whe the meewlocreonde wil contaoed noe gti ald an ine with nine, sen, eine, and proline bins the most abundant fee amin sei. The rota pee Inthe ena el difred from thors tn the peealorsonie fold ix bath tuber and moleelar weight. The sperm sctvating valtance (S83) proent fn Inogeats of the lanlr ar deletes of tale tomar i nondalrabls, besten, and can i predpttel vsing 45% tated Tamra nite Active mois can be recovered folowine pamgs ofthe terion Salas precipitates theagh trates membranes or by appli snd boson fo ‘fon exchange calarn. When rabfated fo SDS-papacyamide gel lectrophore, the ttre faci rveled both low and high wokecoer weight rites, Deng tongs to puny a inde stating sane tan noted thatthe mire heterogeneous ‘ontuned the igh stting cpacky. Thay no pre relatos between the ba Teel etry and the purty ofthe vars fos as dete, Tsoce Drscurons: Avert swum; Nematode partic; Amino acd, foe Pte Sina ld; Paendocosamic Mls Chandar vas defres, Sperm activating sultant (Sas).7 Gelfand Tissue ATi Re 8,275.28 19m Research (5b) Spenser Vera 1979 In vitro Activation and Behavior of the Ameboid Sperm of Ascaris suum (Nematoda)* M. Abbas and G.D. Cain Depart fZolog, Unverity of fw om Gy, Jowe, USA. ‘Summary. A system is described forthe study of activation and motility of ‘Ascaris spermatozoa in vito, Ativation was accomplished by addition ofthe ‘Sperm-actvating substances (SAS), extracted from the male accessory gnd, 10 fall incubated in phosphate-bulfered saline (pH7.4) at 37-39°C under fnterobie conditions (987N;, 8%CO,)- Activation is characterized by a change [fom spherical to ameboid shape with coalescence of the refringent ‘ranules, Thenormal ameboid spermatozoa bea several stubby and needle-like Flopodia at the lamelipodial margin. Within the amelipodium are bundles of ricrofilament-ike srdctures extending toward the pseudopodial membrane ‘and concenratng within the needle-like Slopodia. These filopodia exhibit a pendulous, sweeping motion with subsequent retraction and disappearence ‘thin the main lamelpodium. Membranes ofthe ameboidexllsinteract tthe scudopodial regions with patil fusion, a suggested by apparent membrane breakdown between interdigitating portions ofthe pseudopedia. Activation is complet in $1Smin is totally inhibited at 4°C andjor by an atmospheric vironment, but can be reinitated by transfer to anaerobic conditions at 22- 58°C Astivation ls requires favorable pH (6-8.7) and continual exposure to ‘ulcieatly high sodium concentrations (34-1544mM), lowering of sodium ‘concentration to 10mM causes ireversible inactivation. Sodium may be ‘epaced by potasium or ithium but nat by Tris or sucrose. Proteinases (COygimt) can act as activators eventhough SAS lack detectable proteolytic ‘activity aginst azoalbumin, zocasein, TAME and BTEE and SAS activation ‘was not inhibited by TLCK or soybean trypsin inhibitor. ‘Key words: scare sam ~ Nematoda ~ Spermatozoa ~ Activation ~ in vir, Saletan Ds 2, Ue om ow i. 1A 28, + Adu Acar um wer provided hoa ees of Wil abd Campa, Cla Rai, lon USA Th say nu ppv y pt user STO HOOD ad pode flowy PIRALos@ tom be Neste of Hel USA. m7.766/705090010079'502Celland Tissue Te Re (181 206553567 at Surface Receptors: Are They Involved in Transformation of Spermatozoa of Ascaris?* MAK. Abbas and G.D. Cain Deparment of Zale, Unt flow, foe iy own, USA ‘Summary. Exposure of the spheroidal spermatozoa of Ascaris sum to an extract ofthe male acessory sland causes ther transformation into meboid fall We ave investigated the mechanism ofthis eansformation, also termed “stvation, by labeling the proteins ofacessry land extract with Muorescein isothiocyanate (FITC) or [I], followed by qualitative localization of the Sperm activating substances (SAS) and quantitative measurements of [ "Tp SAS binding Fluorescent patches of FITC-conjugated SAS were localized at the spermatoroan surface and were concentrated primarily atthe posterior region. Few fluorescent patches were detectable in the region of the newly formed prewdopodia folowing wansformation. Although spermatozoan transfor tation oocure within -Smin aller exposore to SAS, the Muorescent patches became more distinc aera mininum ofS min and reached maximum density at 1S-20min Spermatozoa activated with ['T}SAS became radioactively labeled in Airset proportion fo the amount of available ['1]-SAS until saration level ‘was reached. SDS-polyacrykimide el eletrophoress combined with auto- fadiography indleated thatthe ell bind two SAS components, of sm {0,000 MW) and large (56,000 MW) sizes. These same two components were alo detectable ina membrane fraction, obtained by dllerenial centifugation, ‘ofthe spermatozoa alter incubation with [""T}SAS, Binding ofthe two SAS components was not inhibited by peeinevbation of the spermatozoa with trypsin or Concanavalin A: however, dhe 36,000 MW Seater: MX, At, Depress Tom, * Ad Acris ere oy Wn Copy, sla ap own, USA. Thi tay as ‘prairie tuo aie Hen US retly stand caeFATTY ACIDS OF THE AMEBOID SPERM OF ASCARIS SUUM (NEMATODA) MK. Ate, W. J Jon and 6. D.Can Diprnet Zap, Usb on, ows Cy, IA SDD, USA Arc, Toph wr xc th ance perm of Auris ma date nto Son pS eH hos ac a cote comatose TU "Fhe hy so smote of tna a tried ty wes homopuni GLC) J sen sa 1, M2, 2 ve soared (6-1, 18-2 201 20 OT ME aly te er mized ety ahem ayy SAE Seg ea eed eee esa esUltrastructure and Fatty Acid Composition of Fatty Acid-modified Morris 7777 Hepatoma Cells’ onammea ABB, TaJune Yo! and Joseph ies? sna fene 0, ne (62, asancone GO) ‘ee wee creosote garam ray Sees eer sae eeeteceseteereemnans ee eae en a amEffect of Fatty Acid Modification of Cultured Hepatoma Cells on Susceptibility to Natural Killer Cells" sees ns turk Speco, ere M. Denning, Rech Foy, Sephen Wher, Soe nw mayan, Sites eery amare {irr oe ane ais? =< 0.0 Pocrana nwa tay own Meee ace toler sactoay oe msae ofa