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INTRODUCTION
Carbohydrates, also known as saccharides, are
carbon compounds that contain large quantities of
hydroxyl groups, and are also the most important
sources of energy.
They have the basic general
formula Cn(H2O)n and they are the most commonly
found organic compounds in living organisms. They
are
classified
into
several
groups,
namely
monosaccharides, disaccharides, and polysaccharides,
depending on the number of their monosaccharide
units.
EXPERIMENTAL
A. Materials
1% starch
B. Procedure
For Isolation of Glycogen
Weigh 3 g of chicken liver using an analytical
balance and place on a Petri dish. Cut the sample with
the use of scissors. Transfer sample to a beaker and
pour 12 mL of distilled boiling water. Stir contents with
a glass rod and boil for two minutes using a hot plate
to precipitate the proteins in the sample. Pour the
mixture into a mortar and use pestle to grind the
sample thoroughly until no lumps are visible. Add 3
mL distilled water and transfer the mixture into a
beaker. Heat the mixture in a boiling water bath for
thirty minutes and, if necessary, add distilled water to
the mixture to avoid evaporation since glycogen goes
to the solution when heating. Filter solution using filter
paper and separate glycogen samples into four
portions and transfer to test tubes.
*0.1% acetic acid may be added to improve
precipitation of proteins during heating of sample in
boiling water bath.
Carbohydrat
e Solution
Benedicts
Barfoeds
Seliwanoffs
Bials
Glucose
Fructose
Xylose
Lactose
Sucrose
Starch
Glycogen
Hydrolysate
(+)
(+)
(+)
(+)
()
()
()
(+)
(+)
(+)
()
()
()
()
()
(+)
(+)
(+)
(+)
(+)
()
(+)
()
(+)
()
()
()
(+)
Results
Molischs Test
I2 Reaction
Visible Results
Figure
Tests
5.
Positive
Results
for
Bials
Figure
4.
Positive
Seliwanoffs Test
Results
for
in
REFERENCES
[1]
Crisostomo, A., et. al. (2010).
Labaratory Manaul in Genearal Biochemistry.
Quezon City: C&E Publishing Inc., pp. 87-92
[2] http://himedialabs.com/TD/HTBC002.pdf
[3]http://www.biosci.ohiou.edu/introbioslab/Bi
os170/170_2/benedict.htm
[4]http://www.slideshare.net/katealyssacaton
/mucic-and-barfoeds-test
[5]http://www.harpercollege.edu/tmps/chm/1
00/dgodambe/thedisk/carbo/bial/bials.htm