ustcwhm@ustc.edu.

cn

(Chinese phoenix/phoenix)

Eden

Paradise

WOMAN



1.

2.
3.

1995 (Joseph P. Vacanti)

(Charles A.Vacanti)Robert
Langer

Dr. Robert Langer.

Germeshausen Professor of Chemical and Biomedical Engineering at MIT
Dr. Joseph P. Vacanti.
John Homans Professor of Surgery at Harvard Medical School

,,,

Are there differences between cell engineering and

tissue engineering?

The UIC BioE Department website provides the following

description, cell and tissue engineering are very
complementary to one another as research in these fields often
blends together. For example,
cellular engineers use engineered scaffolds to study how cells
adhere to surfaces and the impact the characteristics of the
surface can have on cellular process such as cell proliferation
and migration.
Tissue engineers then use this knowledge to determine how best
to coat the surface of their scaffolds to stimulate cells to perform
as they desire.
It is then accurate to state that cell & tissue engineering is a
better description of the newly emerged and exciting
interdisciplinary field of biological and physical sciences.

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The Rotary Cell Culture SystemRCCS

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Hing KA, 2004

Hing KA, 2004

[Ca5(PO4)3OH
HA]

Hing KA, 2004

Science 18 November 2005:

Vol. 310. no. 5751, pp. 1135 - 1138
Molly M. Stevens and Julian H. George
Exploring and Engineering the Cell Surface Interface

Hierarchical organization of bone over

different length scales. Bone has a strong
calcified outer compact layer (A), which
comprises many cylindrical Haversian
systems, or osteons (B). The resident cells are
coated in a forest of cell membrane receptors
that respond to specific binding sites (C) and
the well-defined nanoarchitecture of the
surrounding extracellular matrix (D).

Science 18 November 2005:Vol. 310. no. 5751, pp. 1135 - 1138

Molly M. Stevens and Julian H. George
Exploring and Engineering the Cell Surface Interface

Natural and artificial fiber self-assembly.

(A) Natural assembly of collagen fibers:
(a) Pro-alpha chains are synthesized in
the lumen of the endoplasmic reticulum,
where (b) hydroxylation of selected
prolines and lysines occurs. (c) This is
followed by glycosylation of selected
hydroxylysines. (d) Three pro-alpha
chains self-assemble and (e) form a
procollagen triple helix. (f) These are
secreted into the extracellular space, and
(g) the protective propeptides are cleaved.
(h) Finally, the procollagen selfassembles into fibrils, and (i) these
aggregate into collagen fibers. (B)
Amyloid-like assembly: (a) Beta-sheet
membranes with hydrophilic and
hydrophobic faces group by (b) burying
their hydrophobic faces. In the correct
ionic solution, (c) the hydrophilic faces
attract and (d) the fibers self-assemble (9).
(C) Amphiphilic peptide assembly: (a)
Amphiphilic peptide design. (b) The
peptides bury their hydrocarbon tails, (c)
forming fibers in solution

Science 18 November 2005:Vol. 310. no. 5751, pp. 1135 - 1138

Molly M. Stevens and Julian H. George. Exploring and Engineering the Cell Surface Interface

Scaffold architecture affects cell binding and spreading. (A and B) Cells binding to scaffolds with
microscale architectures flatten and spread as if cultured on flat surfaces. (C) Scaffolds with nanoscale
architectures have larger surface areas to adsorb proteins, presenting many more binding sites to cell
membrane receptors. The adsorbed proteins may also change conformation, exposing additional cryptic
binding sites.

2009-10-19

Khmer woman who died of

hepatoma, four months after
arriving in a refugee camp in
Thailand CDC

2008

5
30

(Lancet)doi:10.1016/S0140-6736(08)61598-6Paolo MacchiariniMartin A
Birchall

Lancet
2008; 372: 202330

TransCyte
FDA
TransCyte

TransCyte
2 3


DMEM10%
DMEM+Ham F-12
PGA
-PGA
PGA

5
-
1

Body-Part Shack

Creative Biomolecules, Orquest,

Sulzer Orthopedics, Biologics,
Genetics Institute, Osiris
Therapeutics, Regeneron


Organogenesis
Apligraf
FDA

Organogenesis, Advanced Tissue

Sciences, Integra LifeSciences, LifeCell, Ortec
International

BioHybrid Technologies, Neocrin,

Circe Biomedical


10

Organogenesis, Advanced Tissue

Sciences, Genetech, LifeCell, Reprogenesis

Reprogensis, Integra LifeSciences

Genzyme Tissue, Biomatrix,

Integra LifeSciences, Advanced Tissue
Sciences, ReGen Biologics, Osiris
Therapeutics

Biora, Atrix Laboratories,

Creative BioMolecules

Reprogenesis, Integra LifeSciences



10

10
Advanced Tissue Sciences, Human
Organ Sciences, Organogenesis

Reprogenesis