MAKALAH

Enzim Cyclodextrin Glucanotransferase (CGTase) dan

Pemanfaatannya dalam Teknoloi Panan
Disusun untuk memenuhi tugas Mata Kuliah Kapita Selekta Kimia yang dibimbing oleh Ibu Dra.
Anna Roosdiana, M.App.Sc
Oleh :
Nisfa Rohmatul L. 115090201111015
Yeni Dwi Lestari 115090201111017
Aulya i!iana 115090207111011
". #a$ar %i&a'sono 11509020711101(
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'AK"LTA$ MATEMAT&KA (A% &LM" PE%GETAH"A% ALAM
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/1/ Latar *elakan
)n*im a!alah +olon+an ,rotein yan+ !isintesis oleh sel hi!u, !an mem,unyai fun+si
,entin+ se-a+ai 'atalisator !alam setia, rea'si meta-olisme yan+ ter$a!i ,a!a or+anisasi hi!u,.
)n*im $u+a meru,a'an -io'atalisator yan+ menun$an+ -er-a+ai ,roses in!ustri. .al ini
!ise-a-'an en*im mem,unyai efisiensi !an efe'tifitas yan+ tin++i/ rea'sinya ti!a' menim-ul'an
,ro!u' sam,in+/ serta !a,at !i+una'an -erulan+'ali !en+an te'ni' amo-ilisasi 0Lehnin+er/
19951.
)n*im men$a!i ,rima!ona in!ustri saat ini !an !i masa yan+ a'an !atan+ 'arena melalui
,en++unaannya/ ener+i !a,at !ihemat !an ramah terha!a, lin+'un+an. 2aat ini ,en++unaan
en*im !alam in!ustri ma'anan !an minuman/ in!ustri te'stil/ in!ustri 'ulit !an 'ertas !i
3n!onesia sema'in hari sema'in menin+'at 0.ouston/ 19951.
)n*im -er,eran san+at ,entin+ !alam in!ustri ,an+an/ -ai' ,ro!u' ,an+an tra!isional
mau,un mau,un !esain ,ro!u' ,an+an yan+ -aru. 4en++unaan en*im !alam in!ustri ,an+an
!ila'u'an 'arena en*im meru,a'an alat yan+ i!eal !i+una'an untu' memani,ulasi -ahan5-ahan
-iolo+is. 6e-era,a 'euntun+an ,en++unaan en*im !alam ,en+olahan ,an+an a!alah aman
terha!a, 'esehatan 'arena -ahan alami/ men+'atalisis rea'si yan+ san+at s,esifi' tan,a efe'
sam,in+/ a'tif ,a!a 'onsentrasi yan+ ren!ah/ !a,at !iina'ti7asi/ !an !a,at !i+una'an se-a+ai
in!i'ator 'esesuaian ,roses ,en+olahan. %alau,un !emi'ian/ !ari ri-uan en*im yan+ !itemu'an
oleh ,ara ahli -io'imia/ hanya se-a+ian 'e&il en*im yan+ !a,at !imanfaat'an !alam in!ustri
,an+an04oe!$ia!i/ 20081.
9enis en*im yan+ !i,er+una'an untu' in!ustri ,an+an se-a+ian -esar yan+ ter+olon+
!alam hi!rolase/ se!an+'an en*im yan+ ter+olon+ !alam o'si!ore!u'tase -iasanya !i,er+una'an
untu' ,en+en!alian mutu ,ro!u' ,an+an. A!a 1( $enis en*im yan+ !i-uat se&ara in!ustri/
!iantaranya a!alah alfa5amilase/ &y&lo!e:trin +lu&anotransferase/ -eta5amilase/ -eta5+lu'anase/
selulase/ !e'stranase/ ,e'tinase/ +lu'o5amilase/ ,rotease/ rennet/ +lu'osa isomerase/ ,ullulanase/
!an -romelin02tryer/20001.
