Extraction Conditions Responses Stevioside Content (%) A Sugar Content (%) TSS (°brix) PH

Extraction Conditions
Responses
Stevioside Content (%)
S.No.
A:Time (min)
B:Power (Watts)
C:Ratio (Leaf:Methanol) (g:ml)
1.25
540
1:7.5
2.0
900
1:7.5
2.0
180
1:7.5
2.0
540
1:5
0.5
900
1:7.5
1.25
540
1:7.5
1.25
540
1:7.5
1.25
180
1:5
1.25
540
1:7.5
10
0.5
540
1:5
11
0.5
180
1:7.5
12
1.25
180
1:10
13
1.25
900
1:10
14
0.5
540
1:10
15
1.25
540
1:7.5
16
1.25
900
1:5
17
2.0
540
1:10
A420
R1
R2
Sugar Content (%)

R2
R3
R1
R2
R3
4.59
3.19
4.49
8.94
9.04
7.64
1.37
2.6
3.9
10.9
11
9.6
1.23
3.44
2.04
3.34
7.85
7.95
6.55
6.6 5.2 6.5
1.381 1.271 1.281
3.64
2.24
3.54
8.59
8.69
7.29
6.2 4.8 6.1
8.5
1.371 1.261 1.271
4.54
3.14
4.44
8.6
8.7
7.3
7.8
9.1
1.325 1.215 1.225
4.83
3.43
4.73
8.18
8.28
6.88
6.6 5.2 6.5
9.5
8.1
9.4
1.303 1.193 1.203
4.98
3.58
4.88
8.72
8.82
7.42
6.6 5.2 6.5
5.8
4.4
5.7
1.291 1.181 1.191
3.14
1.74
3.04
6.81
6.91
5.51
6.5 5.1 6.4
10
8.6
9.9
1.329 1.219 1.229
5.23
3.83
5.13
8.3
8.4
6.5 5.1 6.4
5.6
6.9
1.185 1.195 1.295
3.74
2.34
3.64
6.44
7.74
7.84
6.6 5.2 6.5
6.2
7.5
7.6
1.074 1.084 1.184
4.29
2.89
4.19
6.41
7.71
7.81
5.6 6.9
6.4
7.7
7.8
1.151 1.161 1.261
2.74
4.04
4.14
6.57
7.87
7.97
5.4 6.7 6.8
7.3
7.4
1.305 1.315 1.415
2.54
3.84
3.94
7.21
8.51
8.61
6.4
7.7
7.8
0.971 0.981 1.081
2.98
4.28
4.38
5.54
6.84
6.94
5.4 6.7 6.8
7.3
8.6
8.7
1.207 1.217 1.317
3.19
4.49
4.59
7.3
8.6
8.7
5.2 6.5 6.6
7.3
8.6
8.7
1.311 1.321 1.421
3.19
4.49
4.59
9.25 10.55 10.65 4.9 6.2 6.3
5.3
6.6
6.7
1.282 1.292 1.392
2.19
3.49
3.59
6.22
R2
R3
8.7
7.3
8.6
7.5
6.1
7.4
1.47
1.36
6.4
6.3
1.33
1.22
6.5
5.1
6.4
8.6
7.2
9.2
1.281 1.171 1.181
Extraction conditions
S.No.
A:Time (min)
B:Power (Watts)
pH
R1
R1
R3
TSS (Brix)
7.52
7.62
R1
R2
6.7 5.3 6.6

