West China Journal of Pharmaceutical Sciences 2005-04

Determining the content of Thiamphenicol capsule

by HPLC
LI Cheng-ping~1,XU Hui-ying~1,CHEN Xue-song~1,SHAN Wei-guang~(2*) (1. College of
Biology and Environmental Engineering, Zhejiang Shuren University,Hangzhou
310015,China;2.College of Pharmaceutical Sciences,Zhejiang University of Technology,
Hangzhou 310032,China)
OBJECTIVE To determine the content of Thiamphenicol capsule.METHODS An HPLC method was
performed on an Agilent Eclipse XDB-C_8 column(4.6 mm250 mm,5 m)with the mobile phase consisted of
acetonitrile-water (2575). The flow rate was 1.0 mlmin~(-1) and detection wavelength was at 226 nm . The
injection volume was 20 l and external standard method was used.RESULTS The linear range of this method
was 10-400 g ml~(-1)(r = 0.9999) with the limit of detection of 2.0 ng . The average recovery of Thiamphenicol
was 99.6% to 101.1%,with RSD of 0.85% to 3.48%(n=5). CONCLUSION The method is
simple,sensitive,accurate and can be used for the determination of Thiamphenicol capsule.