Dalam ma'alah ini a'an !i-ahas men+enai ,eran en*im &y&lo!e:trin +lu&anotransferase
!an ,emanfaatannya !alam -i!an+ te'nolo+i ,an+an 'hususnya !alam mem,ro!u'si
si'lo!e'strin.
/1- #umusan Masala2
Rumusan masalah yan+ a'an !i-ahas a!alah :
1. 6a+aimana 'ara'teristi' en*im &y&lo!e:trin +lu&anotransferase;
2. 6a+aimana en*im &y&lo!e:trin +lu&anotransferase !i,ro!u'si;
(. 6a+aimana me'anisme rea'si 'ataliti' en*im &y&lo!e:trin +lu&anotransferase;
<. 6a+aimana ,eran en*im &y&lo!e:trin +lu&anotransferase !alam te'nolo+i ,an+an;
/13 Tu4uan
1. "en+etahui 'ara'teristi' en*im &y&lo!e:trin +lu&anotransferase.
2. "en+etahui &ara mem,ro!u'si en*im &y&lo!e:trin +lu&anotransferase.
(. "en+etahui $enis5$enis rea'si yan+ !i'atalisis oleh en*im &y&lo!e:trin +lu&anotransferase.
<. "en+etahui ,eran en*im &y&lo!e:trin +lu&anotransferase !alam te'nolo+i ,an+an.
*A* &&
T&%!A"A% P"$TAKA
-1/ Penertian Enzim
)n*im meru,a'an mole'ul or+ani' yan+ 'om,le's !an ter!a,at !alam sel hi!u,/ yan+
!i!alamnya en*im -e'er$a se-a+ai 'atalisator untu' menim-ul'an ,eru-ahan 'imia ,a!a
-er-a+ai ma&am su-stansi. =atalisator -e'er$a mem,er&e,at rea'si 'imia. %alau,un 'atalisator
i'ut serta !alam rea'si/ ia 'em-ali 'e 'ea!aan semula -ila rea'si telah selesai. =emam,uan
en*im yan+ uni' !alam mela'sana'an trans,ortasinya yan+ 'has 'eti'a !iisolasi ini telah
menin+'at'an ,en++unaan en*im !alam -er-a+ai ,roses in!ustri0Lehnin+er/ 19951.
-1- Cara Ker4a Enzim $ecara "mum
)n*im -e'er$a !en+an men+uran+i ener+i a'ti7asi !ari su-strat tertentu. "e'anisme 'er$a
en*im yaitu !en+an teri'at sementara 'e su-strat untu' mem-entu' se-uah 'om,le's en*im5
su-strat yan+ le-ih ti!a' sta-il !i-an!in+ su-strat $i'a -er!iri sen!iri. 3ni menye-a-'an su-strat
mu!ah -erea'si. Den+an !emi'ian su-strat tere'sitasi 'e tin+'at ener+i le-ih ren!ah !en+an
mem-entu' ,ro!u'5,ro!u' rea'si yan+ -aru. 2elama -erlan+sun+nya rea'si/ en*im !ile,as'an
!alam 'ea!aan ti!a' -eru-ah. 4ele,asan en*im teta, utuh sehin++a -isa terus -erea'si !an
menye-a-'an en*im teta, efe'tif mes'i !alam $umlah yan+ san+at 'e&il 0Dosan$h/ N.2./ !an
=aur/ 9/ 20021.
-13 'aktor5'aktor yan Mem6enaru2i Ker4a Enzim
=e+iatan en*im !a,at -erlan+sun+ !en+an -ai' $i'a 'on!isi lin+'un+annya men!u'un+.