6
6.4
4.6 5.9
A420
Sugar Content (%)
TSS (Brix)
6.3
6.3 6.4
5.1 6.4 6.5
Responses
C:Ratio (Leaf:Water) (g:ml)
R3
pH
R1
1
0.50
540
1:25
0.50
180
1:15
1.25
180
1:5
0.50
540
1:5
1.25
540
1:15
2.00
540
1:25
1.25
540
1:15
1.25
540
1:15
1.25
540
1:15
10
2.00
180
1:15
11
1.25
900
1:5
12
1.25
180
1:25
13
1.25
900
1:25
14
0.50
900
1:15
15
2.00
540
1:5
16
1.25
540
1:15
17
2.00
900
1:15
R2
R3
R1
R2
A:Time (Min)
B:Temperature (Celsius)
R1
R2
R3
R1
R2
R3
R1
R2
7.4
1.073
1.183
1.083
2.78
4.18
4.08
7.3
7.4
5.5
6.8
6.9
7.4
5.3
1.075
1.185
1.085
2.11
3.51
3.41
5.8
7.1
7.2
5.6
6.9
5.3
5.6
1.15
1.26
1.16
2.24
3.64
3.54
7.6
8.9
6.3
6.4
5.6
7.3
1.347
1.457
1.357
3.08
4.48
4.38
5.7
7.1
5.2
6.5
6.6
7.3
9.8
1.218
1.328
1.228
3.99
5.39
5.29
7.2
8.5
8.6
5.3
6.6
6.7
9.8
6.6
1.084
1.194
1.094
2.74
4.14
4.04
7.5
8.8
8.9
5.3
6.6
6.7
6.6
8.4
1.214
1.324
1.224
3.8
5.2
5.1
7.9
9.2
9.3
5.2
6.5
6.6
8.4
1.216
1.326
1.226
3.42
4.82
4.72
7.4
8.7
8.8
5.1
6.4
6.5
9.4
1.249
1.359
1.259
5.09
5.19
3.79
8.8
8.9
7.5
5.4
6.7
6.8
9.4
1.215
1.325
1.225
3.02
3.12
1.72
9.7
9.8
8.4
5.5
6.8
6.9
1.358
1.468
1.368
4.52
4.62
3.22
10
10.1
8.7
4.4
5.7
5.8
7.4
1.072
1.182
1.082
3.84
3.94
2.54
7.7
7.8
6.4
7.2
5.8
7.1
7.4
9.6
1.356
1.466
1.366
4.42
4.52
3.12
9.9
10
8.6
6.1
4.7
9.6
8.4
1.359
1.469
1.369
4.1
2.7
9.3
9.4
5.9
4.5
5.8
8.4
6.6
1.316
1.426
1.326
3.36
3.46
2.06
9.7
9.8
8.4
4.6
5.9
6.6
9.7
1.248
1.358
1.258
4.86
4.96
3.56
9.1
9.2
7.8
6.7
5.3
6.6
9.7
6.6
1.405
1.515
1.415
3.36
3.46
2.06
11.5
11.6
10.2
6.1
4.7
6.6
S.No
R3
Responses
C:Ratio (Leaf:Methanol) (g:ml)
A420
Sugar Content (%)
TSS (Brix)
pH
40
45
1:5
25
45
1:7.5
10
60
1:7.5
40
45
1:10
25
45
1:7.5
25
45
1:7.5
40
60
1:7.5
25
45
1:7.5
40
30
1:7.5
10
25
60
1:5
11
25
45
1:7.5
7.2
1.52 1.42 1.41 2.3 3.7 3.6

4. 5.
5.8 7.1
6
6
6 8 8 8 8.08 9.38 9.48 6 6 9
10.
1
1.30 1.20 1.19 3.7 5.1 5.0

6. 4. 6.
8.7 10
4
4
4 9 9 9 7.23 8.53 8.63 3 9 2
9.2
1.06 0.96 0.95 3.3 4.7 4.6

6. 4. 6.
7.8 9.1
8
8
8 5 5 5 7.57 8.87 8.97 3 9 2
7.1
5.7
1.30 1.20 1.19 2.3 3.7 3.6

6. 5. 6.
7
4
4
4 3 3 3 7.44 8.74 8.84 5 1 4
9.3
1.18 1.08 1.07

6.
6.
7.9 9.2
2
2
2 4.8 3.4 4.7 7.66 8.96 9.06 4 5 3
10.
7
10. 1.18 1.29 1.19 5.4 4.0 5.3

6.
6.
9.3 6
5
5
5 9 9 9 7.9 9.2 9.3 4 5 3
8.1
6.7
1.39 1.49 1.38 4.2 2.8 4.1 10.3 11.6 11.7 5. 4. 5.

