Practical 1: Eubacteria

Experiment 1: Bacteria (Heterotrophic Bacteria): Are Bacteria Present In Lab?

Objective
1. To investigate the bacteria colonies.
2. To investigate the color and the texture of the bacteria growth.
Introduction

Materials
4 nutrient agar cultured plates, sterile cotton swab, marker, 2 bottles labeled A and B (A contains
tap water and B contains 70% ethyl alcohol), transparent adhesive tape, paper towels.
Procedure
1. Four petri dishes containing nutrient agar was labeled as Control for dish 1, Dry Swab for
dish 2, Treatment A for dish 3 and Treatment B for dish 4.
2. A sterile cotton swab was touched over the surface of the table and was strike on the agar
of Dish 2.
3. The lid on Dish 2 was securely taped to the bottom half of the dish.
4. One paper towel with tap water and second paper towel with 70% ethyl alcohol was
soaked.
5. The table was wiped down with one half of the surface with liquid A and other half with
liquid B.
6. After the table was dried, using Dish 3 and Dish 4, the procedure describe in step 2 was
repeated.
7. The entire cultured agar was placed in the incubator for two days to see the bacteria
colonies, color and the texture of the bacteria growth.

Result

FIGURE 1
Bacteria Colony on Difference Condition

d
Agar
labeled
Agar
labeled
swab. c
plate

plate
control.
plate
dry
Agar
labeled

Treatment A (tap water). d Agar plate labeled Treatment B (70% ethyl alcohol)

Discussion
From the experiment, there are four type of petri dish was labelled with Control, Dry
Swab, Treatment A and Treatment B which is to determine the colonies of bacteria, the colour
and the texture of the bacteria growth.
The control petri dish was not exposing to environmental and seal with parafilm.
However, the control petri dish was contaminated by a fungus. This is because; the environment
of the laboratory was already contaminated.

On top of that, in the dry swab petri dish, there are two colonies of bacteria was obtained.
For the first colony of the bacteria, the colour is yellow, while for the shape, it has a round shape
and the number of bacteria presence in the petri dish is only single bacteria. For the second
colony of the bacteria, it has white colour, round shape and the number of bacteria presence only
2.
For the treatment A, tap water was wiped on the surface of the table, and then sterile
cotton swab was run on the table randomly then was strike on nutrient agar. From the
experiment, the bacteria were growth in the petri dish. There are two colonies of bacteria which
bacteria that having a yellow colour and a white colour. For the shape both of them have a round
shape. Contamination also occurs in this dish.
For the treatment B, the surface of table was wiped with 70 % ethyl alcohol and then
sterile cotton swab was run on the table randomly then strike on nutrient agar. There are two
colonies of bacteria are growth in the plate. One of these bacteria is yellow in colour, the shape a
round and the number of colonies presence is three colonies. The other bacteria are white colour,
the shape of bacteria is round and the number of colonies presence is two colonies. In theory,
bacteria cannot growth in high concentration of 70 % ethyl alcohol. However, result that
obtained from experiment is against the theory, this is because agar on treatment B dish was
contaminated.
In conclusion, all of the agars were contaminated. So, there are several techniques to
prevent contamination to occur in the agar plate. For example, by using aseptic technique, all
material must be sterilize, the table must be spray with high concentration of alcohol, the petri
dishes and test tubes covered as much as possible, by using metal inoculating loops, the entire
piece of metal of the inoculation instrument was heat in the flame, it should be red hot, the
inoculating loops was let it to cool before picking the inoculum (broth or agar). This is because
microorganisms in the lab atmosphere may come to rest on the material and table between
classes and overnight. The sterilize method are used to kill the microorganisms at the material
and can reduce the percentage of contamination.

Conclusion

In conclusion, bacteria can growth in the dry swab and treatment A condition. While,
bacteria cannot grow in the high concentration of 70% ethyl alcohol. On top of that, the
morphology of bacteria identified have colour, round shape and smooth surface for both colonies
of bacteria.

Reference
1. Campbell, N. A. and Reece, J. B. (2008). Biology. 9th Edition
2. Solomon, E.P., Berg, L.R., & Martin, D.W. (2008). Biology. 9th Edition. Thomson.

UNIVERSITI TEKNOLOGI
MARA

PHY 320
Diploma in Science

Part 6 ASD6Cn
LAB REPORT

Prepared by:

NO.

NAME

MATRIC NUMBER

RAJA MUHAMMAD ALIFF ARIFFIN B.

RAJA SAHROM

2011629066

KASYFUL AZIM BIN FADIL

2011552003

AHMAD NAIM FIRDAUS B MOHD

YUSOFF

2011108177

MUHAMMAD ASYRAF B ABDUL

RAHMAN

2011933161