.al5hal yan+ mem,en+aruhi 'er$a en*im antara lain0La+a/ 200>1:
11 ?em,eratur
?em,eratur ren!ah yan+ men!e'ati titi' -e'u -iasanya ti!a' merusa' en*im. 4a!a
tem,eratur !imana en*im masih a'tif/ 'enai'an tem,eratur se-anya' 10
o
@/ menye-a-'an
'ea'tifan men$a!i 2 'ali le-ih -esar 0A10B21. 4a!a tem,eratur o,timum rea'si -erlan+sun+
,alin+ &e,at. 6ila tem,eratur !inai'an terus/ ma'a $umlah en*im yan+ a'tif a'an -er'uran+
'arena men+alami !enaturasi. 2e-a+ian -esar en*im men$a!i ti!a' a'tif ,a!a ,emanasan sam,ai
80
o
@. 3ni !ise-a-'an 'arena ,roses !enaturasi en*im.
21 ,.
6ila a'ti7itas en*im !iu'ur ,a!a ,. yan+ -erlainan/ ma'a se-a+ian -esar en*im a'an
menun$u'an a'ti7itas o,timum antara ,. 5/059/0. 3ni !ise-a-'an oleh/ ,a!a ,. ren!ah atau
tin++i/ en*im a'an men+alami !enaturasi/ selain itu en*im mau,un su-strat $u+a !a,at
men+alami ,eru-ahan muatan listri' !en+an a'i-at ,eru-ahan a'ti7itas en*im.
(1 =onsentrasi en*im !an 'onsentrasi su-strat
=e&e,atan re'asi en*im 071 -er-an!in+ lurus !en+an 'onsentrasi en*im C)n*D. "a'in
-esar $umlah en*im ma'in &e,at rea'sinya. 2elain itu/ -ila 'onsentrasi su-strat 021 -ertam-ah/
se!an+'an 'ea!aan lainya teta, sama/ 'e&e,atan rea'si $u+a a'an menin+'at sam,ai suatu -atas
ma'simum .
<1 9an+'a wa'tu
9an+'a wa'tu $u+a meme+an+ ,eranan ,entin+ terha!a, 'e+iatan en*im/ ma'in lama
en*im -erea'si !alam suatu -ahan ma'in -anya' ,ro!u'si yan+ !a,at !i&a,ai/ sehin++a en*im
a'an terus -e'er$a selama a!a -ahan tem,at en*im terse-ut -erea'si.
51 #a'tor5fa'tor lain
)n*im !a,at !irusa' !en+an ,en+o&o'an/ ,enyinaran ultra7iolet !an sinar5:/ sinar5E !an
sinar5F. Gntu' se-a+ian ini !ise-a-'an 'arena o:i!asi oleh ,ero'si!a yan+ !i-entu' ,a!a
,enyinaran terse-ut. =er$a en*im $u+a !i,en+aruhi oleh a!anya inhi-itor se,erti o-at5o-atan !an
se-a+ainya.
-10 $iklodekstrin
2i'lo!e'strin -iasa !ise-ut $u+a !en+an &y&loamylose yan+ meru,a'an 'om,onen yan+
!i-uat !ari mole'ul +ula yan+ salin+ teri'at ,a!a suatu &in&in 0&in&in oli+osa'ari!a1 !an
ter-entu' melalui ,roses si'lisasi oleh a'ti7itas @H?ase. 2tru'tur &y&lo!e:trin -er-entu' !onat
se,erti +am-ar -eri'ut 0.amilton !an #or+arty/ 20001 :
Ham-ar 1. 2tru'tur 2i'lo!e'strin
@y&lo!e:trin memili'i ,ermu'aan luar yan+ -ersifat hi!rofili' se!an+'an -a+ian !alam
ron++a5nya -ersifat non ,olar. A!anya -entu' terse-ut men+a'i-at'an &y&lo!e:trin !a,at