8
4
4
4 2 2 2
3
3
3 9 5 8
9.8
1.20 1.30 1.19 5.0 3.6 4.9

6. 4. 6.
8.4 9.7
1
1
1 5 5 5 7.33 8.63 8.73 3 9 2
5.4
1.32 1.42 1.31 3.5 2.1 3.4

6. 5. 6.
6.8 6.7
6
6
6 8 8 8 6.44 7.74 7.84 6 2 5
7.9
1.31 1.41 1.30

11.2 11.3 6. 4. 6.
9.3 9.2
5
5
5 4.8 3.4 4.7 9.96
6
6 2 8 1
7.9
1.18 1.28 1.17

9.3 9.2
1
1
1 4.8 3.4 4.7 7.6
6.
6.
9 8.9 4 5 3
1.20 1.30 1.19 3.2 1.8 3.1

6.
6.
6.4 6.3
2
2
2 9 9 9 7.52 7.62 6.22 4 5 3
12
25
30
1:5
13
25
60
1:10
6.7
14
10
45
1:5
6.1
1.09 1.19 1.08 4.0 2.6 3.9

6. 6.
7.4 7.5
2
2
2 5 5 5 8.74 8.84 7.44 3 4 5
15
10
30
1:7.5
7.2
8.5 8.6
16
25
30
1:10
6.9
0.94 1.05 0.95 2.9 4.3 4.2

6. 6. 5.
8.2 8.3
7
7
7 1 1 1 8.39 8.49 7.09 5 6 2
17
10
45
1:10
6.7
8 8.1
8 8.1
1.07 1.17 1.06 3.4 2.0 3.3

6. 6. 4.
2
2
2 3 3 3 8.96 9.06 7.66 2 3 9
0.92 1.03 0.93 3.0 4.4 4.3

6. 6. 5.
5
5
5 6 6 6 8.22 8.32 6.92 7 8 4
0.91 1.02 0.92 2.8 4.2 4.1

6. 6. 5.
5
5
5 2 2 2 7.29 7.39 5.99 6 7 3
S. No.
A:Time (Min)
B:Temperature
(C)
Responses
C:Ratio
(Leaf:Water)
(g:ml)
Stevioside Content
(%)
R1
R2
R3
A420
R1
R2
Sugar Content
(%)
R3 R1
R2
TSS (Brix)
pH
R3 R1
R2
R3
R R R
1 2 3
70
90
1:5
7.1
8.4
8.5
1.29
1.28 4.2
2.9 9.
1.154
4.38
4
4
8
8 2
9.3
7.9
6. 6. 5.
4 5 1
120
60
1:5
5.2
6.5
6.6
1.33
1.32 3.1
1.8 9.
1.191
3.26
1
1
6
6 7
9.8
8.4
6. 6.
5
3 4
20
90
1:15
8.3
8.4
1.37
1.36 4.2
2.9 7.
1.235
4.33
5
5
3
3 2
7.3
5.9
6. 6. 5.
6 7 3
120
90
1:15
7.3
7.4
1.43
1.42 3.8
2.5 7.
1.299
3.95
9
9
5
5 9
6.6
6. 6.
5
3 4
120
60
1:25
5.1
6.4
6.5
1.16
1.15 3.3
2.0 7.
1.029
3.41
9
9
1
1 8
7.9
6.5
6. 6. 5.
5 6 2
120
30
1:15
4.8
6.2
6.1
1.17
1.16 1.8
3.1 7.
1.032
3.26
2
2
6
6 3
7.4
6. 6. 5.
4 5 1
70
60
1:15
8.4
9.8
9.7
1.02
1.16 3.6
4.9 5.
1.159
5.01
9
9
1
1 8
7.2
7.1
5. 6. 6.
2 6 5
20
30
1:15
6.5
7.9
7.8
0.97
1.11 2.6
3.9 5.
1.109
4.09
9
9
9
9 6
6.9
6. 6. 6.
4 6 5
70
60
1:15
7.1
8.5
8.4 1.06 1.196 1.20 2.9 4.38 4.2 6.
5.6
5. 6. 6.
3 7 6
10
20
60
1:5
5.5
6.9
6.8
1.00
1.14
8.
1.130
3.5 2.2 3.6
0
0
2
9.6
9.5
6. 6. 5.
6 7 3
11
70
30
1:5
5.8
5.9
4.5
1.02
1.16 2.9
3.0 7.
1.154
1.62
4
4
2
2 6
8.9
6. 5. 6.
7 3 6
12
70
90
1:25
7.3
7.4
1.25
1.38 3.7
3.8 6.
1.395
2.44
5
5
4
4 4
7.7
7.8
6. 6. 5.
6 7 3
13
70
60
1:15
8.4
8.5
7.1
1.09
1.22 4.2
4.3 5.
1.239
2.98
9
9
8
8 4
6.7
6.8
6. 5. 6.
6 3 7
14
20
60
1:25
7.3
7.4
0.92
1.05 3.7
3.8
1.061
2.41
5
1
1
1
1
6.3
6.4
6. 6. 5.
8 9 5
15
70
60
1:15
9.1
9.2
7.8
1.02
1.16 4.6
4.7 6.
1.152
3.32
2
2
2
2 4
5.1
6.5
5. 6. 6.
2 6 5
16
70
30
1:25
6.2
7.6
7.5
0.92
1.06 3.8
3.9 6.
1.052
2.54
2
2
4
4 6
6.7
5.3
5. 6. 6.
5 9 8
17
70
60
1:15
7.6
8.9
1.10
1.24 4.5
4.6 6.
1.232
3.22
2
2
2
2 9
6.4
5.3
5. 6. 6.
3 7 6
Table 4.1: Effect of extraction conditions on product responses

