rRNA

(ribosome) rRNA

()

70S

2.7 x 106

80S

4.6 x 106

30S

0.9 x 106

40S

1.5 x 106

rRNA

16S

0.6 x 106

18S

0.7 x 106

21

0.3 x 106

30

0.78 x 106

50S

2.0 x 106

60S

3.0 x 106

rRNA

23S

1.2 x 106

28S

1.7 x 106

5.8S

4.0 x 104

5S

3.2 x 104

5S

3.2 x 104

34

0.7 x 106

50

1.37 x 104

rRNA(pre-rRNA)
45S(1300 )
rRNA 28S rRNA 18S
rRNA 4,718 1,874 5.8S
rRNA 156 23S
rRNA5' 156
25S 17S rRNA
28S 18S rRNA5S rRNA
120 16S rRNA 23S
rRNA 1978 1,542
2,904
rRNA

48

(1) rRNA RNA

(2) G-C A-U
(3) rRNA

(4) rRNA 4 ()
()
(5) rRNA rRNA
() rRNA RNA 16S 3'
mRNA (SD )
rRNA rRNA
( 3 mol/L LiCl 4 mol/L )
""("real ribosomal proteins")
E.coli 30S 21 50S 34 ( 54
S20 L26 ) 40S
30 60S 45-50 ( 80 )
0.5 mol/L (
K+NH4+)
(IF eIF)(EF)(proteins associated with
ribosome PAR)PAR

E.coli
E.coli 54 rRNA
(architecture)
:
(1) (EM)
(2)
(3) (neuton scattering)
(4)
(5) (singlet-singlet energy transfer)
(6)
1.

49

 X
X
30S 5.5 22 22 nm50S
11.5 23 23 nm
50S (trilobal
hemi spherical model)RNA
RNA

EM EM EM
EM

1/3 2/3
1-2 EM

(quasi-symmetric"crown")
""
(stalk)(ridge) L7/L12
8-12 nm L1 L1 (L1shoulder) 70S
23 nm(45 ) 50S L1

(diffraction) X
1979-1982

2.

L7/L12 C 50S N L10 (L10

23S rRNA )
mRNAtRNA
()
RNA
2 3
(L7/L12)2(L7/L12)4
L10 (L7/L12)4-L10

50

 S13-S19S3-S4S3-S5S3-S5-S10S4-S5S4-S20S5-S8 S5-S10

3.

rRNA

rRNA
70S rRNA
16S rRNA V 23S 50S"
"rRNA
rRNA
rRNA N66-( 16SrRNA3' 24 25
)( 16S rRNA3' 25 ) 30S
16S rRNA 526 m7G 30S 1/3 2/3 16S5S
23S rRNA 5S rRNA G41 G72
5S rRNA
RNA rRNA

rRNA
rRNA rRNA
rRNA
(rDNA)()

(self-assembly)
1968
rRNA
16S rRNA 21 (S1-S21)
30S
rRNA

2/3 30S
16SrRNA
21S 37
21S
26S( 1/3)
26S 30S 4-41a
30S 16SrRNA (assmblymap)

51

 : 7 S 16SrRNA rRNA
S3
S6S10S14S18 S21

E.coli
444mmol/L Mg2+
33S41S 48S33S
rRNA(23S5S) 2/3 50S 44
33S 41S41S 48S
5048S 50S
20 23S rRNA
(L24 L3) 33S 20 5
41S L20 L24
41S 41S 50S
50S
23S rRNA 5'
3' RNA

50S L20 L15

L20
L15
DNA

(S)(L)
mRNAtRNA

1 mRNA 16S rRNA 3' 30S

2 tRNA 30S 1/3 (cleft)

3 GTPase L7/L12[(L7/L12)4] 50S

4 ()
5 30

52


6 50S
7 mRNA tRNA 30S
GTPase 50S

X-

53