!i+una'an se-a+ai 'om,le's ,en+in'lusi !en+an senyawa lain. @y&lo!e:trin memili'i sifat yan+
uni'/ sehin++a -anya' !i+una'an !alam -er-a+ai in!ustri antara lain ,a!a in!ustri farmasi/
'osmeti'a/ ma'anan/ fla7our/ ,ertanian !an 'imia 0La+a/ 200>1. 6er!asar'an $umlah +lu'osa
yan+ menyusunnya/ &y&lo!e:trin !i-e!a'an atas 5&y&lo!e:trin 08 unit +lu'osa1/ 5&y&lo!e:trin
07 unit +lu'osa1 !an 5&y&lo!e:trin 0> unit +lu'osa1 se,erti ,a!a +am-ar -eri'ut :
Ham-ar 2. 9enis5$enis 2i'lo!e'strin
*A* &&&
PEM*AHA$A%
31/ Karakteristik Enzim Cyclodextrin Glucanotransferase
@H?ase meru,a'an en*im multifun+sional yan+ men+'atalisis ,em-entu'an
&y&lo!e:trin !ari ,ati !en+an rea'si intramole'ular 0si'lisasi1. @H?ase meru,a'an famili
yan+ sama !en+an alfa5amilase yan+ -iasa !ise-ut family 1( +li'osil5hi!rolase. 6er-a+ai
-a'teri yan+ telah !itentu'an se-a+ai ,ro!usen @H?ase a!alah aero-i' -a'teri mesofili'/
termofili' aero-i'/ termofili' anaero-i' !an aero-i' -a'teri al'alofili' 0?on'o7a/ 199>1.
Ham-ar (. )n*im @H?ase
6e-era,a s,esies -a'teri terutama Paenibacillus macerans 0!ahulu Bacillus
macerans1/ Bacillus circulans/ Bacillus megaterium/ Klebsiella sp serta s,e&ies Bacillus
al'alofili' !i'etahui se-a+ai ,en+hasil en*im @H?ase yan+ ,otensial. 6eri'ut a!alah ta-el
sifat5sifat -a'teri ,en+hasil @H?ase 0?on'o7a/ 199>1 :
Ham-ar <. 6a'teri 4en+hasil @H?ase
31- Produksi Cyclodextrin Glucanotransferase (CGTase)
)n*im @H?ases meru,a'an monomer yan+ !a,at -erasal !ari -a'teri 6a&illus s,/
"i&ro&o&&us s,/ 6. ma&erans/ 6. li&heniformis/ !an 6a&illus &ir&ulans. )n*im @H?ases
!a,at !i,eroleh !en+an &ara fermentasi ,a!a me!ia 'ultur yan+ ter!iri !ari 10 + te,un+
yan+ larut / 10 + ,e,ton 'e!elai / 5 + e'stra' ra+i / 1 + =
2
.4O
<
/ 0/2 + "+2O
<
I 7.
2
O !an
> + Na
2
@O
(
!en+an !iin'u-asi ,a!a suhu (7 J @ selama 98 $am !en+an terus menerus
!ia!u' !en+an 'e&e,atan 200 r,m. Gntu' memisah'an su,ernatan !en+an en!a,an yan+
-eru,a sel !i+una'an ,roses sentrifu+asi. 2u,ernatan yan+ !ihasil'an 'emu!ian
!imurni'an !en+an &ara filtrasi +el 0 'romato+arfi 'olom 1 !an ele'troforesis !en+an 2D2
4AH) yan+ !a,at men+hilan+'an massa mole'ul ,rotein ren!ah/ men+hasil'an
,enin+'atan a'ti7itas s,esifi' en*im @H?ase !an men+hasil'an !ua iso*im @H?ase
02un/201(1. 4ro!u'si @H?ase !a,at !io,timal'an !en+an memani,ulasi fa'tor5fa'tor
lin+'un+an se,erti 'onsentrasi nutrient !an 'om,osisi !ari me!ia ,ro!u'si 0 Noor/ 20111.
