Professional Documents
Culture Documents
Name: ______________________________________
Date: ___________
Objectives:
Tounderstandhowwatermovesfromrootstoleavesintermsofthe
physical/chemicalpropertiesofwaterandtheforcesprovidedbydifferencesinwater
potential.
Tounderstandtheroleoftranspirationinthetransportofwaterwithinaplant.
Tounderstandthestructuresusedbyplantstotransportwaterandregulatewater
movement.
Totesttheeffectsofenvironmentalvariablesonratesoftranspirationusinga
controlledexperiment.
Toidentifyxylemandphloemcells,andrelatethefunctionofthesevasculartissues
tothestructuresoftheircells.
Introduction:
SeeCollegeBoardLabManual
Procedure:
1. Wraptheentirerootballinaplasticbag,snugituptothestemwithstring,markwith
yourgroupnameornumber,andweighonMonday.Weigheachsuccessivedayforthe
entireweek.
2. Allgroupsputtheircontrolsinoneplace,thoseinfrontofthefantogether,thosein
brightlighttogether,andthelastaremistedandcoveredwithanotherplasticbagand
areplacedwiththecontrols.Darkareplacedinadrawerintheroom.
3. Ifyourplantblooms,pinchthebloomsoffandbesureanyleavesorbloomsthatfalloff
areputbackinthecenteroftheplanttobeweighedeachdaysoasnottorepresentwater
loss.
4. Writeahypothesisaboutwhatyouthinkwhatwillhappenbasedonyourknowledgeof
transpirationandplants.(1)
__________________________________________________________________________
__________________________________________________________________________
Results:
Fillinthedatatable.Besuretousethecorrectunitsformass.
DAY0
DAY1
DAY2
(5)
DAY3
DAY4
CONTROL
HUMIDITY
LIGHT
FAN
DARK
Determinethe%changeinmassovertheweekandgraph.Besureyourgraphhasallofthe
appropriatetitlesandunits. (8)
TITLE:________________________________________________________________
Analysis:(FromtheCollegeBoardLabManual)
(36pointstotal)
1. Forthisexperiment,whatwastheindependentvariable?Thedependentvariable?What
weretheconstants? (4)
2. Calculatetheaveragerateofwaterlossperminuteforeachofthetreatments.(4)
a. Humidity
b. Light
c. Fan
d. Dark
3. Explainwhyeachoftheconditionscausesanincreaseordecreaseintranspiration
comparedwiththecontrol. (4)
a. Humidity
b. Light
c. Fan
d. Dark
4. Howdideachconditionaffectthegradientofwaterpotentialfromthestemtoleafinthe
experimentalplant? (4)
a. Humidity
b. Light
c. Fan
d. Dark
5. Whatistheadvantagetoaplantofclosedstomatawhenwaterisinshortsupply?What
arethedisadvantages?
(3)
6. Describeseveraladaptationsthatenableplantstoreducewaterlossfromtheirleaves.
Includebothstructuralandphysiologicaladaptations.
(4)
7. Whydidyouneedtocalculatethe%waterlosseachdayinsteadofgraphingthetotal
amountofwaterlosteachday?
(1)
8. CalculatethetotalsurfaceareaofalltheleavesbyusingtheLeafMassMethoddescribed
intheCollegeBoardlab.
(1)
LeafSurfaceArea(m2)=__________________
9. Waterlosspersquaremeter:Tocalculatethewaterlosspersquaremeterofleafsurface,
dividethewaterloss(changeinmassingrams)ateachreadingbytheleafsurfaceyou
calculated.(Rememberthat1gram=1milliliter)
IndividualGroupWaterLossinmL/m2
DAY1
(5)
DAY2
DAY3
CONTROL
HUMIDITY
LIGHT
FAN
DARK
10. Recordtheaveragesoftheclassdataforeachtreatmentinthetablebelow.
ClassAverageCumulativeWaterLossinmL/m2(5)
DAY4
DAY1
DAY2
DAY3
CONTROL
HUMIDITY
LIGHT
FAN
DARK
11.Whydidyouneedtocalculateleafsurfaceareaintabulatingyourresults? (1)
DAY4