Wang Lin

(汪 琳）
 Before a tissue can be observe with a light
microscope, it must be made into a section. The
routine histological preparation for light microscope

examination is a paraffin section stained with

haematoxylin and eosin (abbreviate HE ).
This is a picture of muscle cells,
HE staining. The nucleus of the
cell is blue which stained
by haematoxylin(a basic dye ).
So the nucleus is said to
be basophilic.

The cytoplasm of the cell is

pink which stained by
eosin(an acidic dye ). So the
cytoplasm is termed acidophilic.
Section Number 17: Nervous cell
A cell includes three parts:
Cell membrane or plasma membrane
Cytoplasm
Nucleus
Specimen number 13.

Smooth muscle cells are fusiform,. Each cell has a single , ellipsoid
nucleus located in the center of the broadest part of the cell. HE
Specimen number 14
Anterior
horn

Gray
matter

Central
canal
posterior
horn

Cross section of spinal cord(HE stain,low mag.)

Nissl body(rER and ribosome)

Nucleus

Nucleolus

Nervous cell, HE Stain

 Section of liver showing Mitochondria

Mitochondria can be seen with the light microscope in specially

stained preparations. They are so called because they appear either
as granules or as rods. .
Section number 20 : Nervous cell showing cytoskeleton.

Cytoskeleton
microfilaments,
microtubules and
intermediate
filaments
They can been seen
under LM by using
Silver Stain.
 Section of nerve ganglion showing Golgi Complex. Silver stain.

In light microscopic preparations suitably treated with silver salts the

Golgi complex can be seen as a small structure of irregular shape,
usually present near the nucleus. The Golgi complex is the place where
the secretory products are packaged within membrane-bound vesicles.
No.13 Smooth muscle cells (HE stain)
No.14 Spinal cord (HE stain, showing RER and Ribosome in
the neuron)
No.17 Sympathetic ganglion (HE stain, showing the neuron)
No.20 Spinal cord (silver stain, showing cytoskeleton)
G （ Golgi complex) (silver stain, showing Golgi
complex in the neuron)
M （ Mitochondria) Liver( special stain, showing
Mitochondria in the hepatocyte)
Drawing:
1.Smooth muscle cell
2.A neuron in No.14 specimen
3.A neuron in No.17 specimen