313 Mekanisme #eaksi Katalitik Enzim CGTase
)n*im @H?ase !a,at se&ara simultan men+'atalisis -e-era,a $enis rea'si/ ya'ni
rea'si si'lisasi !an rea'si non5si'lisasi. )n*im @H?ase mam,u men+'atalisis em,at $enis
rea'si : si'lisasi/ coupling/ !is,ro,orsionasi/ !an hi!rolisis. =eem,at $enis rea'si terse-ut
mem,unyai 'emiri,an me'anisme 'ataliti' yan+ umum ter$a!i ,a!a se-a+ian -esar en*im
+ly&osyl5hy!rolase !an rea'sinya a!alah se-a+ai -eri'ut 0Noor/ 20111 :
HL@
n
HL@
0n5:1
K @H
:
0 rea'si 'e'anan meru,a'an rea'si si'lisasi !an
rea'si 'e'iri a!alah rea'si &ou,lin+1
HL@
m
K HL@
n
HL@
0mK:1
K HL@
0 n5:1
0 rea'si !is,ro,orsionasi1
=eteran+an :
@H: : si'lo!e'strin 0 :B 8/7/>1
HL@n !an HL@m : Rantai L51/<5D+lu'o,iranosil
n !an m : Resi!u +lu'osa
2i'lisasi meru,a'an ,roses ,emutusan rantai ,olisa'ari!a linear 'emu!ian 'e!ua
fra+men u$un+nya !i+a-un+ untu' mem-entu' !e'strin sir'uler 0@y&lo!e:trin : @D1
!en+an $umlah !asar +ula resi!u tertentu. ?er!a,at ti+a ti,e si'lo!e'strin yan+ !i-e!a'an
menurut $umlah +ula resi!unya : L5@D !en+an 8 resi!u/ E5@D !en+an 7 resi!u/ !an F5@D
!en+an > resi!u. Rea'si @ou,lin+ meru,a'an rea'si 'e-ali'an !ari ,roses si'lisasi/ yaitu
rea'si 'eti'a en*im @H?ase mem-u'a &in&in si'lo!e'strin untu' mem-entu' !e'strin
linear yan+ 'emu!ian -eri'atan !en+an oli+osa'ari!a linear.
2e!an+'an rea'si !is,ro,orsionasi ham,ir miri, !en+an rea'si &ou,lin+/ teta,i
o-$e' ,em-elahannya -eru,a oli+osa'ari!a linear yan+ selan$utnya a'an -eri'atan !en+an
oli+osa'ari!a lainnya. Rea'si ini -erlan+sun+ melalui me'anisme rea'si trans+li'osilasi.
)n*im @H?ase $u+a mem,unyai a'ti7itas men+hi!rolisis mes'i,un &u'u, lemah.
@H?ase a'an memutus rantai ,olisa'ari!a men$a!i fra+men yan+ le-ih ,en!e'
0Na'amura/ et.al/ 199<1.
Ham-ar 5. 2'ema re,resentasi rea'si 'ataliti' @H?ase. 2etia, lin+'aran mewa'ili resi!u
+lu'osa/ lin+'aran ,utih men+in!i'asi'an +ula re!u'si. 0a1 rea'si 2i'lisasi/ 0-1 rea'si @ou,lin+/
0&1 rea'si !is,ro,orsionasi/ !an 0!1 rea'si hi!rolisis 0een et.al/ 20001
"e'anisme rea'si 'ataliti' en*im @H?ase miri, !en+an en*im L5amilase a'i-at
a!anya ,ersamaan stru'tur ,rimer !an tersier 'e!ua en*im terse-ut. @H?ase se&ara
s,esifi' men+'atalisis rea'si L51/<5trans+li'osilasi !an meru,a'an en*im utama !alam
analisis me'anisme rea'si en*im 'eluar+a L5amilase.
2i'lo!e'strin !i,ro!u'si se&ara en*imatis men++una'an en*im @H?ase !en+an
-ahan -a'u ,ati atau amilosa. @H?ase mem,ro!u'si si'lo!e'strin !ari ,ati melalui rea'si
trans+li'osilasi intramole'uler. Na'amura et.al 0199<1 -erhasil menyeli!i'i a'ti7itas
!is,ro,orsionasi en*im @H?ase men++una'an (5'eto-utili!ena5 E525'loro5<5nitrofenil5
malto,entaosi!a se-a+ai !onor. "elalui e's,erimennya/ ter-u'ti -ahwa rea'si
trans+li'osilasi oleh @H?ase -erlan+sun+ men++una'an me'anisme 4in+ 4on+ 6i 6i.
