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Cell Biology
223.24: Actin Filaments and Cell Movement

General Introduction

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Actin Filaments (7nm)

ATP is hydrolyzed to ADP when monomers assemble

and can not exchange with ATP un:l the monomer
dissociates

Actin Filaments (7nm)

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Peripheral Membrane Proteins

spectrin

Actin Filaments (7nm)

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Actin Filaments (7nm)

Actin Filaments (7nm)

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Actin Filaments (7nm)

Actin Filaments (7nm)

filopodium

lamellipodium

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Actin Filaments (7nm)

Leading edge

Actin Filaments (7nm)

myosin motor proteins slide actin filament

contraction

Transmembrane adhesion
receptors (integrins) anchor
cell to the extracellular
matrix while associating
with actin intracellularly

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Actin Filaments (7nm)

Actin Filaments (7nm)

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Actin Filaments (7nm)

Actin Filaments and Muscle Contraction

Three basic muscle types

Smooth

Cardiac

Skeletal, striated

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Actin Filaments and Muscle Contraction

Actin Filaments and Muscle Contraction

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Actin Associates with Myosin to Form Contractile Structures

Myosin I has a tail and globular ATPase head.

Myosin I is found in all cells and it is involved in
vesicular movement and cell shape changes
Myosin II form dimers that
have a tail and globular ATPase heads.
Myosin II is found in muscle cells and
is important in muscle contraction

Actin Filaments and Muscle Contraction

Myosin II form thick filaments.

Myosin II is arranged as a dimer
with two globular heads and a coiledcoil tail.

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Actin Filaments and Muscle Contraction

Fig. 17-36 and 17-39

Actin Filaments and Muscle Contraction

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Actin Filaments and Muscle Contraction

Myosin head lacking bound
ATP attaches to actin filament.

ATP binds myosin head and myosin

releases actin.

ATP hydrolysis occurs but ADP and Pi

remain bound. The head is displaced by 5 nm
along the actin filament.

Actin Filaments and Muscle Contraction

Myosin releases Pi
and powerstroke
occurs. ADP is released.
Myosin again is bound to
actin.

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Actin Filaments and Muscle Contraction

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