PENICK & FORD LIMITED INTER-OFFICE CORRESPONDENCE D.5623 pave: February 6, 1970 copies To. 10: Dr. Phed: H.A.Katz row: oR. D. Harvey Re: Glucose Isomerase Pilot Plant study In regard to your memo of January 26, 1970, we have a column which is 14 inches x 10 feet. We are making plans to have at our disposal substrate from any point in processing i.e., filtration, carbon treatment, or deionization. There are some factors which we feel will be important in your investigations in the laboratory and in a pilot plant trial. 1) Use of Filtered syrup It is suggested the use of filtered syrup be avoided. After filtration, syrup contains a very fine precipitate which tends to flocculate on aging. The content of this material is inconsistent and dependent on several factors: syrup quality, flow rate, type of filter media, and condition of filter cake. Floccy material such as described above will tend to absorb on the cellulose column and, undoubtedly, affect the system. 2) Use of Carbon Refined syrup As syrup is processed, the ash content increases. Carbon treatment significantly increases ash content. However, this would be one point that could be examined as a substrate supply. Do you have information indicating which metals have a poisoning or inhibitory effect on the enzyme and what levels of such metals are tolerable? It may be advisable for us to begin running assays, for such metals, on our product stream.D.5623 Page 2 February 6, 1970 3) Use of Deionized syrup It appears that deionized syrup would be the ideal substrate. The major objection here is the requirement of an additional deionization. 4) Concentration of Substrate Will there be any desire to concentrate the substrate? At this D.E. and temperature, viscosity is not a significant problem. With higher concentrations it may be possible to convert more substrate (lbs. of Dry Substance) for a given bed volume in a specified time period. 5) The 14" diameter column will be wrapped with a hot water hose and insulated to maintain the temperature at 140°F. Please supply us with the flow rate and the percent solids of the proposed feed syrup. What refining steps are expected after isomerization. The Bendix Polarimeter has been sent to you for recalibration and modification for higher fructose levels. RDH/oe ztpO EeBes