Blood Groups

Antigen agglutinogen
Antibody agglutinin
Reaction: agglutination

ABO
Blood Type
Ag Ab Anti - A Anti - B
A A Anti - B + -
B B Anti - A - +
AB AB - + +
O - Anti-A, anti-B - -
Rh
Blood Type
Anti - D
Rh (+) Agglutination
Rh (-) No agglutination
Material:
Lancet
70% alc
2 slides/member (3members)
Typing sera (anti-A, anti-B, anti-D)

** sharps container
yellow bag



Procedure:
1. Label slides A, B, D
2. Sterilize finger 70% alc
3. Puncture finger
4. Wipe 1
st
drop of blood
5. Place 1gtt of blood on
slide A
slide B
slide D
6. Press punctured finger on cotton
7. Add 1gtt of anti A on slide A
add 1gtt of anti B on slide B
add 1gtt anti D on slide D
8. Mix blood and typing sera using applicator stick (1 per slide)
9. Gently tilt slide back and forth for 1min
10. Observe reaction (clumping) under fluorescent microscope
11. Record result