PLANT TISSUE CULTURE

oleh:
Elina Lestariyanti S. Pd

JOURNAL
REFERENCES

Plant Tissue Culture in Biotechnology:

Recent advances in Transformation
Through Somatic Embryogenesis
Particle Bombardment
Agrobacterium -Mediated
Transformation

Somatic Embryogenesis

PARTICLE
BOMBARDMENT

Methods for DNA delivery into plant cell,

were used for specific transformation
purposes and different types of cell and
genotypes. (1)

- Electroporation
- PEG (Polyethylen
Glycol)treatment
- Microinjection
- Sonication
- Silicon carbide
treatment

Helios Gen Gun

dan alat Gen Gun Standar

Agrobacterium -Mediated
Transformation

Method for transferring genes into plants

through the inoculation of leaf discs with
Agrobacterium tumeficiens followed by in
vitro culture and regeneration of whole
plants. (1)

crown gall tumor

WHY
Agrobacteri
um?

Agrobacterium tumefaciens is a natural

vector system for transgenes delivery
into a wide range of plants species. (1)

SOMATIC
EMBRYOGENESIS
Somatic embryogenesis is a process whereby somatic
cells differentiate into somatic embryos. (2)
In somatic embryogenesis the embryos regenerate from
somatic cells, tissue or organs.

Soybean
Transformation
Fig. 1. Scheme for isolation
of
immature
cotyledons
from a soybean pod (A),
induction
of
somatic
embryos on MS40 medium
(B) and embryogenic callus
on MS20 and FN Lite media
(C, D). Bar: 1 mm
Fig. 2. A Transient GFP
expression in soybean
embryogenic callus (1 day
after bombardment with
NPTII/GFP construct). B, C,
D GFP expression in
single somatic embryo,
callus and mature somatic
embryos obtained from
embryogenic callus after 9
days of culture on SHAM

Cotton Transformation

Fig. 3. Different
stages
of cotton hypocotyls
transformation

Fig. 4. Agrobacterium-mediated
transformation
of embryogenic callus:

(1)Sidorov, V. A. 2013. Plant Tissue Culture in

Biotechnology: Recent Advances In Transformation
Through Somatic Embryogenesis. Biotechnologia
Acta. 6(3).
(2)Arnold, S.V. 2008. Somatic Embryogenesis. In Plant
Propagation by Tissue Culture 3rd Edition. E. F.
George, et al. (eds). Netherland: Springer. pp 335.
(3)Purnamaningsih, R. 2002. Regenerasi Tanaman
melalui Embriogenesis Somatik dan Beberapa Gen
yang Mengendalikannya. Buletin Agrobio. 5(2): 55.