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  • Plating Isolates On SNA at 22-25°C For 5-7 Days Agar Transfer To CMC Broth@ 22-25°C For 5-7 Days Spore Suspension Store at 4°C Until Use

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    tasneem06ft303
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    The document provides instructions for isolating and growing fungi from soil samples. The process involves plating soil samples on selective nutrient agar and incubating to isolate fungal colonies, transferring colonies to carboxymethyl cellulose broth to produce fungal spores, and storing the resulting spore suspensions at 4 degrees Celsius until use.

    Original Description:

    it is about fungal spore preparation.

    Original Title

    Spore Suspension

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    The document provides instructions for isolating and growing fungi from soil samples. The process involves plating soil samples on selective nutrient agar and incubating to isolate fungal colonies, transferring colonies to carboxymethyl cellulose broth to produce fungal spores, and storing the resulting spore suspensions at 4 degrees Celsius until use.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    8 views3 pages

    Plating Isolates On SNA at 22-25°C For 5-7 Days Agar Transfer To CMC Broth@ 22-25°C For 5-7 Days Spore Suspension Store at 4°C Until Use

    Uploaded by

    tasneem06ft303
    The document provides instructions for isolating and growing fungi from soil samples. The process involves plating soil samples on selective nutrient agar and incubating to isolate fungal colonies, transferring colonies to carboxymethyl cellulose broth to produce fungal spores, and storing the resulting spore suspensions at 4 degrees Celsius until use.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 3

    a)

    b)

    Plating isolates on SNA @


    22-25C for 5-7 days

    c)

    Agar transfer to CMC broth@ 22-25C for 5-7 days


    Spore suspension
    store @ 4C until use

    a)

    b)

    c)

    c)
    a)

    b)

    e)
    d)

    Autoclaved wheat, 35%


    m.c

    Autoclaved wheat, 35%


    m.c after 7 days

    ATR crystal in contact with


    mycelia

    ATR crystal indentation o


    mycelia

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