Professional Documents
Culture Documents
View
Theoretical plate
Chromatographic principle
Sample
mixture
Mobile phase
Equilibrium
establishes at each
point (ideally)
Stationary phase
Retardation of rate
of movement of
molecules
Different distribution
coefficients and different
net rates of migration
Greater
The
Where:
N = Number of theoretical plates
Ve = elution volume or retention time (mL, sec, or
cm)
h = peak height
w1/2 = width of the peak at half peak height (mL,
sec, or cm)