Extraction Conditions Responses Stevioside Content (%) A Sugar Content (%) TSS (°brix) PH

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Extraction Conditions Responses Stevioside Content (%) A Sugar Content (%) TSS (°brix) PH

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Extraction Conditions

C:Ratio (Leaf:Methanol) (g:ml)

Sugar Content (%)

6.6 5.2 6.5

1.381 1.271 1.281

6.2 4.8 6.1

1.371 1.261 1.271

1.325 1.215 1.225

6.6 5.2 6.5

1.303 1.193 1.203

6.6 5.2 6.5

1.291 1.181 1.191

6.5 5.1 6.4

1.329 1.219 1.229

6.5 5.1 6.4

1.185 1.195 1.295

6.6 5.2 6.5

1.074 1.084 1.184

1.151 1.161 1.261

5.4 6.7 6.8

1.305 1.315 1.415

0.971 0.981 1.081

5.4 6.7 6.8

1.207 1.217 1.317

5.2 6.5 6.6

1.311 1.321 1.421

9.25 10.55 10.65 4.9 6.2 6.3

1.282 1.292 1.392

1.281 1.171 1.181

6.7 5.3 6.6

Stevioside Content (%)

Sugar Content (%)

5.1 6.4 6.5

Stevioside Content (%)

Sugar Content (%)

1.52 1.42 1.41 2.3 3.7 3.6

1.30 1.20 1.19 3.7 5.1 5.0

1.06 0.96 0.95 3.3 4.7 4.6

1.30 1.20 1.19 2.3 3.7 3.6

1.18 1.08 1.07

10. 1.18 1.29 1.19 5.4 4.0 5.3

1.39 1.49 1.38 4.2 2.8 4.1 10.3 11.6 11.7 5. 4. 5.

1.20 1.30 1.19 5.0 3.6 4.9

1.32 1.42 1.31 3.5 2.1 3.4

1.31 1.41 1.30

1.18 1.28 1.17

1.20 1.30 1.19 3.2 1.8 3.1

1.09 1.19 1.08 4.0 2.6 3.9

0.94 1.05 0.95 2.9 4.3 4.2

1.07 1.17 1.06 3.4 2.0 3.3

0.92 1.03 0.93 3.0 4.4 4.3

0.91 1.02 0.92 2.8 4.2 4.1

8.4 1.06 1.196 1.20 2.9 4.38 4.2 6.

Table 4.1: Effect of extraction conditions on product responses

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