Sequence of the sense strand DNA encoding your SELEX library:

CCGAAGCTTAATACGACTCACTATA

G
GGAGCTCAGAATAAACGCTCAA[N1
50]TTCGACATGAGGCCCGGATCCGGC
Theprimersusedforpolymerasechainreaction(PCR)and
reversetranscription(RT)havethefollowingsequences:
5Primer
CCGAAGCTTAATACGACTCACTATA

G
GGAGCTCAGAATAAACGCTCAA
3Primer
GCCGGATCCGGGCCTCATGTCGAA
Notes
1.Theinitiallibrarycontainsa50baselongrandomized
region,designatedas[N150].Atthelevelofthelibrary
pool,thereisanequalprobabilityoffindinganyofthe
fourbasesateachpositioninthisregion.However,any
singlemoleculederivedfromthispoolhasauniquebaseat
eachpositionderivedfromthisregion.
2.TheT7promoterisunderlined.Thefirstbasetobe
incorporatedintoyourRNAtranscriptionproductistheG
showninboldface.Therefore,anRNAtranscriptderivedfrom
thislibrarywillhavethesequence:
GGGAGCUCAGAAUAAACGCUCAA[N150]UUCGACAUGAGGCCCGGAUCCGGC
TheDNAsequencedatafor30differentclonesobtainedat
theendofyourSELEXprocesstoidentifyhemebinding
aptamersisgivenbelow.Thesequencecorrespondstothe
N150regiononly.
>Clone_63
TGGTTTCAGCGACAGGAGGGGTGTAGGTGGATTGCTGTCCTTTGCGTGT
>Clone_65
CTTATGCAGTTTTACAGGGGTGATATGACTGCACCAGTAGGGGGATGTTC
>Clone_81
TGCTAGTGTTGTATGCACGTGGAGGAGGAGGCGTACACTTGCTTTGTGGT
>Clone_83
TGCAGAAGTTGCGTGTGGAGGAGGTGGCATGACTGCTGTTAGGGTAGTTG
>Clone_85
GGGATCTGGTTGAAACTGGAGGCCTATAGAAGTTGGTTGTGTGTTTTGAG
>Clone_86

GTCCTGTGATGGTATTTTTCGTTCCGCTTACTTCGACATGAGGCCCGGATCCATCTGAAT
>Clone_87
GATTGACCGTATGGAGGATGCAAAGGGAGGGAGGTCACTTGAGTTAGTTA
>Clone_88
GCAGGATGTGGAGGAGGCATCTGCTGCAATCGGGACTTGTGTCGAGTATC
>Clone_812
GCATTGTCTGCGTGTGGAGGCAGGAGGCAAGATAAGAGGTGATGCGGTTG
>Clone_813
CATGTTGGCGATACGTCTAAACGGTGGGTTGTGGAGGATTGATTTATACG
>Clone_819
AGTAGTGTCAGCGTGTGGTGGAGGTTGGCGACATATGTAGGGTGCGATTG
>Clone_82
GGGATCTGGTTGAAACTGGAGGCCTATAGAAGTTGGTTGTGTGTTTTGAG
>Clone_820
CGAAGGCACTTCATGGGGTGGAGGAGGCATGCGAGGTGTCCGGCGAGTGG
>Clone_810
TGCTAGTGTTGTATGCACGTGGAGGAGGAGGCGTACACTTGCTTTGTGGT
>Clone_818
GATTGACCGTATGGAGGATGCATAGGGAGGGAGGTCACTTGAATTAGTTA
>Clone_826
CACACGTGACTGTGGAGGCAGCGGAGGCGAGTTATGTGATGTTAAGAGGT
>Clone_828
TATGTTAAGAGGCCACTGATGCGCGTAGGTCTCTGGGGATTGAGGAAGGT
>Clone_830
AGGTTGCGCTAGGTGAGGAAGGAGGTGTAGGTACGGCCTATTGAGTGGGA
>Clone_831
CGTAGTCCATGAGTGTCTTTAGCTAACGGTTGGTAGTGAACCATATCCTG
>Clone_832
GCCAATGAGAGCTGTAGGAGGGCGGGACGTGCTTAGTGCGTGACACCGGA
>Clone_833
TTGTCCTGACTTGCTTGAACGTTAGCGTGATGCGTTATGCCCTGGATGGG
>Clone_816
GCATTGTCTGCGTGTGGAGGCAGGAGGCAAGATAAGAGGTGATGCGGTTG
>Clone_811
TGCTAGTGTTGTATGCACGTGGAGGAGGAGGCGTACACTTGCTTTGTGGT
>Clone_834
CACCAATGACGGGGGTTAAGACGGAGGGAGATGCATCGGTGTGAAGCTGA
>Clone_84
GGGATCTGGTTGAAACTGGAGGCCTATAGAAGTTGGTTGTGTGTTTTGAG
>Clone_835
TGCGCAATACACGGTGAGGAGGTGGAGAGATGTAGGTGCTTAGCAGTTGA
>Clone_817
GATTGACCGTATGGAGGATGCAAAGGGAGGGAGGTCACTTGAGTTAGTTA
>Clone_815
TGCTAGTGTTGTATGCACGTGGAGGAGGAGGCGTACACTTGCTTTGTGGT

>Clone_837
CGTGAACGCATGTGGTGGAGGAGGCGATTGCACGTGGGACCGAGCATTTG
>Clone_840
TGGACCGCAGCACGGCGCTCGTGGTAAGGCCGTATGCCCATCGAATGAAG