Professional Documents
Culture Documents
week 5
Lab Report
(sections with a * MAY be done with
group)
Introduction
Introduce the topic you are about to present (1 paragraph 1 page)
Materials*
List
Methods*
What did you do? Step by step. Be specific and please number steps.
Results*
No interpretation here! Just tell me what you saw.
Discussion
Now you interpret what you found. Why do you think you obtained the results you obtained? Were you surprised to see
those results? Why or why not? Did anything unanticipated happen that may have altered your results? (Did someone
sneeze on your plate while it was open?)
Conclusion
Close the report by stating anything you would have done differently if you had to do it again or by suggesting another
experiment based on the results of this one.
objectives
Keywords
Learning outcomes
Taxonomy
Prokaryote
Virus
Bacteriophage
Capsid
Capsomere
Lytic cycle
Lysogenic cycle
Prion
Algae
Fungi
Protozoa
Create a microbe
helminthes
Taxonomy
eukaryotes
prokaryotes
Morphology
Genetic features
Fossil records
Metabolic reactions
Genetic relatedness
Other properties
Difficult to classify
Fewer than 1% of known bacteria are completely classified from
domain to species
We know genus, species, strain/subspecies but its difficult to know
their entire evolutionary relationship in order to classify them
completely
viruses
debate continues as to whether theyre alive or not as they have no
means of replicating their own genetic information
infectious
intracellular hosts required
Viruses
range in size from
240nm (the size of
the smallest
bacteria) to less
than 30nm (the size
of a ribosome)
host cells
viruses need a host cell
the host range of a virus is the spectrum of hosts that a virus can
infect
most viruses have a viral specificity limited to a certain species and
even specific tissues within a specific host
ex: HIV attacks human CD4 T cells
HPV infects human skin cells
viral classifications
viruses are classified based on:
viral species
a viral species is defined as a group of viruses that share the same
genome and the same relationships with organisms
ex: FIV is a very similar genetically to HIV but they are not the same viral
species due to their relationship with different organisms (cats vs. humans)
viral species names do not follow binomial nomenclature and are in English
DNA
most are double stranded and families
separated based on shape of the DNA
(linear or circular), capsid shape and
presence of an envelope
Herpesviridae (enveloped, linear dsDNA
viruses. Large family of animal viruses
that always includes a latancy period,
such as chickenpox into shingles. HSV1,
HSV2, and Epstein-Barr are also in this
family.
Poxviridae (enveloped, linear dsDNA and
include small pox)
Papovaviridae (naked, polyhedral viruses
that replicate in the nuclei of host cells,
such as HPV)
emerging viruses
viruses have been in the human population as long as there have
been humans
emerging viruses are those that were present in very low levels but due to
human movement and population density and are increasing or
were able to cross the species barrier and infect humans
ex: influenza
when more than one strain of influenza is present in a host, they can swap part of their
genomes which can result in an increase in their ability to infect a wider range of hosts,
increase their pathogenicity, or increase the severity of the disease
penetration
synthesis
maturation
release
virus: infectious, once its inside a cell and is using the host cells machinery for its own purpose
virion: inert form, holds genetic information
viruses
attack animal hosts, but may also attack bacteria!
bacteriophages
highly specific, allowing them to be used in phage therapy to treat diseases without
killing normal microbiota in the patient
turn to p. 288, look at figure 10.12
You can do phage counts by doing serial dilutions on a bacterial lawn and counting the
plaque-forming units.
videos
replication of a virulent bacteriophage
replication of a temperate bacteriophage
replication of an enveloped dsDNA animal virus
replication of a + sense RNA virus: poliovirus
replication of a + sense RNA virus: HIV
Prions
have you heard of Creutzfeldt-Jacob disease? kuro? bovine
spongiform encephalopathy (mad cow disease)?
neurological diseases that always end in death and are not caused by a living
organism or a virus but by a proteinacious infectious particle called a prion
prions
prions have the following characteristics:
Protist Characteristics
kingdom Protista
[mostly] unicellular, eukaroyotic organisms
while debate is ongoing, were going to focus on the two
classifications:
plant-like (algae)
animal-like (protozoa)
algae
photoautotrophic
mostly aquatic
diatoms: important producers in aquatic environments
dinoflagellates: second most important producers in marine environments
there are species within both groups that are capable of producing neurotoxins.
When these species bloom, heterotrophic organisms such as shellfish consume a large
number of these algae and the neurotoxins build up in the shellfish tissue
when humans eat the shellfish, they get a large dose of the toxin, resulting in shellfish
poisoning
dolphins may also die
protozoa
heterotrophic
unicellular
large and diverse group but we will focus on parasitic protozoa
mastigophorans have flagella and include Trypanosoma (African Sleeping
Sickness), Leishmania (responsible skin lesions), Giardia (responsible for
diarrhea) and Trichomonas (responsible for vaginal infections).
Apicomplexans are immobile and include malaria (very complicated life cycle)
Amoebozoa move by pseudopods,
several species that cause diarrhea
Fungi
diverse group of heterotrophic organisms that can be unicellular
(yeast)or multicellular (mold).
body of the fungus is called a thallus which consists of mycelium, a
loosely organized structure of threadlike hyphae.
The cell wall contains chitin, a polysaccharide.
can reproduce both sexually and asexually, often switching between
them generation
fungi
highly important
decomposers, produce antibiotics and can be parasitic
parasitic fungi have three requirements to be successful
proximity to host
ability to penetrate the host
and ability to digest and absorb nutrients from host cell
parasitic fungi often kill the cell so they can decompose it
human fungal diseases (mycoses) can be classified as superficial, subcutaneous, systemic
or opportunistic.
fungi
superficial
affect only keratinized tissue in skin, hair and nails (athletes foot)
subcutaneous
affect skin layer beneath keratinized tissue to the lymph vessels (Candida
yeast infection)
systemic
involve internal organs (lung infection Histoplasmosis)
opportunistic
caused by fungi that only causes disease when host is already weakened
(fungal pneumonia in an AIDS patient)
helminths
parasitic worms
flatworms are primitive worms with simple digestive tracts
(roundworms, nematodes, flukes and tapeworms)
roundworms and nematodes have a primitive body cavity separating
their digestive system
most are hermaphroditic
includes earthworms
we will look at flukes, tapeworms, adult roundworms and roundworm
larvae