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  • Analytical Biochemistry Modifik SDS PAGE Mali Molek

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    This document presents a new method of tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) for separating proteins in the range of 1 to 100 kDa. The method utilizes a discontinuous buffer system with tricine in the anode buffer and sodium dodecyl sulfate (SDS) in both the sample and gel buffers to improve resolution of proteins below 20 kDa compared to conventional SDS-PAGE. Tricine-SDS-PAGE allows for better separation and analysis of a wide range of protein sizes from 1 to 100 kDa on a single gel.

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    SDS PAGE Mali Molek

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    This document presents a new method of tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) for separating proteins in the range of 1 to 100 kDa. The method utilizes a discontinuous buffer system with tricine in the anode buffer and sodium dodecyl sulfate (SDS) in both the sample and gel buffers to improve resolution of proteins below 20 kDa compared to conventional SDS-PAGE. Tricine-SDS-PAGE allows for better separation and analysis of a wide range of protein sizes from 1 to 100 kDa on a single gel.

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    12 views1 page

    Analytical Biochemistry Modifik SDS PAGE Mali Molek

    Uploaded by

    skljole
    This document presents a new method of tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) for separating proteins in the range of 1 to 100 kDa. The method utilizes a discontinuous buffer system with tricine in the anode buffer and sodium dodecyl sulfate (SDS) in both the sample and gel buffers to improve resolution of proteins below 20 kDa compared to conventional SDS-PAGE. Tricine-SDS-PAGE allows for better separation and analysis of a wide range of protein sizes from 1 to 100 kDa on a single gel.

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    Analytical Biochemistry

    Volume 166, Issue 2, 1 November 1987, Pages 368379

    Tricine-sodium dodecyl sulfatepolyacrylamide gel electrophoresis for the


    separation of proteins in the range from 1 to
    100 kDa

    Hermann Schgger,

    Gebhard von Jagow

    Institut fr Physikalische Biochemie der Universitt Mnchen, Goethestrasse 33, 8000


    Mnchen 2, Federal Republic of Germany

    http://dx.doi.org/10.1016/0003-2697(87)90587-2, How to Cite or Link Using DOI

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