A-kinase anchoring

proteins coordinate
inflammatory responses to
cigarette smoke in airway
smooth muscle
W.J. Poppingal, I.H. Heijink, L.J. Holtzer, P. Skroblin, E. Klussmann,
A.J. Halayko4, W. Timens, H. Maarsingh, and M. Schmidt

Published: January 30,2015 | doi: 10.1152/ajplung.00301.2014

Introduction - COPD

Chronic Obstructive Pulmonary Disease (COPD)

What causes COPD?
Smokers/Allergies/Pollution/Smog

Symptoms

Symptoms are typically

experienced at late
stages of the disease

Chronic cough
Mucus expulsion with cough

Frequent respiratory infections (acute flares)

Shortness of breath- exercise/fatigue
Loss of weight
Not enough O2 in blood

Category of Two diseases

1. Emphysema alveolar membrane breaks down
2. Chronic Bronchitis to much mucus, inflammation of lungs

Background
2-agonists inhibitors: bronchodilator (dilate airway of the lung)
Stimulate cAMP

Figure 2: Expression of AKAP5/12 mRNA is

altered in COPD pt and by CSE

Figure 6: AKAPs coordinate CSE-induced IL-8

release from hTERT ASM

Conclusion
Both CSE exposure and patients with COPD exhibit a decrease in
APKA5 and APKA12 expression
AKAP-PKA complex is important for decreasing IL-8 release
Overall:
AKAP appears to regulate and coordinate inflammatory response to
cigarette smoke in airway smooth muscle cells

Class Discussion Questions

1. Because cytokines do not work in isolation and can have systemic
effects, in what ways can we introduce and localize
pharmaceutical agents to specific areas or tissues (e.g. lung
tissue)
2. What issues can researchers run into regarding AKAP-PKA
interactions and inflammation when the results are examining
the relationship of COPD/CSE and AKAP5 and AKAP12 expression?

Sources
Source #1:
Poppinga WJ, Heijink IH, Holtzer LJ, Skroblin P, Klussmann E, Halayko AJ,
Timens W, Maarsingh H, Schmidt M;
A-kinase anchoring proteins coordinate inflammatory responses to cigarette
smoke in airway smooth muscle
,Am J Physiol Lung Cell Mol Physiol. 2015 Jan 30:ajplung.00301.2014. doi:
10.1152/ajplung.00301.2014. [Epub ahead of print]
http://www.webmd.com/lung/copd/tc/chronic-obstructive-pulmonarydisease-copd-overview
http://www.doping-prevention.sp.tum.de/substances-and-methods/beta2-agonists/beta-2-agonists.html