..

UV-VIS.


:
mol=trans+vibr+Rot+
:
mol= ,
trans= ,
vibr= ,
Rot= ,
= ( ,
).

 ,

UV UV-VIS

(l x mol-1 x cm-1)
H2O : *, n * <190nm

 n* , n* , * , dd*
n*

: 270-290 nm
*
:

dd*
[Cu(H2O)6}++

n*
180nm :
190nm:
220nm:

 UV-VIS
UV-VIS


.

IR, NMR, MS
.

H 2

HOMO:Highest Occupied Molecular Orbital

LUMO: Lowest Occupied Molecular Orbital

 - * transition.


4-methyl-3-penten-2-one

236 nm , - * transition.
314 nm , n - * transition

UV-Vis

 -
1,3

1,3,5-

200-220 nm

Ethylene(CH2=CH2)
1.3-butadiene
Vitamin A
-carotene
Benzene
Naphthalene
Anthracene
Naphthacene

(peak)
180nm
217nm
328nm
450nm

255nm
286nm
375nm
477nm




, Red #3:

,
Blue #1:

UV-Vis
:
:

I .
.
10min
O


(.. pH )

O UV-Vis


UV-VIS,
1. , 2.,
3., 4., 5.

 UV-Vis
,

(
)

1. ,
2.

,
3. (
),
4.

0% 100%
5. ,
6.

3%

0.2 .


( )

W
.

D2, 2

180nm 350nm



(1200/mm)

 (slit)

: , ,


,
,


..


.

UV-VIS.

:
~ 2000 3000 Euro
(2004)
~ 15000 Euro (2004)

UV-VIS


Lambert -Beer
A= b c
.

:
1.

= =b c

=
=
=

=
.. ()

2. ..

 LambertBeer
:1.

2. .
3.
.
4.
5.
.

 -o

HPLC


UV, VIS

(
).
15%< <65%.

 UV-VIS
UV, VIS,
.
.
.
UV SiO2.

 UV-Vis

 UV-VIS

 .


.
cut-off
330m
( ..
...

).