Professional Documents
Culture Documents
Group 3
Draheim, Kristella M.
Estrella, Brian Nicole
Garcia, Kristine Micah Luisa
Guillermo, Linette
4BIO3
Introduction
The Winogradsky column is a
self-contained ecosystem wherein a
gradient is formed as a result of the
complex interaction of microbes
with their environment.
Introduction
Aerobic (oxygen rich) zone
1. photosynthetic cyanobacteria and algae
2. sulfur-oxidizing bacteria - Beggiatoa and
Thiobacillus
3. Purple non-sulfur bacteria
Anaerobic zone
1. sulfate-reducing bacteria such as
Desulfovibrio
2. anoxic phototrophs- purple sulfur
bacteria and green sulfur bacteria
3. fermentative heterotrophs such as
Clostridium
Bottom - H2S interacts with metals forming metal
sulfides
Results
Table 1. Observation with cover on
Observations
Odors
Color of the soil
Condensation on
plastic cover
Crust forming in
the bottle
Film on the
surface of the
water
Week 0
none
Grayishbrown
None
Week 1
foul
black
minimal
None
None
Clumps of
eggs
none
Week 2
Week 3
Week 4
Week 5
Strong pond
water smell Pungent odor
Green, pink
and gray
gray to black Gray to black Gray to black
pond water
smell
Pond water
smell
Water evident
on the cover
( plastic )
( very moist )
Many
minimal
many
Visible "green
matter " on
the surface Lesser green Green matter White and
on the edge green clumps
clumps
( might be
algae )
White black
White clumps
White and
and green
with green
Surface has
black clumps
clumps
matter
clumps
Results
Table 2. Observation with cover off
Observations
Odors
Week 0
Week 1
Week 2
sewage odor
sewage odor
Light gray
Green crust
Light gray
None
None
None
Macroscopic organisms
None
molds
Results
Week 0
Ssmo - 150mL
Hclmo - 500mL
Results
Table 2. Observation with cover off
Observations
Odors
Week 0
Week 1
Week 2
sewage odor
sewage odor
Light gray
Green crust
Light gray
None
None
None
Macroscopic organisms
None
molds
Results
Week 1
c. Condensation on
plastic cover
d. Microorganisms
(aerobic)
Results
Table 2. Observation with cover off
Observations
Odors
Week 0
Week 1
Week 2
sewage odor
sewage odor
Light gray
Green crust
Light gray
None
None
None
None
molds
Results
Week 2
c. Condensation on
plastic cover
d. Microorganisms
(aerobic)
Results
Table 2. Observation with cover off
Observations
Odors
Week 3
Week 4
Week 5
Intense stench
Mixture of light gray
and red
Faint stench
Sewage odor
Red
none
Molds
Results
Week 3
c. Condensation on
plastic cover
d. Microorganisms
(aerobic)
Results
Table 2. Observation with cover off
Observations
Odors
Week 3
Week 4
Week 5
Intense stench
Mixture of light gray
and red
Faint stench
Sewage odor
red
none
Molds
Results
Week 4
c. Condensation on
plastic cover
d. Microorganisms
(aerobic)
Results
Table 2. Observation with cover off
Observations
Odors
Week 3
Week 4
Week 5
Intense stench
Mixture of light gray
and red
Faint stench
Sewage odor
red
none
Molds
Results
Week 5
c. Condensation on
plastic cover
d. Microorganisms
(aerobic)
Results
The colors green, white and red/brown were observed
corresponding to the proliferation of eukaryal algae, cyanobacteria,
or thiobacilli, and sulfur oxidizing bacteria in the aerobic zone.
Oxygenic photosynthesis and respiration is also evident in the
areobic zone.
As for the anaerobic zone, the color red was observed suggesting the
presence of purple sulfur bacteria, an anoxic phototroph. Black color
was also observed at the bottom of the column indicating metal
sulfides corresponding to the presence of H2S produced by sulfate
reducers.
Gas, in the form of bubbles, in the anaerobic zone can be CH 4 from
methanogensis.
Conclusion
In the experiment, the Winogradsky column
demonstrated metabolic activities of diverse
microorganisms in response to different
environmental conditions. The column was
exposed to sunlight without cover for 6 weeks,
giving way to proliferation of microorganisms in
distinct zones where the environmental
conditions favor their specific activities.