Presented By: Ashley Palafox

Christina Watson James Yuanxin Li

Amy Choi Lucy Renderos
Tayo Oyesiku Montana Horwitz
Sam Cohen Jennifer Heimbach



By Ashley Palafox
Top: 40x, 1s, gain 2.0, B
Mid: 40x, 432.6ms, G
Bot: 40x, 218.5ms, R
Leica DM500 Brightfield 40x, 23.2ms
Elastic Fibers
By Sam Cohen
By Sam Cohen
Leica DM500 100x
L: Red, R: Green
By Jennifer Heimbach
A
B
C D
Zeiss AxioImager
A. Bright Field 20x
1 ms/ linear
B. Cy 3 20x 15 ms
Linear
C. GFP 20x 1 ms/
linear
D. Overlay: A, B, C
Leica DM500 10x fluorescence
By Ashley Palafox
Top L: 10x, 1sec, gain 2.6,
B

Top R: 10x, 252.9ms, gain
1.5, R

Bot L: 476.9ms, gain 1, G

Bot R: Overlay of all
fluor. channels
Lacunae +
Chondrocytes
Zone of
Proliferation
epiphysis
7
By Jennifer Heimbach (see next slide for metadata)
By Jennifer Heimbach
Zeiss AxioImager
Bright Field: 645ms/ linear/ 45% rheo Cy3: 1.3s/ linear
By Sam Cohen
Leica DFC400
BF-155.3ms Green-155.3ms Blue-2.2s UV-105.1ms
By Montana Horwitz
Leica DFC 400 40x
Exp = 105.1 ms, Gain = 2.5x, Sat = 1.10, Gamma = 1.01
By Tayo Oyesiku
Neutrophil
Leica DFC 400 40x Florescence
By Tayo Oyesiku
Green: exp =1.0s, sat = 3.0, gam = 0.65
UV: bright = 73%, sat = 1.75, gam =1.01
Blue: exp =1.0s, sat = 2.85, gam = 0.37
G
UV
B
By Christina Watson
Data
Exposure
179.7
Gain -1.0
Saturation
-1.55
Gamma -
1.37
By Christina Watson
Eosinophil
Echinocyte
Data
Exposure-
121.7ms
Gain- 1.0x
Situation- 1.75
Gamma -0.90

Neutrophil
Basophil
Zeiss Imager A1
BF-22.0ms Cy3-5.2ms Dapi-555.0ms GFP-513.0ms
By Montana Horwitz
By Lucy Renderos
Cartilage is usually found in
close association with bone in
the body. It is a type of
connective tissue which is
tough, semi-transparent,
elastic and flexible. The
matrix or ground substance of
cartilage consists mainly of
glyco-protein material,
chondroitin. The cartilage
cells (chondrocytes) lie
scattered in the matrix.
Cartilage is covered by a dense
fibrous membrane, the
perichondrium..
By Ashley Palafox
Top: 10x, 1s, gain 2.6, B
Mid: 10x, 500.7ms, G
Bot: 10x, 265.6ms, R
Leica DM500 Brightfield 10x, 11.2ms
Elastin Fibers
Lacuna +
Chondrocyte
Matrix
Perichondrium
By Lucy Renderos
Bone is a
strong,
flexible and
semi-rigid
supporting
tissue. It can
withstand
compression
forces, and
yet it can
bend. Like
cartilage, and
other types of
connective
tissue, bone
is made up of
Cells and
Extracellular
matrix:
By Amy Choi
Chrondrocytes
Osteoblasts
Periochondrium
Lacunae
Fibroblast
Territorial Matrix
by Amy Choi
Leica, 40x By Amy Choi
Leica 10x
By Beverly Howard
Mast
Cells
LeicaDM500 BF 100x, 23ms
By Amy Choi
Mast
Cell
Macrophage
Elastic
fibers
Collagen
fibers
Plasma
Cells
Leica DFC 400, 10x Brightfield
Brightness = 73%, sat = 2.85, gam = 0.37
By Tayo Oyesiku
Leica DFC 400 10x
Top (L, R): Green, Blue, Bottom: UV
By Tayo Oyesiku
G: exp = 3.4s, gain = 3.1x, sat = 2.85, gam= 0.37
B: exp = 1.0s, gain = 3.1x, sat = 2.85, gam= 0.57
UV: exp = 3.1s, gain = 3.1x, sat = 2.85
gam= 0.37
Leica DM500 40X
Top (L, R): UV A filter, Green N2.1 filter, Bottom (L, R): Blue i3 filter, Overlay
By James Yuanxin Li
Leica DM500 40X Brightfield
By James Yuanxin Li
1. Stratum
corneum
2. Stratum
lucidum
3. Stratum
granulosum
4. Stratum
spinosum
5. Stratum
basale
6. Dermal
papillae
Leica DM500 10X Brightfield
By James Yuanxin Li
1. Ciliated
epithelium
(pseudostratified
columnar)
2. Seromucous
glands
3. Smooth muscle
4. Hyline cartilage
5. Adventitia

Leica DM500 40X
Top (L, R): UV A filter, Green N2.1 filter, Bottom (L, R): Blue i3 filter, Overlay
By James Yuanxin Li
Echinocyte
Platelets
Erythrocytes
Erythrocytes, or red blood
cells, make hemoglobin,
which binds oxygen and
delivers it to the bodys
tissues.