Dalam mem,ro!u'si si'lo!e'strin/ @H?ase a'an men+'atalisis 'eem,at rea'si
yan+ telah !ise-ut'an se-elumnya. Rea'si &ou,lin+ yaitu ,eme&ahan &in&in si'lo!e'strin
sehin++a ter-entu' maltooli+osa'ari!a. Rea'si !is,ro,orsionasi a!alah ,eme&ahan
maltooli+osa'ari!a yan+ men+hasila'an maltooli+osa'ari!a lain !an +ula ,ere!u'si yan+
!a,at men+ham-at ,em-entu'an si'lo!e'strin. Gntu' men+atasi hal terse-ut ,roses
hi!rolisis harus !a,at su-strat !en+an rantai +lu'osa yan+ sesuai untu' ,roses si'lisasi
!alam ,em-entu'an si'lo!e'strin. ?aha, rea'si ,ertama !alam ,roses ,ro!u'si
si'lo!e'strin a!alah hi!rolisis. .i!rolisis ,ati a!alah ,roses ,eru-ahan sus,ensi +ranula
,ati men$a!i larutan !e'strin yan+ larut !en+an 7is'ositas ren!ah. "ole'ul +ranula ,ati
-era!a !alam 'on!isi ,oli'ristalin -ersusun !en+an +aya inter !an intra mole'ul yan+
men+i'atnya sehin++a ,ati ti!a' larut !alam air !in+in !an larut !alam air ,anas. =arena
!alam 'on!isi ,anas a'ti7itas en*im menin+'at. Gntu' men+hasil'an !e'strin yan+
-anya' saat ,roses hi!rolisis !a,at !i+un'an 'om-inasi en*im ,en+hi!rolisis yaitu L5
amilase/ E5 amylase !an +lu'oamilase. 2ema'in -esar 'onsentrasi en*im yan+ !i+una'an
ma'a sema'in -esar ,ati yan+ terhi!rolisis 0 Noor/ 20111.
310 Peran Enzim CGTase (alam Teknoloi Panan
Rea'si trans+li'olisasi @H?ase $u+a !i&o-a untu' mentransfer senyawa sa'ari!a
'e senyawa fla7onoi! 0%ahyuntari/ 20051. #la7onoi! ,a!a umumnya ter!a,at ,a!a
,ermu'aan atau sel e,i!ermis !aun yan+ -erwarna hi$au yan+ !i!u+a mem,unyai
'emam,uan ,rote'si terha!a, ra!iasi sinar ultra 7iolet !an mene'an foto,ero'si!asi
!alam 'loro,las. 2etelah !itrans+li'olisasi/ fla7onoi! yan+ semula ti!a' larut !alam air
a'an larut !alam air sehin++a fla7onoi! terse-ut memun+'in'an untu' !imanfaat'an
se-a+ai -ahan fun+sional fisiolo+is untu' ,an+an !an o-at5o-atan. #la7onoi! yan+
mem,unyai a'ti7itas hi,otensif men$a!i mun+'in untu' !i'em-an+'an se-a+ai -ahan
,elin!un+ terha!a, ra!iasi0%ahyuntari/ 20051.
