£ @ B.Tech / Even « B.Tech /Even_ (2010-11) / Reg. 2010-11, PLANT BIOTECHNOLOGY BT -602 Full Marks : 70 Time : 3 hours Figures in the margin indicate marks for respective questions. Answer any seven questions. 1 (@) Whatis plant tissue culture? (b) What are the general requirements to establish a plant tissue culture laboratory ? (©) Differentiate between major and minor elements high- lighting examples ? (@) While preparing tissue culture media gelrite is a good alternative to agar as a gelling agent. Explain why ? 2444242210 2. (a) Define cellular totipotency and somatic embryogenesis. (6) Compare somatic embryos vs. zygotic embryos. (c) Describe the factors affecting yield and viability of protoplasts. 34+9+4=10 8. (a) Design a genetic screen to identify a mutant that cannot see blue light. (®) How do you clean up the background of the isclated ‘mutant line ? B+5=10 4. (a) What is complementation group? an2-60 [Turn Over |(b) How do you determine the complementation group of 6 mutants obtained from a genetic screen ? 3+7=10 5. (a) What is a regulatory protein? (®) Briefly describe a method to clone a gene that encodes a regulatory protein by reverse genetics approach. 3 +7= 10 6. How do you clone a full-length DNA? 10 7. (@) Define molecular markers and conventional markers. (©) What are the differences between DNA markers and isozymes/allozymes ? (c) What are the advantages of using DNA markers over conventional markers ? (@) Explain why it is required to map genes ? 2424442210 8. Explain the following : (@) Gene @®) Mt (©) High density map (@) Gene tagging () SNP 2x5=10 9. Distinguish between the following : (a) Genetic map and Physical map (6) Major genes and Minor genes (©) RFLP and AFLP (d) SSCP and SSLP. 25x4=10 10. (a) What are the advantages of genetic transformation of plants over conventional plant breeding programmes ? G02-60 [Continued | o® (6) List the names of different direct and indirect methods: of genetic transformation of plants. (c) Briefly discuss any three nonstandard seat that nay be used for genetic engineering ofplants.. | What i ‘using Agrobacterium 4 are the major advantages of u U1. fo) ociens in genetic transformation of plants over microprojectile delivery ? - () Discuss how the virulence system of this bacterium 1S induced ? (©). What is disarming of Ti-plasmid ? (@ What is triparental mating? B444242=10