2.49 desorbe experiments to investigate the elect of fight on net gas exchange from alleat, using hydrogen-carbonete indicator Aleatiplant can be pleced in a sealed N {ube of ait-equilbrated nydrogen . < carbonate solution (ed in colour) and fl placed in the light or in the dark. ei Seems i « Sf! Method: Setup the apparatus below according to the diagram. The tubes should be left for ‘soveral hours, ideally atleast 24 hours. Expected results: ‘+ Tube A The hydrogen carbonate Indicator tums yellow due to insreseed level: of carben doxide. The leaf can only respite die to a lack of Igntenergy. This results in pkeatorsaon were ath a production of carbon dioxide * Tube B—No colour change. This tube is a control to check that allthe colour changes that occur ate due the presence of the leat + Tube C—The hydrogen carbonate indicaior turns purple due jo decreased levels of carbon dioxide. The eal is resoling al the same rate as ihe bat in tube A. but due to higher leves of photosyrthesis more carbon dioxide is being used as a reactant (in photosynthesis) than is being produced (by respiration), + Tube D2? — ideelly 2 second control shoul be setup identically to tube 8, but additionally wrapped in fll 20 that lke tube A it weeeives ro light. pent CL stumewn fo Variants and extensions: + Smallinveriebrates (on a gauze platform) can be used to show the effect of respiration relative to photosynthesis + Waterwoeds can bo ueod hstead of leaves ‘ Simiery fo the small inverteorates water snails can be used with the walenweed to stow the: effect of respiration relative to photosynthesis 1b. using animals they must not be harmed during the experiment and the conditions they ‘are subjecied 120 must ones normally experienced in thelr natural environments. Weaknesses: ‘= Hoalth and size of plants varios. Therofore tho rato of rospiration and photosynthes will also vary, Keally repeats should be used to make the Investigation more reliable + Theetfec’ of night-time respiration may be laigerthan the net reduction of carbon dioxide during the day meaning tube C as well as tube A contains yellow hydrogen cartonele Indicator solution. An artiicial lant should be used to make sure that this Coes not happen. + Due to use of the waterbath and placement in the room tube C may roseive minimal ight levels and hence photosynthesis may not be greater than respiration. As previously an artificial ight stould be used