Echinocytes are old red
blood cells which have
started to deteriorate, and
will eventually get trapped
and destroyed in the
spleen.

Platelets are made from
the cytoplasm of
megakaryocytes. They
help to seal wounds and
clot the blood.
By Ashley Palafox 100x, 115.9ms, BF, Leica DM500
Basophils are
involved in the
immune response to
parasites, and release
heparin and
histamine.

Neutrophils perform
phagocytosis, and
destroy bacteria and
damaged tissue.
By Ashley Palafox 100x, 115.9ms, BF, Leica DM500

Basophil
Neutrophil
Band cells are
immature
neutrophils.

Lymphocytes may
be either B cells,
which develop into
plasma cells and
produce antibodies,
or T cells, which
attack viruses and
cancer cells.
Lymphocyte
Band Cell
By Ashley Palafox 100x, 115.9ms, BF, Leica DM500

By James Yuanxin Li Leica DM500, Brightfield, 40x
Large coarse
granules containing
digestive enzymes.
Kills parasitic
worms. Modulates
immune response.

Diameter: 10-14
m.
2-4% of WBCs.
Development: ~14
days
Lifespan: ~5 days
By James Yuanxin Li Leica DM500, Brightfield, 40x
Monocytes:
Phagocytizes
bacteria. Develops
into macrophages in
the tissues.
Diameter: 14-24 m.
3-8% of WBCs.
Development: 2-3
days
Lifespan: months

Platelets:
Cytoplasmic
fragments of
megakaryocytes.
Instrumental in
blood clotting.
Diameter: 2-4 m.
Development: 4-5
days
Lifespan: 5-10 days

34
By Montana Horwitz
Lymphocytes- A lymphocyte is any of 3 types of white blood cell in a vertebrate's immune system. All 3 are
agranulocytes. They include natural killer cells (NK cells) (which function in cell-mediated, cytotoxic innate
immunity), T cells (for cell-mediated, cytotoxic adaptive immunity), and B cells (for humoral, antibody-driven adaptive
immunity). They are the main type of cell found in lymph, which prompted the name lymphocyte
Platelets- Platelets, also called "thrombocytes", are blood cells whose function (along with the coagulation factors) is
to stop bleeding.
[1]
Platelets have no nucleus: they are fragments of cytoplasm which are derived from the
megakaryocytes
[2]
of the bone marrow, and then enter the circulation. These unactivated platelets are biconvex discoid
structures
[3][4]
shaped like a lens, 23 m in greatest diameter.
[5]
Platelets are found only in mammals, an adaptation
that may have evolved to offset the risk of death from hemorrhage at childbirth a risk unique to mammals.
[6]

On a stained blood smear, platelets appear as dark purple spots, about 20% the diameter of red blood cells. The smear
is used to examine platelets for size, shape, qualitative number, and clumping. The ratio of platelets to red blood cells
in a healthy adult is 1:10 to 1:20.
Monocytes- Monocytes are a type of white blood cells (leukocytes). They are the largest of all leukocytes. They are
part of the innate immune system of vertebrates including all mammals (humans included), birds, reptiles, and fish.
They are amoeboid in shape, having clear cytoplasm. Monocytes have bean-shaped nuclei that are unilobar, which
makes them one of the types of mononuclear leukocytes (agranulocytes). Monocytes constitute 2% to 10% of all
leukocytes in the human body. They play multiple roles in immune function. Such roles include: (1) replenishing
resident macrophages under normal states, and (2) in response to inflammation signals, monocytes can move quickly
(approx. 812 hours) to sites of infection in the tissues and divide/differentiate into macrophages and dendritic cells to
elicit an immune response. Half of them are stored in the spleen
[1]
(except in people who have undergone
splenectomy). Monocytes are usually identified in stained smears by their large kidney shaped or notched nucleus.
These change into macrophages after entering into the tissue spaces.

35
Definitions via wikipedia
T
36
Happy Face Neutrophil neutrophil, type of white blood cell (leukocyte) that is characterized histologically by its ability to be
stained by neutral dyes and functionally by its role in mediating immune responses against infectious microorganisms.
Neutrophils, along with eosinophils and basophils, constitute a group of white blood cells known as granulocytes. The
granules of neutrophils typically stain pink or purple-blue following treatment with a dye. About 50 to 80 percent of all the
white bloods cells occurring in the human body are neutrophils.

By Montana
Horwitz