Professional Documents
Culture Documents
Week 4
presented by Amanda Arceo
sterilization
disinfectant
antiseptic
modes of action
physical methods
chemical methods
antibiotics
antibiotic resistance
Keywords
learning outcomes
Welcome back!...
Difference?
sterilization
Difference?
disinfectants
disinfection
killing enough
microbes that
infection is not
possible with the
number present
antiseptics
Microbial Control
Disinfectant=Dead; Antiseptic=Alive
Remember?
Scenarios
Things to consider
Use-Dilution Test
denaturing proteins
viruses
heat
refrigeration
dessication
irradiation
filtration
heat
dry
molecules are
oxidized
takes longer and can
only be used on items
that will not melt
under the
temperatures
open flame may be
used (ex: loops in lab)
moist
autoclave
increase atmospheric
pressure to increase boiling
point
pasteurization
alternative to
pasteurization (UHT)
Question
cold sterilization?
yeast
endospores
dessication
ultraviolet light
ionizing radiation
X-rays and gamma rays (short wavelengths)
microwave radiation
longer wavelengths
requires rotation
radiation
example?
antibiotic
selective toxicity
selective toxicity
Spectrum activity: range of bacteria species that the antibiotic can treat
Broad Spectrum Antibiotics? *
Narrow Spectrum Antibiotics?
susceptibility to superinfection
antibiotic function
WHY?
antimetabolites
Antibiotic Resistance
they resist!
transduction
conjugation
5 mechanisms of
resistance
alteration of targets
alteration of membrane
permeability
development of enzymes
alteration of an enzyme
alteration of a metabolic
pathway
ideal antibiotics