MacConkey Agar:

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Eosin methylene blue:

- Contains bile salts

- Inhibit G(+) bacteria
Lactose fermenters turn the colonies
pink.
Colonies of the non-lactose fermenters are
colourless.
- Inhibit G(+) bacteria
Bacteria that ferment lactose, especially
the coliform bacterium Escherichia coli,
appear as colonies with green metallic
sheen or blueblack to brown color.
Bacteria that do not ferment lactose appear
as colorless or transparent light purple
colonies

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Hektoen Enteric Agar:

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Thiosulfate Citrate Bile Salt Sucrose

agar plate (TCBS)

Medium designed to isolate and

differentiate members of the species
Salmonella and Shigella from other
Enterobacteriaceae.
- Bile salts and the dyes bromthymol blue
and acid fuchsin inihibit the growth of most
G(+) organisms.
- Lactose, sucrose, and salicin provide
fermentable carbohydrates to encourage
the growth and differentiation of enterics.
- Sodium thiosulfate provides a source of
sulfur. Ferric ammonium citrate also allows
the visualiztion of hydrogen sulfide
production by reacting with hydrogen
sulfide gas to form a black precipitate.
Salmonella typhi : Colorless colonies,
black centers.
- For the selective isolation of Vibrio
cholerae and Vibrio parahaemolyticus from
a variety of clinical specimens and in
epidemiological investigations.
Vibrio cholerae : Growth (yellow
colonies)

Vibrio parahaemolyticus : Growth (green

colonies)
Staphylococcus aureus : No growth

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Chocolate Agar:

Is prepared by adding sheep blood to an

enriched agar base when the temperature
of the medium is high enough to lyse the
red cell without inactivating the NAD (V
factor) and hematin (X factor) in the
blood lysate (about 80C)

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Thayer Martin VCN:

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Blood Agar:

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- with 5% chocolate sheep blood plus

- Vancomycin, which is able to kill most
Gram-positive organisms
- Colistin, which is added to kill most
Gram-negative organisms except Neisseria
- Nystatin, which can kill most fungi.

Pseudomonas aeruginosa (extra)

greenish-metallic appearing colonies
(production of the pigments pyoverdin and
pyocyanin)