052615-1

Cell line: SW480

loading conc: 125 ug

2
24h, C

052615-2
Cell line: SW620
loading conc: 125 ug

12

11
24h, C

10

Sample: 100uM drug treatment

Tube code: R1

5
24h, verat

8
24h, verat

7
MW

6
MW

9
48h, C

4
48h, C

10

11
12 (duplicate of 11)
48h, verat

2
48h, verat

060515-1
Cell line: SW480
loading conc: 150 ug

2
24h, C

060515-2
Cell line: SW620
loading conc: 150 ug

12

11
24h, C

10

Sample: 100uM drug treatment in 10% DME; tx time= 10' and 30'
Tube code: 10' or 30' label

5
24h, verat

8
24h, verat

7
MW

6
MW

9
48h, C

4
48h, C

10

11
48h, verat

12

2
48h, verat

060915-1
Cell line: SW480
loading conc: 150 ug

2
24h, C

060915-2
Cell line: SW620
loading conc: 150 ug

12

11
24h, C

10

Sample: Conservative readjustment based on mTOR expression of R1 samples (gels=0526

Tube code: R1

5
24h, verat

8
24h, verat

on of R1 samples (gels=052615)

7
MW

6
MW

9
48h, C

4
48h, C

10

11
12 (duplicate of 11)
48h, verat

2
48h, verat

061815-1
Cell line: SW480
loading conc: 150 ug

2
24h, C

061815-2
Cell line: SW620
loading conc: 150 ug

12

11
24h, C

10

Sample: 100uM drug treatment

Tube code: R2

5
24h, verat

8
48h, C

8
24h, verat

5
48h, C

10

11
48h, verat

12

2
48h, verat

062415-1
Cell line: SW480
loading conc: 140 ug

062415-2
Cell line: SW620
loading conc: 140 ug

12

Sample: 100 uM treatment

Tube code: R3

2
24h, C

11
24h, C

10

4
24h, verat

9
24h, verat

5
24h, verat

8
24h, verat

8
48h, C

5
48h, C

10
48h, verat

3
48h, verat

11
48h, verat

12

2
48h, verat

070815-1
Cell line: SW480

2
24h, C

loading conc: adjusted based

on mTOR expression on gel ()

070815-2
Cell line: SW620
loading conc: adjusted based
on mTOR expression on gel ()

12

11
24h, C

10
24h, verat

Sample: Readjusted conc. Based on mTOR expression from R1 sample set (R1 gels = a)
Tube code: R1

24h, verat

9
24h, verat

1 sample set (R1 gels = a)

MW

7
MW

9
48h, C

5
48h, C

10

11
48h, verat

12
blank

2
48h, verat

071015-1
Cell line: SW480
loading conc: 150 ug

071015-2
Cell line: SW620
loading conc: 150 ug

12

Sample: Re-reun of gels 061815

Tube code: R2

3
MW

11

10
MW

24h, C

24h, C

5
24h, verat

8
48h, C

8
24h, verat

5
48h, C

10

11
48h, verat

12

2
48h, verat

071315-2
Cell line: SW620
loading conc: 150 ug

12

11
24h, C

10
MW

Sample: Re-run R3 samples (gel 062415-2 did not properly transfer)

Tube code: R3

transfer)

8
24h, verat

5
48h, C

2
48h, verat

071515-1
Cell line: SW480
loading conc: 150 ug

071515-2
Cell line: SW620
loading conc: 150 ug

12

Sample: 10uM drug treatment

Tube code: A

24h, C

2
24h, C

11

10

4
24h, verat

9
24h, verat

5
24h, verat

h, verat

6
MW

7
MW

10
48h, verat

5
48h, C

48h, C

48h, verat

11

12
blank

2
48h, verat

h, verat

071615-1
Cell line: SW480
loading conc: 150 ug

Sample: Re-reun of gels 071515-1

Tube code: A

2
24h, C

5
24h, verat

6
MW

8
48h, C

9
48h, verat

10
48h, verat

11

12
blank

071615-2
Cell line: SW620
loading conc: 150 ug

Sample: Re-reun of gels 071015-2

Tube code: R2

12

11
24h, C

10

8
24h, verat

6
MW

4
48h, C

2
48h, verat

B= 48h only samples tx w/100uM but in 60mm plates