Ham-ar 8. 4eran )n*im @H?ase !alam -i!an+ te'nolo+i ,an+an
3101/ Peranan enzim CGTase dalam 6em7entukan $iklodekstrin
?a,io'a meru,a'an salah satu sum-er ,ati yan+ ,otensial !i+una'an se-a+ai su-strat
untu' ,ro!u'si si'lo!e'strin. =on!isi su-strat san+at mem,en+aruhi a'ti7itas en*im
!"ase !alam men+'atalisis rea'si ,em-entu'an si'lo!e'strin. ?a,io'a $u+a men+an!un+
'om,onen amilo,e'tin yan+ relatif tin++i ya'ni 78/285>(M . Amilo,e'tin meru,a'an
,olimer yan+ tersusun !ari unit +lu'osa !en+an stru'tur -er&a-an+. Rantai !asarnya a!alah
'onformasi antara +lu'osa !en+an i'atan L51/< !en+an titi' ,er&a-an+an ,a!a i'atan L51/8
!an rata5rata ,an$an+ setia, rantai &a-an+ se'itar 20525 unit +lu'osa 0La+a/ 20011.
"e'anisme @H?ase men+'atalisis ,em-entu'an &y&lo!e:trin !imulai !en+an
,en+i'atan !ela,an sam,ai se,uluh unit +lu'osa !ari mole'ul ,ati. Rea'si terse-ut !imulai
,a!a rantai +lu'osa !ari u$un+ non5,ere!u'si. 2isi a'tif !ari @H?ase ter!iri !ari !ela,an
sam,ai se,uluh 0atau le-ih1 subsite 0unit +lu'osa yan+ teri'at !en+an @H?ase1 yan+
-eri'atan !en+an mole'ul +lu'osa. 2e-a+ai &ontoh/ me'anisme ,em-entu'an L5
&y&lo!e:trin ter$a!i melalui &ara ,en+i'atan !ela,an unit +lu'osa oleh L5@H?ase/
selan$utnya ,emutusan i'atan L51/<5+li'osi!i' antara subsite 'e!ua !an 'eti+a. .asil antara
!ari rea'si terse-ut a!alah maltohe'sosa. "altohe'sosa terse-ut selan$utnya tersi'lisasi
melalui u$un+ non5,ere!u'si yan+ -eri'atan !en+an subsite 'eti+a/ sehin++a mem-entu'
i'atan L51/< +li'oso!i' -aru antara unit +lu'osa satu !en+an 'eenam !ari
si'lomaltohe'sosa 0Hoel/ 199<1.
4em-entu'an si'lo!e'strin ,a!a suhu 80
o
@ meru,a'an 'on!isi ter-ai' yan+
!i,eroleh !en+an lama rea'si 210 menit 0(.5 $am1 !en+an ,erolehan si'lo!e'strin 1<0/20
+NL 0nilai 'on7ersi 70/10M1. 4emoton+an rantai &a-an+ amilo,e'tin !ari ,ullulanase
mem-entu' rantai lurus/ menye-a-'an ,em-entu'an si'lo!e'strin -erlan+sun+ !en+an
&e,at. Lama rea'si terse-ut le-ih sin+'at !i-an!in+ ,ro!u'si si'lo!e'strin men++una'an
hi!rolisat ta,io'a !en+an ,roses minimalisasi ase,tor/ ya'ni 280 menit 0< $am 20 menit1
0La+a/ 20011. ?er!a,at 'e&en!erun+an ,enurunan si'lo!e'strin setelah $umlah ,erolehan
yan+ ma'simum/ !ise-a-'an 'arena si'lo!e'strin men+alami !e'om,osisi 'em-ali oleh
a'ti7itas @H?ase melalui rea'si &ou,lin+. Rea'si &ou,lin+ terse-ut !ise-a-'an oleh
a'umulasi +ula ,ere!u'si yan+ -ersifat se-a+ai ase,tor. A'umulasi ,ro!u' sam,in+ !alam
-entu' +ula se!erhana terse-ut/ !a,at men++an++u rea'si si'lisasi !an sta-ilitas ,ro!u'
si'lo!e'strin 0La+a/ 200>1.
