Biology Investigatory Project

on the topic

STUDY OF EFFECTS OF ANTIBIOTICS ON

MICRO ORGANISMS

INDEX

1.Certificate
2.Acknowledgement
3. Introduction
4. Experiment
5. Observation
6. Results &
Conclusion
7. Bibliography
CERTIFICATE OF AUTHENTICITY
This is certify that Dalveer Singh, a
student of class XII B Roll no. 8 has
successfully completed the research

project on the topic Study of Effect of

Antibiotics on Micro -organisms
under
the guidance of Mr. AD Prasad subject
teacher in year 2015-16. The references
taken in making this project have been
declared at the end of this report.

Sign

Sign

Sign

(subject teacher)

(examiner)

(principal)

Acknowledgement
I wish to express my deep gratitude and sincere
thanks to principal. Mr Joshua Hastings for his
encouragement and for all the facilities that he
provides for this project work. I sincerely
appreciate this magnanimity by taking me into
his fold for which I shall remain indeptedto him. I
extend my heartily thanks to Mr. AD Prasad.
(Biology teacher), who guided me to the
successful completion of this project. I take this
opportunity to express my deep sense of
gratitude for her invaluable guidance, constant

encouragement, which has sustained my efforts

at all stages of this project work. I cant forget to
offer my sincere thanks to my classmates who
helped me to carry out this project work
successfully and for their valuable advice and
support, which I received from them time to
time. I would also like to thank Mr Jai raj (lab
asst) for his support and guidance.
Dalveer Singh

Introduction
Antibiotics are the organic secretion
produced by micro organisms, which in low
concentrations are antagonistic to the
growth of other micro organisms (mostly
pathogens). Antibiotics have proved very
useful in combating several bacterial
diseases in man an animal. Antibiotics are
commonly obtained from actinomycetes and
some eubacteria. Some of the important
antibiotics are streptomycin, Aureomycin,
teramycin, Chloromycetin, erythromycin,
neomycin etc.

Soil is a natural medium that harbors

several types of micro organism
.These micro- organisms can be frown
on culture media. The effect of
different types of antibiotics can be
studied on the growth of micro organism growing in culture medium.
This is an important subject, therefore
the study of effect of antibiotics on
micro - organism has been taken for
the present project.

Objective of Project

The aim of this project is to

study the effect of antibiotics
on micro organisms.

Experiment
To study the effect of antibiotics on microorganisms

Requirements
Potato ,agar, dextrose , distilled water , four
different types of antibiotics (such as
penicillin , streptomycin , aureomycin ,
teramycin ) , syringe , oven sterilized
petridish , flasks , beakers , pipettes , garden
soil , glass marker pen , etc

Procedure
A. Preparation of culture Medium
1. Potato Dextrose Agar (PDA) Medium. Take
200 g of peeled potato chips. Boil them with
500 ml of water in a beaker for 15 minutes.
Squeeze the potato pulp thus obtained
through a muslin cloth and keep it in a flask.
Take 20 g of agar in a beaker and warm it
with 500 ml of water .Mix both the solution
of potato and agar and add 20 g dextrose to
it. Thus one litre of PDA medium is prepared.
Autoclave the medium at 15 pounds
pressure for 15 minutes.
B. Effects of antibiotics on soil micro0
organisms
i) Take 2 g of soil and dissolve it in 10 ml of

water in a beaker. Let the soil particle settle

down.
ii) Take 5 oven sterilized petridishes and pour
1 ml of soil suspension in each of the plates.
Now pour 1ml of the four antibiotics
separately in four petridishes with the help
of syringe, and mark them with marker pen.
Leave the fifth petridish without antibiotic to
serve as control.
iii) Pour PDA in each of the petridishes and
mix the suspension by rotating the
petridishes. Leave the petridishes
undisturbed at a warm place for an

Observation
Colonies of micro- organisms appear on the
culture medium, count the colonies in each
petridishes and present your observation in
the following table:

S.No. Antibiotics

No. of
colonies in
a petridish

1.

NIL

Pencillin

2.

Streptomycin

2-3

3.

Terramycin

NIL

4.

Chloromycetin

1-2

5.

Control

30-40

The effect of different antibiotics on the micro

organisms can be assessed by counting the
number and size of the colonies growing in the
petridishes.

Conclusion
Pencillin and Terramycin was most effective
antibiotic against microorganism in soil.

Bibliography
1. Comprehensive practical biology
2. Wikipedia