Genetic Engineering Project:

ENGINEERING DNA
Task:Inyourteams,youaretousetheconceptofgeneticengineeringtocreatecure/prevention
technique/minimizesymptomsforadiseaseandthenconvinceareviewboardfromtheSmallBusiness
Administrationthattheirproductanditsdeliverymechanismareworthyoffunding.
FinalProduct:Yourfinalproductwillincludealogbook,applicator(deliverymechanism),anda
presentation.

Youwillbuildanddesignanaestheticallypleasingapplicatorandapplicatorspackaging
displayingthecompanynameandlogo,usingrecyclablematerialsofanykindyoudonotneed
topurchaseanythingforyourproduce.
Youwillsubmitalogbookthathascopiesofdailygroupevaluations,labreports,copiesof
DNAextractionlab,andcopiesofthetransformationlabformoreinfo,seechecklist.
YouwillpresenttotheSBAreviewboardonthelastdayoftheproject.

StandardsCovered:
BS.5.3:ExplainthestructuralpropertiesofDNAandtheroleofDNAinheredityandprotein
synthesis.
BS.5.5:Explainchromosomalmutations,theirpossiblecauses,andtheireffectsongenetic
variation.
BS.2.1:Explainhowscientificadvancementsandemergingtechnologyhaveinfluencedsociety
BS.2.2:Comparerisksandbenefitsofpotentialsolutionstotechnologicalissues.
Otherinformation:
Inyourpresentation,youwillneedaddressthefollowing:
1. Youwillhavetounderstandyourproduct/medicineintermsofhowthediseaseworks.Indoing
so,youwillneedtoknowwhatorganand/orsystemittargetsandhowyourproductwillhelp
thatparticularsystem.Forexample,ifyourtargetdiseaseisdiabetes,youwillneedtoknow
whatorganandorgansystemdiabetestargetsandthemechanisminwhichitdoesaffectthe
system(diabetesaffectsorganssuchaskidneys,eyes,nerves,etc.)Yourproductismadeup,but
thesystemhastoberealintermsofthedisease.
2. Youwillthenneedtoproposeamechanisminwhichhowtheproductaffectsthesystemandto
whichorganandsystemitwilltarget.Todothis,youwillneedtobeclearofwhatyourproduct
isdoing.Willitcurethedisease?Willitpreventthediseasefromhappening?Willitminimize
thesymptoms?Forexample,producinginsulinandinjectingitintothehumanbodywillhelp
minimizethesymptomsofdiabetes,notcurethepatientofdiabetes.
3. Afteryouwillneedtodeviseamethodofadministrationthatmakessensetothedisease.For
example,ifyouwanttoinjectinsulinintothebloodstream,thelogicalmethodofapplication
wouldbethroughaneedle.Anotherexampleisifyourproducttargetsthelungs,youwillneedto
createanapplicatorandproductthatneedstobeinhaled.Ifyourproducttargetsrashesoritems
ontheskin,thentheappropriatemethodofadministrationwouldbeatopicalcream.

4. Afterdoingalllabsandfiguringoutappropriatemethodsofadministration,youwillneedto
discussandfigureouthowthetechniquesinthelaboratoryareusedtocreateamechanismin
whichhowyourproductisgoingtogofromthesourcetotheproduct.Todothis,youwillneed
toreferbacktoyourgeneticengineeringnotes(oryoucanresearchthis)andfigureoutasource
ofwhereyourproductcamefrom.Remember,thisprojectstemsfromtheconceptofgenetic
engineering,soyouwillneedtoextractthegenefromsomewhere,insertitintoabacterium,and
thencreateproduct.Soforexample,ifyouweretodoinsulin,theinsulingenewastakenfrom
animalstheninsertedintoE.coliinwhichthebacteriumproducestheinsulingenethathumans
cannowuseintheirbodies.
5. Afterresearchingandlearninghowyourproductworksandhowitwillbeused,youwillthen
needtofindawaytosellyourproducttotheboard.Youwillbegivenaseparateguidelinepaper
inordertoperformasolidpresentation.Thekeytothispartispractice,practice,practice!
Timeline:
Date
Tuesday,January27
Thursday,January29
Monday,February2
Tuesday,February3
Thursday,February5

Monday,February9

Tuesday,February10
Thursday,February12
Tuesday,February17
Thursday,February19

Activity
Reviewguidelines,turninnamesandduties,
companynameandlogo,possiblediseasesthat
youwanttotarget,gooverintroductionquestions.
DNAextractionlabwearshoes!!
DNATransformationlabwearshoes!!
DNATransformationlabpartIIwearshoes!!
Whatmakesagoodpresentation,brainstormand
researchproductcharacteristics,begindesigning
medicinalapplicator
Brainstormvariousapplicatorideasandbest
methodsforthismedicinetobeinsertedintothe
bodyandthendesignanapplicatorthatbest
appliesthisparticularmedicinetothebody,
designpackaging
BringinALLmaterialstobuildanapplicatorand
packagingincludingthecompanylogo.
Planpresentationcompanypresentationtothe
SmallBusinessReviewBoard
Practicepresentations!
Presentations!!!!!!!!!!

EngineeringDNA
Checklist
Usethefollowingchecklistasyouprogressthroughtheprojecttoensureyouhavemettheminimum
projectcriteria.YoumustalsomeetalloftheexpectationspresentedintheLogBookRubric,Applicator
Rubric,PresentationRubric,andPowerpointPresentation.