*A* &)
PE%"T"P
01/ Kesim6ulan
6er!asar'an ,en$elasan ,a!a -a- se-elumnya/ ma'a !a,at !isim,ul'an -ahwa :
1. )n*im @H?ase meru,a'an en*im !en+an famili yan+ sama !en+an 5amilase yan+ -iasa
!ise-ut famili 1( +li'osil5hi!rolase
2. )n*im @H?ases !a,at !i,ro!u'si !ari -a'teri Bacillus sp, Micrococcus sp, B. macerans,
B. licheni#ormis, !an Bacillus circulans. )n*im @H?ases !a,at !i,eroleh !en+an &ara
fermentasi ,a!a me!ia 'ultur.
3. )n*im @H?ase mam,u men+'atalisis em,at $enis rea'si : si'lisasi/ coupling/
!is,ro,orsionasi/ !an hi!rolisis. "e'anisme rea'si 'ataliti'nya miri, !en+an en*im 5
amilase.
<. Dalam in!ustri !an te'nolo+i ,an+an/ en*im @H?ase -er,eran !alam mem,ro!u'si
si'lo!e'strin yan+ -erasal !ari ,ati terli'uifi'asi.
01- $aran
Graian tentan+ ,eran @H?ase !alam mem,ro!u'si si'lo!e'strin meru,a'an to,i'
,em-ahasan yan+ menari' $i'a !i+ali le-ih !alam. .al ini !a,at men$a!i re'omen!asi !alam
,em-ahasan ma'alah selan$utnya yan+ -ertema se$enis.
(A'TA# P"$TAKA
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Li6ase 'rom *acillus s61 !ournal *iotec2noloy and A66lied *ioc2emistry. ol. (8.
.lm 7512. 4un$a- Gni7ersity. @han!i+arh.
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@ham,ai+n.G2A.
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La+a/ A. 200>. Modifikasi Ta6ioka denan Proses Likuifikasi $ecara Enzimatis. 9.
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Lehnin+er/ A.L. 1995. (asar5dasar *iokimia 3. )rlan++a. 9a'arta.
Na'amura/ A./ .a+a/ =./ an! Yamane/ =./ 199</ ?he trans+ly&osylation rea&tion of &y&lo!e:trin
+lu&anotransferases o,erate! -y a ,in+ ,on+ me&hanism. FEBS Letters/ ol ((70199<1
,+. 88570.
Noor/ )rli*a/ an! Lies-etini .artoto. PP#<("K$& $&KL<(EK$T#&% (A#& PAT& GA#"T
ME%GG"%AKA% *E#*AGA& K<M*&%A$& E%=&M >Production of Cyclodextrin
from Arro:root $tarc2 7y "sin t2e Com7ination Enzymes?.P$urnal "eknologi Dan
Industri Pangan 22.2 020111: 1(7.
4oe!$ia!i/ A./ #.". ?. 2u,riyanti. 2008. (asar5(asar *iokimia. G354ress. 9a'arta.
2tryer/ L. 2000. *iokimia. ol 2. )!isi <. 4ener-it 6u'u =e!o'teran )H@. 9a'arta.
2un/ ?ao/ et al. PProduction of a no9el Cyclodextrin lycosyltransferase from *acillus s61
$K/31 ..-1P A#rican $ournal o# Microbiology Research 7.20 0201(1: 2(1152(15.
?on'o7a/ A./ 199>. *acterial Cyclodextrin Glucanotransferase. )n*yme an! "i&ro-ial
?e&hnolo+y 22: 87>58>8. )lse7ier 2&ien&e 3n&.
een/ 6. A./ Git!ehaa+/ 9.@./ an! 4ennin+a/ D./2000/ Rational Desi+n of @y&lo!e:trin
Hly&osyltransferase from Bacillus circulans 2train 251 to 3n&rease a5@y&lo!e:trin ,ro!u&tion/
J.Mol.Biol 298/ 1027510(>.
%ahyuntari/ 6u!iasih. 2005. $iklodekstrin Glikolisis Transferase dan Pemanfaatannya
dalam &ndustri1 9urnal ?e'nolo+i !an 3n!ustri 4an+an. 9a'arta