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THE EFFECT OF 10% GREEN TEA (Camellia sinensis) CATECHINS

ADDITION IN PERIODONTAL DRESSING ON


HUMAN PRIMARY FIBROBLAST
PROLIFERATION

(In vitro study)


Siti Ramadania W.*, Kwartarini Murdiastuti**, Hendrawati**
*Undergraduate program, Faculty of Dentistry, Universitas Gadjah Mada
**Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada
ABSTRACT
Background: Periodontal dressing was developed with some additives that aimed to
increase its value from a mechanical wound barrier to wound healing agent.
Fibroblast proliferation as one of wound healing indicator need to be assessed further.
One of the natural compounds with wound healing potential was green tea catechins
but recent studies about catechins effects regarding fibroblast proliferation had variety
of results, whether enhanced or suppressed fibroblast proliferation. Objective: This
study aimed to attain the effect of 10% green tea catechins addition in periodontal
dressing on human primary fibroblast proliferation. Methods: This study used 3
types of periodontal dressing: Baers periodontal dressing (PD), Baers periodontal
dressing with 10% green tea catechins addition (PDC), and Coe-PakTM (CP). Each
periodontal dressing was immersed in natural saliva for one day then added to human
primary fibroblast culture in the microplate and incubated for 24 and 72 hours. After
the incubation period, proliferation test conducted with MTT assay that resulted
optical density (OD) value. Data then analyzed by MANOVA, LSD, and Independent
T Test. Results: Statistical analysis of OD value after 24 hours incubation showed that
there were no significant differences (p>0,05) among CP, PD, and PDC whereas data
of 72 hours incubation showed significant differences (p<0,05). OD value of PDC
was lower than CP and PD while there was no significant difference (p>0,05) between
CP and PD. Conclusion: 10% green tea catechins addition in periodontal dressing
inhibited human primary fibroblast proliferation.
Keywords: Green tea catechins, periodontal dressing, proliferation, human primary
fibroblast