Nicole Mae D.C.

Martinez
Regina Thea V. Agulan
Tc-99m Pertechnetate
Ultra-Tag Kit
In Vitro method
Tc-99m Pertechnetate
Ultra-Tag Kit
In Vitro method
Tc-99m as the pertechnetate ion is not firmly
bound to red blood cells and will diffuse into the
extravascular fluid compartment, with
accumulation in organs such as the stomach, gut
and thyroid gland.


Tc-99m
Pertechnetate +
Stannous Ions
Pertechnetate
ion (with Tc-
99m in the +7
oxidation state)
Non-reactive
Bind to
Hemoglobin
Tc-99m Pertechnetate
Ultra-Tag Kit
In Vitro method
1. A 10 milliliter reaction vial containing:

* Stannous Chloride. Dihydrate (SnCl22H2O) : 50
105 g
* Sodium Citrate Dihydrate 3.67 mg
* Dextrose, Anhydrous 5.50 mg

2. Syringe I (to be protected by light) contains:

* Sodium Hypochlorite 0.6 mg in Sterile Water
for Injection



3. Syringe II contains:

* Citric Acid, Monohydrate 8.7 mg
* Sodium Citrate, Dihydrate 32.5 mg
* Dextrose, Anhydrous 12.0 mg in Sterile Water
for Injection

Tc-99m Pertechnetate
Ultra-Tag Kit
In Vitro method


1 3 mL of
autologous
whole
blood
Citrate
complex
stannous
ion and the
dextrose
Incubation
period of 5
mins.


The hypochlorite solution of syringe I
is added.
The solution in syringe II is added.
A pertechnetate solution (0.53 ml,
3703700 MBq) is added.
The technetium Tc 99m-labeled
red blood cells are then reinjected
intravenously into the patient for
gamma scintigraphic imaging.
Red blood cell labeling efficiency of
95% is typically obtained using this
in vitro labeling procedure.
http://www.nuclearonline.org/PI/MallicknrodtUlt
ratagforR.
http://mariorad.com/books/GeneralradiologyDia
gnosticNuclear0Medicine-A.L.Baert,Christiaan
Schiepers
http://pharmacyce.unm.edu/nuclear_program/fr
eelessonfiles/Vol12Lesson1.pdf