LogBook

DNAExtractionGenesinabottlelab

pGLOGeneticTransformationLab

ApplicatorPrototype

CompanyPresentation

Powerpointpresentation
EngineeringDNA
LogBookTableofContentsandRubric

Pleaseusethisrubricasaguidelineforcompletionofthelogbook.Thelogbookwillbecollectedatthe
endofthisproject.
____________CoverDesignwithCompanyLogoandNames/PositionsTyped
____________TableofContentsTyped
____________GeneticEngineering,anOverview(nomorethan1page)Typed
____________Writeupcontaining:(nomorethan2pages,doublespaced)Typed
a. Introductiontoyourdisease
b. Productdetailswhatisit,howdoesitwork(mechanism)intermsofyour
diseaseandhowtheproductaffectsthesystem,whereitwilltargetandmethod
of administration, and how the techniques used in lab describe how your
medicinegoesfromthesourcetoaproduct.
____________DNAExtractionLabTyped
____________pGLOGeneticTransformationLabTyped
____________DailyGroupEvaluationsHandwritten
____________CopyofyourpresentationTyped
____________GroupChecklist
____________Rubrics

*Everythingthatyouwriteoutinyourlogbook,needstobetypedexceptforyourevaluations*
EngineeringDNA
ApplicatorRubric
FivePointAssessment:1=Poor/Missing2=Fair3=Good4=VeryGood5=Excellent

12345 Applicatorisneatandshowsthoughtandeffortwentintodesignandbuild
12345 Applicatorhascompanynameandlogodisplayed
12345Applicator&Medicineislogicalandrealisticinitsdeliverymechanism
12345 Applicator&Medicineisappropriateinsize
12345 Packagingdisplaysdrugfacts:activeingredients,purpose,uses,warnings,
directions,otherinformation.
EngineeringDNA
PresentationRubric
FivePointAssessment:1=Poor/Missing2=Fair3=Good4=VeryGood5=Excellent
12345

Eachelementinthepresentationfollowedtheappropriatestructuringofthepresentation.
Introductionofgroupmembersandcompanyname,whatisyourdisease,whydowe
haveaneedtoresearchthisproblem,introductionoftheproduct,easeofproductusage,
summary,close,questions.Refertohandoutformoreinformation.

12345

Theinformationpresentedisdetailedenoughtobeeasilyunderstandbyyouraudience

12345

Showunderstandingandcanelaborateinresponsetoquestionsaboutscienceconcepts

12345

Studentspresentedwithpoiseanddressedappropriately.

12345

Time,energy,effortenthusiasm,andgroupcommitmenttotheprojectisevident

12345

Studentspresentedinawaythattheyknewtheinformationanddidnothavetoread
directlyofftheslides

12345

Presentationissmoothandshowsevidenceofpreparation

12345

Thepresentationisvisuallyappealing.

12345

Allgroupmembersparticipateinthepresentation,maintaineyecontactwithaudience,
anduseaclearvoicewithcorrectpronunciationofterms.
EngineeringDNA
PowerPointRubric

Usethisrubricasaguidelineforthecompletionofasuccessfulproject.Thechoiceofelectronic
applicationisdeterminedinyourteams.YoumayusePowerPoint,Prezi,WindowsMediaMaker,
Video,aBlog,orWebsiteitisuptoyou.Bemindfulifyouuseanyaudio/video/anystatistical
informationthatbelongstoothers,youmusthaveanaccurateReferencepage.
FivePointAssessment:1=Poor/Missing2=Fair3=Good4=VeryGood5=Excellent
12345

IntroductiontoCompanyandGroup

12345

ExplainDNAextraction,whatitisandhowitrelatestotheproduct

12345

ExplainpGLOcelltransformation,whatitisandhowitrelatestotheproduct

12345

Introducemedicineandexplainhowitworks

12345

Visualandaudioaspectsofthepresentationareeasyfortheaudicetoseeandunderstand

12345

Thepresentationcontainsbulletedorsummarizedinformationthatthestudentscan
elaborateon

12345

Thepresentationhasbeenproofreadandpreviewedtoensurenoerrorsarepresent.

12345

Thepresentationincludesanabundanceofappropriatepictures,diagrams,graphs,etc.

12345

ReferencePage(ifyouusedinformation,pictures,graphsetc.fromanysourceyou
MUSTcitethem)

Whatmakesagoodpresentation?
IntroductionofCompanyandNames
Whatisyourcompanyname?
Whoarethepeopleinyourteamandwhatdotheydo?

Backgroundinformation
Whatisyourdisease?
Whydoweneedtohaveaneedtoresearchthisproblem?

Statetheproblem
Whyisitsobad?
Whosaffected?
Whatstheprevalenceofthedisease?
Morbidityrate?
Isthetrendincreasing/decreasing?
IncidenceRate?
Symptoms?

Introducesolution
Whatcanmyproductdoforyou?
Howwillmyproductimproveyourlife?
Generalknowledgeofthebiologyoftheproduct?
Whatdoesyourproductdo?Cure?Reducesymptoms?Prevent?

Discusssolution
Typeofapplicator?
Isitfastorslowacting?
Howoftenwouldyouneedtouseit?
Comparethepricetoareallifeexpense.Isitworthitforthelessening/curingofthedisease?
Isthecostworththebenefit?

Summarizepresentation
Restatediseaseandproduct
Statebenefits
Driveyourproducthome
Onlytalkaboutpositivethingsaboutyourproduct

ClosePresentation
Thanktheaudienceforlistening.

HandlingQuestions&objections